Green synthesis of highly stabilized nanocrystalline silver particles by a non-pathogenic and agriculturally important fungusT. asperellum

Abstract

A controlled and up-scalable biosynthetic route to nanocrystalline silver particles with well-defined morphology using cell-free aqueous filtrate of a non-pathogenic and commercially viable biocontrol agent Trichoderma asperellum is being reported for the first time. A transparent solution of the cell-free filtrate of Trichoderma asperellum containing 1 mM AgNO(3) turns progressively dark brown within 5 d of incubation at 25 °C. The kinetics of the reaction was studied using UV-vis spectroscopy. An intense surface plasmon resonance band at ∼410 nm in the UV-vis spectrum clearly reveals the formation of silver nanoparticles. The size of the silver particles using TEM and XRD studies is found to be in the range 13-18 nm. These nanoparticles are found to be highly stable and even after prolonged storage for over 6 months they do not show significant aggregation. A plausible mechanism behind the formation of silver nanoparticles and their stabilization via capping has been investigated using FTIR and surface-enhanced resonance Raman spectroscopy.

Eco-friendly drugs from the marine environment: spongeweed-synthesized silver nanoparticles are highly effective on Plasmodium falciparum and its vector Anopheles stephensi, with little non-target effects on predatory copepods

“…3). The broad intense band close to 3402/cm may be assigned to the N-H stretching frequency arising from peptide linkages (Mukherjee et al 2008). The presence of peak at 3416/cm could be ascribed to O-H group in polyphenols or proteins/ enzymes or polysaccharides (Susanto et al 2009).…”

Bio-Nanotechnology for Active Food Packaging

“…The assimilation of different nanoparticles such as SiO 2 , clay, TiO 2 , KMnO 4 , nano cellulose, nano fibrillated cellulose and carbon nanotubes are capable of amending the packaging material in terms of mechanical and barrier properties (Tsai et al, 2010;Reddy et. al., 2007;Xie et al, 2012;Hejri et al, 2013;Saeidnia et al, 2013;Mukherjee et al, 2014;Yang and Yao, 2014). It has been reported that packaging material mechanical properties such as ductile strength, shear strength, barrier potential, thermal constancy and optical characteristics of polymers can be enhanced by assimilating nano materials into their matrices (Zhong et al, 2007).…”

“…Edible coating is done with the help of liquid film forming solution onto the surface of the food products. They are created by casting or by traditional plastic extrusion techniques (Park et al, 1996).…”

“…Biotechnologically synthesized nanoparticles are on the path of greener and ecofriendly environment. Biogenic synthesis approach used for the production of nanoparticles showed non-toxic affects as compared to the chemical synthesis approach which involves the toxic chemicals as well as require optimum conditions for its processing (Tyagi et al, 2015;Gade et al, 2008;Mukherjee et al, 2008). Bio-nanotechnology is firmly being used in the areas like food production and packaging (Sharma and Saharan et al, 2016;Saharan et al, 2013;Duncan et al, 2011;Sorrentino et al, 2007;Kuzma et al, 2008;Sanguansri and Augustin, 2006).…”

Synthesis of gold nanoparticles of variable morphologies using aqueous leaf extracts ofCocculus hirsutus

“…So the interests of researchers have turned towards green synthesis. The metal-microbe interactions have an important role in several biotechnological applications, and microorganisms are considered as potential biofactory for the synthesis of nanoparticles [22][23][24][25][26][27]. Gold nanoparticles are considered biocompatible but chemical synthesis methods may still lead to the presence of some toxic chemical species absorbed on the surface that may have adverse effects in medical applications [28].…”