Green synthesis of gold nanoparticles for staining human cervical cancer cells and DNA binding assay

Studying non-covalent drug–DNA interactions

“…NR binds to DNA in intercalative mode and results in enhancement of fluorescence intensity of NR on binding with DNA [43]. Small molecules can displace NR from DNA helix and decrease the emission intensity of DNA-NR systems only if they intercalate into the bases of DNA [43]. Acridine Orange (AO), a well known classical intercalating dye, is also used in similar competitive displacement assays [44,45].…”

Preliminary investigation on biological possessions of Saquinavir‐modified quinoline‐derived azadipeptidomimetics

“…The molar concentration of DNA was determined based on absorbance ratio A 260/280 and A 260/230 at 260 nm, the ratio maintained between 1.8 and 1.9. [ 33 ] The solution containing 5 × 10 −5 M of compound was titrated against DNA solution using spectrophotometer. The spectral absorption data from the titration were used to calculate the binding constant and dissociation constant.…”

Green Synthesis of Therapeutic Nanoparticles: an Expanding Horizon

“…Cadmium sulfide (CdS) nanotriangles were also synthesized via green route which exhibited strong quantum confinement effect as compared with the bulk CdS [67]. Biosynthesized AuNPs were found promising for staining human cervical cancer cells and also for DNA binding assay [52]. The authors observed staining property even for very low NP concentrations as compared with a commercial dye Hoechst ( Figure 8B).…”

“…VITDDK3 90 Cubic [50] Fungi Ganoderma spp. 20 Spherical [19] Green algae Prasiola crispa 9.8 Face-centred cubic [51] Biomolecule Ascorbic acid 20-25 Spherical [52] Nickel Plant Aegle marmelos Correa 80-100 Face centred cubic [53] Copper Plant Aegle marmelos Correa 50-100 Face centred cubic [54] Biomolecule Ascorbic acid and chitosan 100 Nd [55] Lead Plant Jatropha curcas 10-12.5 Spherical [56] Palladium Plant Coffee and tea 20-60 Face centred cubic [57] Selenium Bacteria Bacillus subtilis 50-400 Spherical [58] P3HB Bacteria Bacillus firmus 43.7 Nd [59] Magnetosome Bacteria Magnetospirillum gryphiswaldense 64-78 Spherical [60] Zinc oxide Plant Ocimum basilicum 50 Hexagonal [61] Bacteria Lactobacillus plantarum 7-19 Roughly spherical [62] Marine algae Sargassum muticum 30-57 Hexagonal wurtzite [63] Iron oxide Bacteria M. gryphiswaldense 24 Spherical [64] Biomolecule Starch 30-40 Spherical [31] Cadmium telluride Bacteria Escherichia coli 2.0-3.2 Spherical [65] Earthworm Lumbricus rubellus 2.3 Nearly spherical [66] Cadmium sulfide Plant Trigonella foenum graecum 53 Triangle [67] Lead selenide Marine microbe Aspergillus terreus 59 Rod [68] Silver chloride Plant Cissus quadrangularis 15-23 Spherical [69] Silver-gold Protein Macerase enzyme 4-20 Spherical [70] Plant Indian Rosewood 15-25 Spherical [71] Iron oxide-silver Plant Vitis vinifera 50 Spherical Reproduced with permission from [32]. © Elsevier (2013).…”