Green Synthesis of Silver Nanoparticles Using Extract of Cilembu Sweet Potatoes (Ipomoea batatas L var. Rancing) as Potential Filler for 3D Printed Electroactive and Anti-Infection Scaffolds

Abstract

Electroactive biomaterials are fascinating for tissue engineering applications because of their ability to deliver electrical stimulation directly to cells, tissue, and organs. One particularly attractive conductive filler for electroactive biomaterials is silver nanoparticles (AgNPs) because of their high conductivity, antibacterial activity, and ability to promote bone healing. However, production of AgNPs involves a toxic reducing agent which would inhibit biological scaffold performance. This work explores facile and green synthesis of AgNPs using extract of Cilembu sweet potato and studies the effect of baking and precursor concentrations (1, 10 and 100 mM) on AgNPs’ properties. Transmission electron microscope (TEM) results revealed that the smallest particle size of AgNPs (9.95 ± 3.69 nm) with nodular morphology was obtained by utilization of baked extract and ten mM AgNO3. Polycaprolactone (PCL)/AgNPs scaffolds exhibited several enhancements compared to PCL scaffolds. Compressive strength was six times greater (3.88 ± 0.42 MPa), more hydrophilic (contact angle of 76.8 ± 1.7°), conductive (2.3 ± 0.5 × 10−3 S/cm) and exhibited anti-bacterial properties against Staphylococcus aureus ATCC3658 (99.5% reduction of surviving bacteria). Despite the promising results, further investigation on biological assessment is required to obtain comprehensive study of this scaffold. This green synthesis approach together with the use of 3D printing opens a new route to manufacture AgNPs-based electroactive with improved anti-bacterial properties without utilization of any toxic organic solvents.

Green Synthesized of Ag/Ag2O Nanoparticles Using Aqueous Leaves Extracts of Phoenix dactylifera L. and Their Azo Dye Photodegradation

“…It is a relatively new scientific field that adopts engineering nanoparticles ranging from metals, metal oxides, and hybrids [3][4][5]. This approach is biologically safe, non-toxic, and environmentally friendly, as natural plant extracts are typically used [6,7]. The biomolecules present in the plant extract can reduce metal ions to nanoparticles (NPs) in a single-step green synthesis process [6,8].…”

Characterization and Antimicrobial Activity of Silver Nanoparticles Synthesized with the Peel Extract of Mango

“…Therefore, the aggregation was ascribed to the layer (capping agents) covering the AgNPs, causing them to adhere to each other [ 53 ]. The particle sizes of the AgNPs increased in conjunction with higher mango peel extract concentrations and shape changes, which was related to the crystal nucleus growth rate [ 54 ]. A lower mango peel extract concentration decelerated the AgNP nucleation rate, allowing more significant nucleation to occur simultaneously.…”

Systematic Evaluation of Antioxidant Efficiency and Antibacterial Mechanism of Bitter Gourd Extract Stabilized Silver Nanoparticles

“…The surface charge on the AgNPs in aqueous media was determined by measuring the Zeta potential (Figure 2c,d The crystalline structures of A-BGE and E-BGE synthesized AgNPs were elucidated by the XRD diffraction patterns. As shown in Figure 3, the diffraction peaks of the AgNPs at 27.8 • , 32.3 • , 46.2 • , 54.9 • , and 57.7 • were indexed to the (111), ( 200), ( 220), (311), and (222) reflection plane corresponding to the AgCl (PDF-31-1238), respectively, due to the crystallization of the bio-organic phase in the extract for both A-BG-AgNPs and E-BG-AgNPs [45]. Whereas, some extra diffraction peaks were observed only for A-BG-AgNPs at 38.3 • , 44.2 • , 64.6 • , and 77.5 • , which were indexed to the (111), ( 200), ( 220), (311) crystal planes of Ag (PDF- .…”