Green Synthesis of Biodiesel Using Microbial Lipases

Magnetic Graphene Oxide as a Carrier for Lipases Immobilization: An Approach for Hydrolysis of Olive Oil Emulsion

“…However, studies involving the use of MGO with lipases are limited and generally only associated with porcine pancreatic lipase, 31,32 thus restricting the possibilities of applications to other sources of lipases, which are also widely applied in chemical, food and pharmaceutical sectors. 4 Thereby, this work describes the novelty of the use of graphene oxide decorated with MNP as a carrier for the immobilization of differents types of lipase to confirm its easy and advantageous interaction for the hydrolysis of olive oil emulsion. The catalytic performance of three immobilized lipases (Porcine pancreatic, Candida rugosa lipase and Aspergillus niger) were studied and compared to the free ones to access ideal pH and temperature conditions for transesterification reactions.…”

“…1,2 However, some enzymes have low yield extraction and difficult purifications procedures, which compromises their stability and catalytic activity. 3,4 In this way, to improve these properties, different materials have been studied as a carrier for specific enzymatic immobilization. 5 To enable an efficient carrier/enzyme interaction, the choice of a suitable immobilization process would improve the enzymatic activity, the thermal and storage stability, high amount of carried enzyme and its reuse.…”