Green synthesis of silver nanoparticles and characterization of their inhibitory effects on AGEs formation using biophysical techniques

Abstract

Advanced glycation end-products (AGEs) resulting from non-enzymatic glycation are one of the major factors implicated in secondary complications of diabetes. Scientists are focusing on discovering new compounds that may be used as potential AGEs inhibitors without affecting the normal structure and function of biomolecules. A number of natural and synthetic compounds have been proposed as AGE inhibitors. In this study, we investigated the inhibitory effects of AgNPs (silver nanoparticles) in AGEs formation. AgNPs (~30.5 nm) synthesized from Aloe Vera leaf extract were characterized using UV-Vis spectroscopy, energy-dispersive X-ray spectroscopy (EDX), high resolution-transmission electron microscopy, X-ray diffraction and dynamic light scattering (DLS) techniques. The inhibitory effects of AgNPs on AGEs formation were evaluated by investigating the degree of reactivity of free amino groups (lysine and arginine residues), protein-bound carbonyl and carboxymethyl lysine (CML) content, and the effects on protein structure using various physicochemical techniques. The results showed that AgNPs significantly inhibit AGEs formation in a concentration dependent manner and that AgNPs have a positive effect on protein structure. These findings strongly suggest that AgNPs may play a therapeutic role in diabetes-related complications.

Facile biosynthesis of Ag-NPs using Otostegia limbata plant extract: Physical characterization and auspicious biological activities

“…Nowadays, fabrication of Ag-NPs by using plant extracts is getting more popular due to its eco-friendly and bio-compatible nature. [20][21][22] Krishnaraj et al synthesized Ag-NPs by using Acalypha indica plant extract, 23 similarly in present study O. limbata plant extract was adopted for the production of Ag-NPs. Water based AgNO 3 solution was morphed into brownish color within 20 minutes, due to the addition of plant extract (Fig.…”

“…4) 04-0783. The average crystallite size of the Ag-NPs engineered via bioprocess was determined by using Scherrer formula 22 [D = 0.9λ/βcosθ] which is approximately 8 nm. These findings represent that 32 nm of particle size is surely made up of 8 nm of multiple crystallite units.…”

Potential of biosynthesized silver nanoparticles using Stenotrophomonas sp. BHU-S7 (MTCC 5978) for management of soil-borne and foliar phytopathogens

“…The Fourier transform infrared (FTIR) spectrum for analyzing functional nature of AgNPs was recorded on Perkin Elmer FT-IR spectrometer Spectrum Two (Perkin Elmer Life and Analytical Sciences, CT, USA) using potassium bromide (KBr) pellets in the ratio of 1:100. The spectrum was recorded in the diffuse reflectance mode at a resolution of 4 cm −1 in the range of 400–4000 cm −1 wavenumber42. For elemental composition, energy dispersive X-ray (EDAX) spectrum was collected using EDAX detector (Oxford Instruments INCAx-sight EDAX spectrometer) equipped with SEM.…”

Nano Dimensional (1-20nm) Silver Nanoparticles: Stem Extract of Capparis decidua (FORSK.) EDGEW Mediated Synthesis and its Characterization-A Lab to Land Approach

“…In comparison to microorganisms, the application of plant extracts for the synthesis of AgNPs is more advantageous in terms of resource availability, security, reaction rate and convenience, and feasibility of large scale synthesis (Mane Gavde et al, 2015, Jalaluddin et al, 2016. The particle size, growth can also be controlled by altering synthesis conditions like pH, reductant concentration, temperature, mixing ratio of the reactants etc.…”