Green synthesis of magnetic Fe₃O₄ nanoparticles using Couroupita guianensis Aubl. fruit extract for their antibacterial and cytotoxicity activities

Abstract

In the present study, a sustainable green chemistry approach was established to fabricate magnetic FeO nanoparticles (FeONPs) using the aqueous fruit extract of edible C. guianensis (CGFE). Synthesized NPs were further confirmed with different high-throughput characterization techniques such as UV-visible spectroscopy, FT-IR, XPS, DLS and zeta potential analysis. Additionally, XRD, AFM, HRTEM and SQUID VSM demonstrate the generation of crystalline CGFeONPs with mean diameter of 17 ± 10 nm. Interestingly, CGFeONPs exhibit a stupendous bactericidal action against different human pathogens which depicts its antimicrobial value. A significant dose-dependent cytotoxic effect of CGFeONPs was noticed against treated human hepatocellular carcinoma cells (HepG2).

Ultrasound assisted-phytofabricated Fe 3 O 4 NPs with antioxidant properties and antibacterial effects on growth, biofilm formation, and spreading ability of multidrug resistant bacteria

“…Various dimensions of images ( Figure 5(a-f)) were utilized to detect clearly NPs. As illustrated in Figure 5 showed size distribution range 7-77 nm for green synthesized magnetic Fe 3 O 4 NPs by fruit extract of Couroupita guianensis Aubl [62]. Therefore, as a comparative approach, our results showed reduction and uniformity of Fe 3 O 4 NPs size.…”

“…As illustrated in Figure 5(d), lower height of 2.73 nm and uniformity of size distribution were observed for Fe 3 O 4 NPs. In this case, the previous study showed size distribution range 7-77 nm for green synthesized magnetic Fe 3 O 4 NPs by fruit extract of Couroupita guianensis Aubl [62].…”

“…Green synthesized Fe 3 O 4 NPs by fruit extract of Couroupita guianensis Aubl. had more antibacterial activity on gram-negative bacteria K. penumoniae MTCC 530, E. coli MTCC 2939, and S. typhi MTCC 3917 than gram-positive bacterium S. aureus MTCC 96 [62]. In this case, the generation of reactive oxygen species (ROS) as an antibacterial factor may result from MNPs unique properties.…”

An updated review on Couroupita guianensis Aubl: a sacred plant of India with myriad medicinal properties

“…Further, CGFE and CGFe 3 O 4 NPs treated HepG2 cells showed modified features like condensed nuclei, apoptotic bodies of nuclear morphology and membrane blebbing. These results made the authors to strongly suggest that CGFe 3 O 4 NPs induces HepG2 cell death through caspase-cascade mediated mitochondrial dysfunction and apoptosis (66). A similar MTT assay was carried out by the same author with palladium nanoparticles (CGPdNPs) obtained from CG fruit extract (CGFE) on lung cancer cell line A549.…”

Comparison of anticancer activity of biocompatible ZnO nanoparticles prepared by solution combustion synthesis using aqueous leaf extracts of Abutilon indicum, Melia azedarach and Indigofera tinctoria as biofuels

“…In addition to all this biosynthesized NPs are usually biocompatible and highly applicable for biomedical applications [16,17,18]. Green synthesis of different NPs by plants such as Acalypha indica, Rosa hybrid, Marraya koenigii, Tanacetum vulgar, Punica granatum, Tamarindus indica, Prunus japonica, Couroupita guianensis, Borago officinalis, etc., has been reported [23][24][25][26][27][28]. This begs a question; whether the use of naturally occurring phytochemicals pose any strategic advantage over using chemically purified counterparts, other than just being cheaper or naturally available?…”