The use of fungi in the safe, economical, and ecologically benign synthesis of silver nanoparticles is emerging as a major field in nanotechnology. The fungus Fusarium oxysporum is used in the current study to investigate the biological synthesis of silver nanoparticles. Since putrefying banana fruit, the assessment fungus that was derivative after PDA was inaccessible. Proceeding the foundation of morphologic traits, Fusarium oxysporum was acknowledged. The mechanism of silver nanoparticle making by the fungus Fusarium oxysporum was considered. The situation remained originate that as soon as exposed to silver ions, Fusarium oxysporum harvests silver nanoparticles. When the produced nanoparticles were examined using UV-Vis spectroscopy, the peak of the spectra was found to be at 420 nm. Silver nanoparticles were subjected to a TEM-based morphological analysis, which revealed that the particles are spherical in shape and have a diameter of between 50 and 100 nm. The TEM analysis of the fungus's response to the silver ion suggests that the protein may be in charge of stabilizing the silver nanoparticles. A large-scale biosynthesis process for "microbial nanotechnology" would benefit greatly from the speedy synthesis of silver nanoparticles.