Green Synthesis and Evaluation of &lt;i&gt;Lepidium didymum&lt;/i&gt;-mediated Silver Nanoparticles for &lt;i&gt;in vitro&lt;/i&gt; Antibacterial Activity and Wound Healing in the Animal Model

Deeba, Farah; Parveen, Sobia; Rashid, Zermina; Aleem, Ambreen; Raza, Hina

doi:10.5650/jos.ess22380

Cited by 5 publications

(2 citation statements)

References 41 publications

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“…4–0783. 44 Similar XRD patterns have been observed in other studies, 45 confirming the successful preparation of PCPs using green synthesis methods. 46 The TEM technique was used to determine the size, shape, and morphology of silver nanoparticles synthesized in situ, 47 and the resulting plot ( Figure 2D ) showed that the synthesized PCPs were predominantly spherical and well dispersed.…”

Section: Resultssupporting

confidence: 83%

Pycnoporus sanguineus Polysaccharides as Reducing Agents: Self-Assembled Composite Nanoparticles for Integrative Diabetic Wound Therapy

Huang,

Shi,

Lin

et al. 2023

IJN

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Purpose Diabetic foot ulcers (DFU) are severe complications of diabetes, posing significant health and societal challenges. Elevated levels of reactive oxygen species (ROS) at the ulcer site hinder wound healing in most patients, while individuals with diabetes are also more susceptible to bacterial infections. This study aims to synthesize a comprehensive therapeutic material using polysaccharides from Pycnoporus sanguineus to promote DFU wound healing, reduce ROS levels, and minimize bacterial infections. Methods Polysaccharides from P.sanguineus were employed as reducing and stabilizing agents to fabricate polysaccharide-based composite particles (PCPs) utilizing silver ions as templates. PCPs were characterized via UV-Vis, TEM, FTIR, XRD, and DLS. The antioxidant, antimicrobial, and cytotoxic properties of PCPs were assessed through in vitro and cellular experiments. The effects and mechanisms of PCPs on wound healing were evaluated using a diabetic ulcer mouse model. Results PCPs exhibited spherical particles with an average size of 57.29±22.41 nm and effectively combined polysaccharides’ antioxidant capacity with silver nanoparticles’ antimicrobial function, showcasing synergistic therapeutic effects. In vitro and cellular experiments demonstrated that PCPs reduced cellular ROS levels by 54% at a concentration of 31.25 μg/mL and displayed potent antibacterial activity at 8 μg/mL. In vivo experiments revealed that PCPs enhanced the activities of superoxide dismutase (SOD) and catalase (CAT), promoting wound healing in DFUs and lowering the risk of bacterial infections. Conclusion The synthesized PCPs offer a novel strategy for the comprehensive treatment of DFU. By integrating antioxidant and antimicrobial functions, PCPs effectively promote wound healing and alleviate patient suffering. The present study demonstrates a new strategy for the integrated treatment of diabetic wounds and expands the way for developing and applying the polysaccharide properties of P. sanguineus .

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Section: Resultssupporting

confidence: 83%

Pycnoporus sanguineus Polysaccharides as Reducing Agents: Self-Assembled Composite Nanoparticles for Integrative Diabetic Wound Therapy

Huang,

Shi,

Lin

et al. 2023

IJN

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“…This green technology approach is environmentally friendly and produces G-SNs of a consistent size [16]. The G-SN approach can inhibit bacterial and fungal growth, including E. coli and Bacillus subtilis [17,18]. The toxicity of chemically synthesized NPs is considered a substantial challenge that would be prevented by the production and use of G-SN [19].…”

Section: Introductionmentioning

confidence: 99%

Eco-Friendly Synthesis of Silver Nanoparticles by Nitrosalsola vermiculata to Promote Skin Wound Healing

et al. 2023

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Eco-friendly synthesis of silver nanoparticles (SN) by using a naturally occurring plant, such as Nitrosalsola (Salsola) vermiculata (SV), could be a novel way for appropriate wound healing. AgNO3 was reduced by SV to produce safe SN (SN-SV) extract and hasten the wound healing process. The obtained SN-SV were characterized by size, charge, wavelength, and surface morphology. The optimized formulation was dispersed in O/W cosmetic cream. Then, it was characterized in terms of pH, viscosity, homogeneity, and permeability. The ex vivo and in vivo studies have been conducted in a rat animal model to assess the potential of SN-SV cream on skin tissue regeneration. A skin punch biopsy was obtained to investigate the histopathological (HP) changes in the skin lesions of all rats by the H&E staining and PCNA immunostaining methods. The skin wounds in all subgroups were examined on days 5, 11, and 15 to analyze the effectiveness of SN-SV cream for treating surgical skin wounds. The prepared SN-SV had a particle size of 37.32 ± 1.686 nm, a charge of −1.4 ± 0.7 mV, non-aggregated SN-SV, and a λmax of 396.46 nm. The formed SN-SV cream showed a pH near the skin’s pH, with suitable viscosity and homogeneity and an apparent permeability of 0.009 ± 0.001. The HP changes in the SN-SV subgroups revealed a substantial reduction in wound size and improvement in wound granulation tissue formation and epidermal re-epithelialization (proliferation) compared to the healing in the SN subgroups. The current work revealed that SN-SV could be a novel skin-wound-healing agent with a practical application as a wound-healing platform.

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The genus Lepidium L. (Brassicaceae): A comprehensive review of botany, traditional uses, pharmacology, phytochemistry and the possible toxicities

Jouybari,

Amini,

Mirzaee

2024

South African Journal of Botany

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Green Synthesis and Evaluation of <i>Lepidium didymum</i>-mediated Silver Nanoparticles for <i>in vitro</i> Antibacterial Activity and Wound Healing in the Animal Model

Cited by 5 publications

References 41 publications

Pycnoporus sanguineus Polysaccharides as Reducing Agents: Self-Assembled Composite Nanoparticles for Integrative Diabetic Wound Therapy

Pycnoporus sanguineus Polysaccharides as Reducing Agents: Self-Assembled Composite Nanoparticles for Integrative Diabetic Wound Therapy

Eco-Friendly Synthesis of Silver Nanoparticles by Nitrosalsola vermiculata to Promote Skin Wound Healing

The genus Lepidium L. (Brassicaceae): A comprehensive review of botany, traditional uses, pharmacology, phytochemistry and the possible toxicities

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