Green synthesis and characterization of silver nanoparticles using Morinda lucida leaf extract and evaluation of its antioxidant and antimicrobial activity

Abstract

This study emphasizes the production of eco-friendly silver nanoparticles from a medicinal plant extract of Morinda lucida (M. lucida) and investigated its antioxidant and antimicrobial activity. Phytochemical screening of M. lucida (ML) leave extract was carried out and observed to contain some fundamental phyto-reducing agents such as reducing sugar, proteins, and alkaloids. The green synthesized AgNPs (ML-AgNPs) were characterized by UV–vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), transmission emission microscopy (TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), and Energy dispersive X-ray analysis (EDX). Thermo gravimetric analysis (TGA) was performed on the synthesized ML-capped AgNPs to determine the thermal stability and the formation of the green synthesized AgNPs. The formation of AgNPs was confirmed by the UV–vis absorption spectra, which showed an absorption band at 420 nm. The morphology of ML extract-mediated AgNPs was mostly spherical and rough-edged crystallite nanostructures, with an average particle size of 11 nm. The FTIR analyses revealed distinctive functional groups which were directly involved in the synthesis and stability of AgNPs. The crystallite size was 8.79 nm, with four intense peaks at 2 θ angles of 38°, 44°, 64°, and 77°. At an energy level of 3.4 keV, a significant signal was observed indicating the production of thermally stable and pure crystallite AgNPs. The antioxidant property of green synthesized ML-AgNPs was determined to be 40% higher than that of crude M. lucida leaf extract. The ability of green synthesized ML-AgNPs to scavenge free radicals also increased in the order of OH − < NO < H 2 O 2 . The ML-AgNPs have strong activities with a maximum against P. vulgaris and a minimum with E. faecalis.

Synthesis of biogenic silver nanoparticles from the extract ofHeliotropium eichwaldiL. and their effect as antioxidant, antidiabetic, and anti‐cholinesterase

“…[12] The current results are also supported previously. [85] Nutritional antioxidants have been reported to have a significant effect against the pancreatic α-amylase enzyme, an enzyme that breaks down the large polysaccharides into small pieces. It seems believable that an appropriate antioxidants consumption could play a significant role in the management of type 2 diabetes.…”

Anatomical adaptation of water‐stressed Eugenia uniflora using green synthesized silver nanoparticles and melatonin

“…The AgNPs were produced using M. lucida (ML) leaf extract, as described in our recent work (Labulo et al, 2022). In a typical experiment, 10 mL of aqueous M. lucida leaf extract and 40 mL of 1 mM AgNO 3 are combined at room temperature.…”

Identification and Pharmacological Activities of Secondary Metabolites of Phytochemicals

“…When it produces an emulsion is hydrolyzable tannins. foam [79,105], or honeycomb froth [108,[111][112] showed the presence of saponins glycosides. This method determines the total phenolic compound in plant crude extract where the Folin-Ciocalteu reagent (2.5 g Na2MoO4.2H2O, 10 g Na2WO4.2H2O in 70 mL distilled H2O, then 5 mL 85% H3PO4 and 10 mL HCl), a mixture of phosphomolybdate and phosphotungstate reagents (H3PMo12O40 and H3PW12O40) and aluminum chloride (AlCl3), in the presence of phenolics and carried out in alkaline medium Na2CO3 to produce a blue color molybdenum tungsten (Mo8O23 and W8O23) that can be measured in the range of 720-765 nm in visible spectrophotometer against the standard gallic acid.…”

“…Lead acetate test: Diluted plant extract and lead acetate to give black-green colored precipitate formed revealed the presence of tannins[104]. The yellow precipitate indicates flavonoid[105,111,112]. 4.2.2.4.…”