Green Synthesis of Silver Nanoparticles Using Pseudoduganella eburnea MAHUQ-39 and Their Antimicrobial Mechanisms Investigation against Drug Resistant Human Pathogens

Abstract

Silver nanoparticles (AgNPs) have shown great promise in biomedical applications. The exact mechanism and mode of action of AgNPs regarding antimicrobial activity are still not well known. Moreover, synthesis of nanoparticles by physical and chemical methods is expensive and not ecofriendly. This study highlights the green, rapid, facile, cost-effective and ecofriendly synthesis of AgNPs using Pseudoduganella eburnea MAHUQ-39 and also investigates their antibacterial mechanisms. The transmission electron microscopy (TEM) image revealed a spherical shape of the AgNPs. The size of the synthesized AgNPs was 8 to 24 nm. The elemental mapping and selected area electron diffraction (SAED) and X-ray diffraction (XRD) patterns revealed the crystalline structure of AgNPs. Fourier-transform infrared spectroscopy (FTIR) analysis identified the functional groups that are involved in the reduction of silver ion to AgNPs. The green synthesized AgNPs exhibited strong antimicrobial activity against multidrug-resistant pathogenic microbes. Minimal inhibitory concentrations (MICs) of Staphylococcus aureus and Pseudomonas aeruginosa were 100 μg/mL and 6.25 μg/mL, respectively, and the minimum bactericidal concentrations (MBCs) of S. aureus and P. aeruginosa were 200 μg/mL and 50 μg/mL, respectively. Our data demonstrated that synthesized AgNPs created structural changes of cells and destroyed the membrane integrity of strains S. aureus and P. aeruginosa. Therefore, AgNPs synthesized by strain MAHUQ-39 can be used as a powerful antimicrobial agent for various therapeutic applications.

Three-Dimensional Printed Antimicrobial Objects of Polylactic Acid (PLA)-Silver Nanoparticle Nanocomposite Filaments Produced by an In-Situ Reduction Reactive Melt Mixing Process

“…The results obtained in our study regarding the differences in bacteria viability between Gram-positive and Gram-negative strains using AgNPs as the antimicrobial agent, are in good agreement with findings recently reported by Huq et al [62]. Specifically, in this work the growth curves of Gram-positive S. aureus Gram-negative E. coli bacteria strains cultured in R2A broth with various concentrations of AgNPs have been reported, showing a higher AgNP susceptibility of Gram-negative bacteria compared to that of Gram-positive bacteria.…”

Three-Dimensional Printed Polylactic Acid (PLA) Surgical Retractors with Sonochemically Immobilized Silver Nanoparticles: The Next Generation of Low-Cost Antimicrobial Surgery Equipment

“…Overall, the sonochemically prepared PLA@Ag retractor exhibits excellent antibacterial properties with the greatest bactericidal effect on E.coli (98.48%) and the lowest on S. aureus (63.05%) at 60 min (p < 0.05). The differences found in bacteria viability between Gram-positive and Gram-negative strains is in good agreement with findings from a recent study by Huq et al [52]. Specifically, in this work, the growth curves of P. aeruginosa and S. aureus cultured in R2A broth with various concentrations of AgNPs have been shown, demonstrating a higher AgNPs susceptibility of Gram-negative bacteria compared to that of Gram-positive bacteria.…”

Phyto-Mediated Synthesis of Silver Nanoparticles Using Terminalia chebula Fruit Extract and Evaluation of Its Cytotoxic and Antimicrobial Potential

“…Prominent efforts have been made in the synthesis of metal nanoparticles using plants and microorganisms. Silver nanoparticles are profoundly important, and used as antimicrobial agents [1], in water purification systems [2] and in cosmetics [3]. Moreover, AgNPs are very much suitable for biological imaging and sensing as they exhibit strong optical properties [4].…”