Green synthesis of silver/montmorillonite/chitosan bionanocomposites using the UV irradiation method and evaluation of antibacterial activity

Abstract

Abstract:In this study, silver nanoparticles (Ag-NPs) were synthesized using a green physical synthetic route into the lamellar space of montmorillonite (MMT)/chitosan (Cts) utilizing the ultraviolet (UV) irradiation reduction method in the absence of any reducing agent or heat treatment. Cts, MMT, and AgNO 3 were used as the natural polymeric stabilizer, solid support, and silver precursor, respectively. The properties of Ag/MMT/Cts bionanocomposites (BNCs) were studied as the function of UV irradiation times. UV irradiation disintegrated the Ag-NPs into smaller sizes until a relatively stable size and size distribution were achieved. Meanwhile, the crystalline structure and d-spacing of the MMT interlayer, average size and size distribution, surface morphology, elemental signal peaks, functional groups, and surface plasmon resonance of Ag/MMT/Cts BNCs were determined by powder X-ray diffraction, transmission electron microscopy, scanning electron microscopy, energy dispersive X-ray fluorescence, Fourier transform infrared, and UV-visible spectroscopy. The antibacterial activity of Ag-NPs in MMT/Cts was investigated against Gram-positive bacteria, ie, Staphylococcus aureus and methicillin-resistant S. aureus and Gram-negative bacteria (ie, Escherichia coli) by the disk diffusion method on Muller-Hinton Agar at different sizes of Ag-NPs. All of the synthesized Ag/MMT/Cts BNCs were found to have high antibacterial activity. These results show that Ag/ MMT/Cts BNCs can be useful in different biologic research and biomedical applications, such as surgical devices and drug delivery vehicles.

Synthesis of silver nanoparticles in montmorillonite and their antibacterial behavior

“…Based on our previous work, therefore, Ag NPs in the interlayer space of MMT were synthesized according to two different methods, the chemical and physical reduction techniques, using β-D-Glucose, 33 NaBH 4 , 34,35 UV, [36][37][38] and γ-irradiation 39 methods as the reducing agents. In this study, the different-sized Ag NPs were synthesized in the lamellar space of the MMT in the aqueous solution, by changing the initial concentration of Ag + ions at room temperature.…”

Synthesis, characterization, and antimicrobial properties of copper nanoparticles

“…23 An additional confirmatory test was performed by studying the molecular interaction between the chitosan medium and the synthesized nanoparticles using FT-IR. 41 For spectra b-e in Figure 3, blue shifts and decreased intensity of peaks were noticed, eg, a blue shift of the N−H bending peak at 1,606 cm −1 to 1,601 cm…”

Green biosynthesis of silver nanoparticles using Curcuma longa tuber powder

“…There are several ways to reduce Ag + , eg, use of γ-rays, 4 ultraviolet (UV) irradiation, 5 heating and electrochemical reduction, 6 and application of reducing chemicals, such as hydrazine, 7 sodium borohydride, [8][9][10] polyethylene glycerol, 11 N,N-dimethylformamide, 12 glucose, 13 ethylene glycol, 14 formaldehyde, 15 and sodium in liquid ammonia. 16 However, there is still need for a more economic, commercially viable, and environmentally green synthesis route to synthesize Ag-NPs.…”