Green Synthesis, Characterization, Antimicrobial, Anti-Cancer, and Optimization of Colorimetric Sensing of Hydrogen Peroxide of Algae Extract Capped Silver Nanoparticles

Abstract

A green and cost-effective technique for the preparation of silver nanoparticles (Algae-AgNPs) as a colorimetric sensor for hydrogen peroxide (H2O2) is described. Silver nanoparticles were capped using the green algae (Noctiluca scintillans) extract at an optimum time of 3 h at 80 °C. The pH of the plant extract (pH = 7.0) yields nanoparticles with a mean size of 4.13 nm and a zeta potential of 0.200 ± 0.02 mV and negative polarity, using dynamic light scattering (DLS). High-resolution transmission electron microscopy (HRTEM) analysis showed regular spherical particles with the average size of 4.5 nm. Selected area electron diffraction (SAED) results revealed the polycrystalline nature of the silver nanoparticles. The obtained patterns were indexed as (111), (200), (220), and (311) reflections of the fcc (face centered cubic) silver crystal based on their d-spacing of 2.47, 2.13, 1.49, and 1.27 Å, respectively. The apparent color change from brown to colorless was observed when nanoparticles reacted with H2O2. Linear responses were obtained in three different ranges (nM, µM, and mM). Limits of detection (LOD) of 1.33 ± 0.02 and 1.77 ± 0.02 nM and quantitation limits (LOQ) of 7.31 ± 0.03 and 9.67 ± 0.03 nM were obtained for Abs and ΔAbs calibration curves, respectively. 10% v/v Algae-AgNPs solution inhibited Staphylococcus aureus over Escherichia coli, while a 50% reduction of tumor cell growth of MDA-MB-231 human breast adenocarcinoma was obtained.

Optical Management of CQD/AgNP@SiNW Arrays with Highly Efficient Capability of Dye Degradation

“…In hybrid features of CQD/AgNP@SiNWs, several additional diffraction peaks at 38. [26,27]. In addition, the grain size of sole CQDs and CQD/AgNP structures are estimated using Scherrer's formula.…”

Marine Biological Macromolecules as Matrix Material for Biosensor fabrication

“…Marine Nanomaterial based biosensors should be integrated within tiny biochips, which enhances functionality of biosensors as well as made portable, pocket friendly and easy to use (Singh et al, 2015). Elgamouz et al (2020) have prepared Noctiluca scintillans -mediated AgNP's biosensor for sensing hydrogen peroxide. It was discovered that the breakdown of hydrogen peroxide on AgNP's catalytic surface is pH, temperature, and time-dependent.…”

Synthesis of Graphene Oxide from Hydrogenated Diamond Like Carbon and Protein Immobilization onto It: Characterization and Study of Practical Utility

“…Since the discovery of the many advantageous properties of immobilized biomolecules, the challenges in this area have been to develop new support substrate materials with appropriate structures, surface morphology and functionality to widen the understanding of immobilized biomolecules. Recently, along with the development of nanostructured materials [7], a range of nanomaterials with different surface properties have been utilized as substrates for biomolecule immobilization [8]. In particular, oxidized graphene [9] has been demonstrated as a well-defined, ordered, and atomically smooth surface [9].…”