Green Synthesis of Silver Nanoparticles from Camellia sinensis and Its Antimicrobial and Antibiofilm Effect against Clinical Isolates

Abstract

The green synthesis method of was used for the synthesis of silver nanoparticles using Camellia sinensis (green tea). The Camellia sinensis silver nanoparticles (CS-AgNPs) were characterized using different techniques, including UV-Vis (ultra violet-visible), SEM (scanning electron microscopy), TEM (transmission electron microscopy), and XRD (X-ray diffraction). The average size of the CS-AgNPs was 52 nm, according to TEM. The CS-AgNPs showed excellent antibacterial and antifungal activity. The MIC (minimum inhibitory concentration) against bacterial isolates varied from 31.25 to 62.5 µg/mL, whereas for fungal isolates, the MIC varied from 125 to 250 µg/mL. The presence of a zone in the well diffusion assay showed the antimicrobial nature of CS-AgNPs. Further, CLSM (confocal laser scanning microscopy) showed that CS-AgNPs possess antibiofilm activity. The interaction of CS-AgNPs with the Candidal cells was analyzed using TEM, and it was revealed that CS-AgNPs entered the cell and disrupted the cell machinery.

Eco-friendly Approach for Silver Nanoparticles Synthesis from Lemon Extract and their Anti-oxidant, Anti-bacterial, and Anti-cancer Activities

“…Compared with the previous studies, the silver nanoparticles in this work were similar in shape and size to those obtained in studies (16,34). In comparison, it was smaller than AgNPs obtained in studies (35)(36)(37).…”

Complexes of Ag and ZnO nanoparticles with BBR for enhancement of gastrointestinal antibacterial activity through the impacts of size and composition

“…17,18 It was reported that the minimum inhibitory concentration of biosynthesized Ag and ZnO NPs against various bacterial strains varied in the ranges of 31.25 O 62.5 mg L −1 and 440 O 854 mg L −1 , respectively. 19,20 Although the antibacterial activity of ZnO NPs is not superior to that of other metal materials, the worthy property of ZnO NPs is their good biocompatibility with human cells. 21 Variations in structure, size, and composition of nanomaterials greatly improve their performance in different applications, 22,23 as well as modulate their impact on health and the environment.…”

Synthesis of Silver Nanoparticles Using Camellia sinensis Leaf Extract: Promising Particles for the Treatment of Cancer and Diabetes

“…Conversely, Ali et al (2022) synthesized AgNPs using hot water for the preparation of C. sinensis leaf extract. [35] Once nanoparticles are synthesized, it is necessary to confirm and characterize them using various methods. The change in color from pale green to dark brown primarily indicates the synthesis of silver nanoparticles.…”