Green synthesis of silver nanoparticles: characterization and determination of antibacterial potency

Abstract

Silver ions (Ag+) and its compounds are highly toxic to microorganisms, exhibiting strong biocidal effects on many species of bacteria but have a low toxicity toward animal cells. In the present study, silver nanoparticles (SNPs) were biosynthesized using aqueous extract of Chlorella vulgaris as reducing agent and size of SNPs synthesized ranged between 15 and 47 nm. SNPs were characterized by UV–visible spectroscopy, scanning electron microscopy, transmission electron microscopy, X-ray diffraction and Fourier infrared spectroscopy, and analyzed for its antibacterial property against human pathogens. This approach of SNPs synthesis involving green chemistry process can be considered for the large-scale production of SNPs and in the development of biomedicines.

Eco-Friendly Synthesis and Antimicrobial Activity of Silver Nanoparticles Using Dracocephalum moldavica Seed Extract

“…Yallapa et al reported similar results in previous reports for AgNPs by using A. farnesiana plant extracts [37], and Song and Kim and Raghunadan et al discovered C and O in MNPs obtained using pine, persimmon, magnolia, ginkgo, platanus, and guava extracts [3,40,41]. The antimicrobial and antibiotics resistance of human pathogens made as problematic issue which needs to discover new natural alternates to overcome this problem [42,43]. The antibacterial activity of synthesized AgNPs was tested against six bacteria: S. aureus, S. epidermidis, B. subtilis as Gram positive bacteria, E. coli, P. aeruginosa, and S. marcescens as Gram negative bacteria.…”

Nannochloropsis Extract–Mediated Synthesis of Biogenic Silver Nanoparticles, Characterization and In Vitro Assessment of Antimicrobial, Antioxidant and Cytotoxic Activities

“…Thus, FTIR analysis clearly specified that the carboxyl groups of fatty acids, tetraterpenoids of xanthophylls, carboxyl and hydroxyl groups of polyphenols, carbonyl and amide linkage of proteins might be actively involved in the formation of biosynthesized AgNPs. In addition, related work involved hydroxyl and amino protein groups in the biosynthesis of AgNPs from the Chlorella vulgaris microalgae extract (Annamalai and Nallamuthu, 2016). GCMS analysis concludes that the phytochemicals like octadecanoic acid, hexadecanoic acid, oxygenated tetraterpenoids and phenolic acids present in EAENH fraction were accountable for bioreduction and stabilization of AgNPs.…”

Synthesis and characterization of nontoxic silver nano-particles with preferential bactericidal activity

“…The band at 1635 cm -1 is attributed to C=C [33]. The absorption peak at 3432 cm -1 is assigned to N-H stretching vibration [34], whereas the band at 2925 cm -1 is associated with the C-H stretching vibration-asymmetric [35,36]. The peak at 1385 cm -1 is corresponding to C-H symmetric deformation vibration [37] while the one at 1033 cm -1 is assigned to C-O stretching [38].…”