Green synthesis and cytotoxicity evaluation of silver and gold nanoparticles using Pandanus canaranus leaf extract on Artemia salina and A549 lung cancer cells

Balamurugan, Venkatachalam; Balasubramani, Govindasamy

doi:10.1007/s13399-022-03586-8

Cited by 5 publications

(2 citation statements)

References 65 publications

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“…Artemia has several biological advantages and unique life phenomena, such as a transparent body, asexual and bisexual reproduction modes, oviparity and ovoviviparity breeding strategies, and diapause and de-diapause life cycles [14]. Therefore, Artemia has been used as an experimental animal in environmental toxicology, oncology, epigenetics and other research fields [15][16][17]. Compared to bisexual Artemia, parthenogenetic Artemia (A. parthenogenetica) imposes a special reproduction mode.…”

Section: Introductionmentioning

confidence: 99%

Spatio-temporal patterns of ovarian development and VgR gene silencing reduced fecundity in parthenogenetic Artemia

Duan,

Shao,

Liu

et al. 2023

Open Biol.

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The halophilic zooplankton brine shrimp Artemia has been used as an experimental animal in multidisciplinary studies. However, the reproductive patterns and its regulatory mechanisms in Artemia remain unclear. In this study, the ovarian development process of parthenogenetic Artemia ( A. parthenogenetica ) was divided into five stages, and oogenesis or egg formation was identified in six phases. The oogenesis mode was assumed to be polytrophic. We also traced the dynamic translocation of candidate germline stem cells (cGSCs) using EdU labelling and elucidated several key cytological events in oogenesis through haematoxylin and eosin staining and fluorescence imaging. Distinguished from the ovary structure of insects and crustaceans, Artemia germarium originated from ovariole buds and are located at the base of the ovarioles. RNA-seq based on five stages of ovarian development identified 2657 upregulated genes related to reproduction by pair-to-pair comparison. Gbb , Dpp , piwi , vasa , nanos , VgA and VgR genes associated with cGSCs recognition and reproductive development were screened and verified using qPCR. Silencing of the VgR gene in A. parthenogenetica ( Ap-VgR ) at ovarian development Stage II led to a low level of gene expression (less than 10%) within 5 days, which resulted in variations in oogenesis-related gene expression and significantly inhibited vitellogenesis, impeded oocyte maturation, and eventually decreased the number of offspring. In conclusion, we have illustrated the patterns of ovarian development, outlined the key spatio-temporal features of oogenesis and identified the negative impacts of VgR gene knockdown on oogenesis using A. parthenogenetica as an experimental animal. The findings of this study also lay a foundation for the further study of reproductive biology of invertebrates.

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Section: Introductionmentioning

confidence: 99%

Spatio-temporal patterns of ovarian development and VgR gene silencing reduced fecundity in parthenogenetic Artemia

Duan,

Shao,

Liu

et al. 2023

Open Biol.

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“…Artemia has several biological advantages and special life phenomena, such as transparent body, asexual and bisexual reproduction modes, oviparity and ovoviviparity breeding strategy as well diapause and de-diapause life cycles (MacRae, 2016). And thus, Artemia have been used as an experimental animal in environmental toxicology, oncology, epigenetics and other research fields (Ye et al, 2017;Suman et al, 2020;Balamurugan and Balasubramani, 2022). Compared with bisexual Artemia, parthenogenetic Artemia (P.…”

Section: Introductionmentioning

confidence: 99%

Spatio-temporal patterns of ovarian development andVgRgene silencing reduced fecundity of parthenogeneticArtemia

