Green synthesis of silver nanoparticles using Mentha pulegium and investigation of their antibacterial, antifungal and anticancer activity

Abstract

A simple and eco-friendly method for efficient synthesis of stable colloidal silver nanoparticles (AgNPs) using extracts is described. A series of reactions was conducted using different types and concentrations of plant extract as well as metal ions to optimize the reaction conditions. AgNPs were characterized by using UV-vis spectroscopy, transmission electron microscopy, atomic force microscopy, dynamic light scattering, zetasizer, energy-dispersive X-ray spectroscopy (EDAX) and Fourier transform infrared spectroscopy (FTIR). At the optimized conditions, plate shaped AgNPs with zeta potential value of -15.7 and plasmon absorption maximum at 450 nm were obtained using high concentration of aqueous extract. Efficient adsorption of organic compounds on the nanoparticles was confirmed by FTIR and EDAX. The biogenic AgNPs displayed promising antibacterial activity on, , and. The highest antibacterial activity of 25 µg mL-1 was obtained for all the strains using aqueous extract synthesized AgNPs. The aqueous extract synthesised AgNPs also showed considerable antifungal activity against fluconazole resistant . The cytotoxicity assay revealed considerable anticancer activity of AgNPs on HeLa and MCF-7 cancer cells. Overall results indicated high potential of extract to synthesis high quality AgNPs for biomedical applications.

Therapeutic prospective of plant-induced silver nanoparticles: application as antimicrobial and anticancer agent

“…AgNPs synthesized from the leaf extract of Mentha pulegium were found cytotoxic to Hela and MCF-7 cancer cells [10]. AgNPs were treated on MCF-7 cell line shows drastic changes in morphological characteristics.…”

“…Along with that cytoplasmic shrinkage and cell clustering was observed unlike the case in AgNPs treated normal cells. The cells were treated with 20 lg/mL concentration of AgNPs and kept for 48 h for further observation and found apoptosis of the cells [10]. AgNPs synthesized with Coriandrum sativum and Prosopis cineraria extract as a reducing agent showed anticancer activity against MCF-7 cancer cells [11,14].…”

“…Its antibacterial and toxicity were shown by zone of inhibition [17]. Biogenic colloidal AgNPs synthesized with Mentha pulegium extract as a reducing agent showed antibacterial activity against Escherichia coli, Staphylococcus aureus, Steptococcus pyogenes, Candida Albicans [10]. AgNPs synthesized from Syzygium jambos showed antibacterial and antifungal properties [37].…”

<p>Comparison of cytocompatibility and anticancer properties of traditional and green chemistry-synthesized tellurium nanowires</p>

“…46 With such a promise shown in previous studies, there has been an increased interest in the generation of green NPs that also have anticancer properties. For example, copper oxide NPs made by Rehana et al 47 and silver NPs produced by Kelkawi et al 48 that were both synthesized using different plant extracts showed attractive anticancer properties.…”

The use of PWHM and Mie methods in estimation of colloidal silver particle size obtained by chemical precipitation with sodium borohydride

“…Green synthesis methods are increasingly being investigated, using natural compounds such as mint fragrant [10], honey [11], white sugar [12], Arab rubber [13], corn dextrose [14], silkworm sericin [15] and glucose [16] as reducers. By synthesis using a spinning disk reactor with glucose as a reducing agent and starch, as a cheap and non-toxic stabilizer, nanoparticles up to 10 nm in diameter were obtained and remained stable over 40 days [17].…”