Green synthesis of high yield mono-dispersed gold nanoparticles using silk-sericin and characterization

Parushuram, N.; Asha, S.; Suma, S.; Krishna, K.; Neelakandan, R.; Sangappa, Y.

doi:10.1063/1.5122565

Cited by 6 publications

(2 citation statements)

References 10 publications

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“…Then, the cocoon pieces were chemically degummed by boiling two times with 0.02 M Na 2 CO 3 solution at 95 °C for exactly 30 min. Subsequently, the sericin-free SF fiber was washed and cooled by rinsing with double distilled water and then dried. − The degummed SF fiber was dissolved in 9.3 M aqueous LiBr solution at 60 °C for ∼4 h. The obtained SF solution was then cooled and filled in a dialysis cassette with MWCO 3500 Da (Slide-A-Lyzer: Thermo Fisher Scientific), followed by dialysis against de-ionized water for 3 days at room temperature to eliminate the residual salts. The dialyzed clear SF solution was centrifuged for 20 min at 9000 rpm to eliminate the silk accumulation, if any had occurred during the process.…”

Section: Methodsmentioning

confidence: 99%

Conductive In Situ Reduced Graphene Oxide–Silk Fibroin Bionanocomposites

et al. 2021

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This research paper describes the fabrication of bionanocomposites (BNCs) based on silk fibroin (SF) and reduced graphene oxide (rGO). The recorded UV–visible (UV–vis) spectra of the sample confirm the reduction of GO to rGO in SF by showing a plasmon resonance band within the wavelength range of 261–268 nm. The X-ray diffraction (XRD) peak at 11.6° corresponding to the GO intensity decreases with increasing reaction time, resulting in rGO in the SF host matrix. The morphological behavior of the SF–rGO BNCs is scrutinized using scanning electron microscopy (SEM), and the images clearly indicate the existence of rGO within the matrix. The increasing amount of GO in the SF shows broken graphene sheets, which can increase the surface roughness and establish a strong physical contact between the SF and rGO nanosheets. The high-resolution transmission electron microscope (HR-TEM) image of the bionanocomposite showed that the formed rGO encompassments of fewer layers are stacked, each with fewer wrinkles and folding. The Raman spectroscopy confirmed the formation of rGO by showing the increased intensity ratio of D to G band (I D/I G) in the bionanocomposite samples. The rGO effect on the electrical conductivity is measured, and the results show that DC conductivity increases from 1.28 × 10–9 to 82.4 × 10–9 S/cm with an increase in the GO content in the SF biopolymer. The investigations demonstrate loss of the insulation property and improved conducting behavior of the SF biopolymer.

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Section: Methodsmentioning

confidence: 99%

Conductive In Situ Reduced Graphene Oxide–Silk Fibroin Bionanocomposites

et al. 2021

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“…3,8,9 Mostly like other metal nanoparticles, the gold nanoparticle biosynthesis by the use of green methods using a natural polymer as the reducing and capping agent has attracted massive attention in recent years, as it is technologically important in various fields like tissue engineering, electronic, biosensing, anti-bacterial, drug delivery and cancer therapy, etc. 3,7,8,[10][11][12] The cocoons of Bombyx mori are natural silk compounds having multifunctional properties with exclusive structure. The B. mori cocoons are the main source of silk products and are composed of an inner shell (fibroin, 70%) and a covered outer shell (sericin, 30%).…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Biogenic Gold Nanoparticles by Using Sericin Protein from Bombyx mori Silk Cocoon and Investigation of Its Wound Healing, Antioxidant, and Antibacterial Potentials

Das¹,

Seo²,

Yang³

et al. 2023

IJN

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Introduction A number of biological wastes and factory waste materials have been tested recently for the eco-friendly biosynthesis of nanoparticles. Sericin protein (SSP) is usually removed from the silk cocoon during the degumming process in the process of making the silk, and this sericin protein is normally thrown away by the sericulture industries as waste materials. It is found that this sericin protein possesses a number of biological properties. Methods Considering this, in the present study, an effort has been made to biosynthesize gold nanoparticles (SSP-AuNPs) using the waste sericin solution as the reducing and capping agent and investigate its biopotential in terms of its wound healing, antioxidant and antibacterial activities. Results The synthesis of SSP-AuNPs was perceived by the visual color change and confirmed by UV-Vis spectroscopy with absorption maxima at 522 nm. Further characterization of SSP-AuNPs was done by TEM, EDS, XRD, FTIR, DLS, zeta potential, TGA, AFM, etc. The size of SSP-AuNPs was found out to be 54.82 nm as per the particle size analyzer and the zeta potential is −19.8 mV. The SSP-AuNPs displayed promising wound healing potential of 70.96 and 69.76% wound closure rate at 5 and 10 µg/mL respectively as compared to 74.91% by the Centella asiatica taken as a positive control. It also exhibited promising antioxidant potential in terms of the DPPH, ABTS free radical scavenging, reducing power potential, and total antioxidant capacity. Besides, the SSP-AuNPs also displayed significant antibacterial activities against the tested pathogenic bacterial with the diameter of inhibition zones ranging between 12.10 and 14.96 mm as compared to the positive control cephalexin that displayed inhibition zones ranging between 12.08 and 13.24 mm. Discussion Taken together, SSP-AuNPs could serve as an interesting candidate for food, cosmetics, and biomedical fields in the applications of wound healing, cosmetics, antibacterial bandages, and ointments, etc.

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Silk protein-based nanoparticles for therapeutic applications

Parashar¹,

Pathak²

2023

Polymeric Nanosystems

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Green synthesis of high yield mono-dispersed gold nanoparticles using silk-sericin and characterization

Cited by 6 publications

References 10 publications

Conductive In Situ Reduced Graphene Oxide–Silk Fibroin Bionanocomposites

Conductive In Situ Reduced Graphene Oxide–Silk Fibroin Bionanocomposites

Synthesis of Biogenic Gold Nanoparticles by Using Sericin Protein from Bombyx mori Silk Cocoon and Investigation of Its Wound Healing, Antioxidant, and Antibacterial Potentials

Silk protein-based nanoparticles for therapeutic applications

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