Duan

Shao²,

Liu³

et al. 2023

Preprint

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Halophilic zooplankton brine shrimp Artemia has been used as an experimental animal in multidisciplinary research. However, the reproductive patterns and regulation mechanisms of Artemia remain a mystery. In this study, the ovarian development process of parthenogenetic Artemia (P. Artemia) was distinguished into five stages, and oogenesis or egg formation process was identified with six phases. The mode of oogenesis was assumed polytrophic. Moreover, we traced the dynamic translocation of germline stem cells (GSCs), elucidated several key cytological events in oogenesis. Notably, distinguished from the ovary structure of insects and crustaceans, P. Artemia germarium originated from ovariole buds and located in the base of the ovarioles. RNA-seq revealed that 2657 up-regulated genes related to reproduction were obtained. Furthermore, Gbb, Dpp, piwi, vasa, nanos, VgA and VgR were screened and also verified by qPCR. Silencing of the VgR gene in P. Artemia (PA-VgR) at stage Ⅱ of ovarian development led to a low level of gene expression (＜10%) within five days, which resulted variations of oogenesis-related gene expression and significantly inhibited vitellogenesis, impeded oocyte maturation, and eventually decreased the offspring number. In conclusion, we illustrated the patterns of ovarian development, outlined the key spatio-temporal features of oogenesis and established a platform for gene function research using P. Artemia as an experimental animal. These results lay the foundation for studying the reproductive biology of invertebrates, may also provide a novel insight in the reproductive regulation mechanism of aquatic animals in extreme environments.

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Synthesis of Gold Nanoparticles Using Leaf Extracts of Pterospermum Canescens and Vernonia Elaeagnifolia: Characterization, Platelet Aggregation and Cytotoxic Studies

Sherin,

Jayakumar,

Anitha

et al. 2024

ChemistrySelect

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Gold nanoparticles are extensively used across various industries, particularly in biomedical fields, due to their high biocompatibility, stability, and low toxicity. The rising use of gold nanoparticles in biomedical applications has increased the demand for more sustainable synthesis methods that reduce costs and environmental impacts compared to traditional chemical processes. This study presents a green synthesis approach utilizing leaf extracts from Pterospermum canescens and Vernonia elaeagnifolia. The synthesized nanoparticles were characterized using UV‐VIS, XRD, HRTEM‐SAED, FESEM‐EDX, XPS, and FTIR analyses. These characterizations confirmed the formation, size, shape, and morphological features of the nanoparticles, as well as the involvement of secondary plant metabolites in their synthesis. The nanoparticles were found to be less than 50 nm in size, with P.C AuNPs averaging 27.48 nm and V.E AuNPs averaging 14.79 nm. The particles predominantly exhibited spherical or distorted spherical shapes. In cytotoxicity assays using C6 rat glioma cells, both types of nanoparticles demonstrated significant effects. V.E AuNPs induced apoptosis in 78 % of the cells, while P.C AuNPs induced apoptosis in 50 %, confirming their cytotoxic efficacy. Gene expression studies further revealed that V.E AuNPs significantly upregulated caspase‐3 and downregulated BCL‐2, indicating a potent apoptotic response, while P.C AuNPs showed a similar but less pronounced effect. Additionally, in collagen‐induced platelet aggregation studies, V.E AuNPs exhibited substantial anti‐platelet activity with increasing dosages, reducing platelet aggregation to less than 10 % at a concentration of 30 μg/ml, compared to minimal inhibition by P.C AuNPs. Overall, the study demonstrates that gold nanoparticles synthesized from Vernonia elaeagnifolia and Pterospermum canescens are effective and offer promising applications in biomedicine. The results confirm the potential of these green‐synthesized nanoparticles for use in cancer treatment and sustainable anti‐platelet drug development

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Green synthesis and cytotoxicity evaluation of silver and gold nanoparticles using Pandanus canaranus leaf extract on Artemia salina and A549 lung cancer cells

Cited by 5 publications

References 65 publications

Spatio-temporal patterns of ovarian development and VgR gene silencing reduced fecundity in parthenogenetic Artemia

Spatio-temporal patterns of ovarian development and VgR gene silencing reduced fecundity in parthenogenetic Artemia

Spatio-temporal patterns of ovarian development andVgRgene silencing reduced fecundity of parthenogeneticArtemia

Synthesis of Gold Nanoparticles Using Leaf Extracts of Pterospermum Canescens and Vernonia Elaeagnifolia: Characterization, Platelet Aggregation and Cytotoxic Studies

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