Green Synthesis of Silver Nanoparticles using &lt;i&gt;Azadirachta Indica&lt;/i&gt; and &lt;i&gt;Ocimum Sanctum&lt;/i&gt; Leaf Extract

Priyadarshini, Sonali; Sulava, Sushree; Bhol, Rasmita; Jena, Srikanta

doi:10.18520/cs/v117/i8/1300-1307

Cited by 26 publications

(14 citation statements)

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“…Aqueous extract of pomegranate (P. granatum) leads to AgNP synthesis in 2 h (Khorrami et al 2019). Methanolic extracts of A. indica L. leaves fabricated AgNPs in 2 h while Punica granatum L. fruit aqueous extract showed AgNPs synthesis in 10 min under sunlight (Priyadarshini et al 2019;Mohseni et al 2020). Ai-AgNPs and Pg-AgNPs displayed characteristic LSPR peaks at 434 nm and 416 nm, respectively (Fig.…”

Section: Green Synthesis Of Agnpsmentioning

confidence: 97%

Characterization of biogenically synthesized silver nanoparticles for therapeutic applications and enzyme nanocomplex generation

2020

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The present study describes green synthesis of silver nanoparticles (AgNPs) and inulin hydrolyzing enzyme nanocomplexes (ENC) using Azadirachta indica (Ai) and Punica granatum (Pg) leaf extracts. Surface topology and physico-chemical characteristics of AgNPs were studied using surface plasmon resonance (SPR), FTIR, SEM, AFM and EDX analyses. Particle size analysis using dynamic light scattering and AFM studies revealed that Ai-AgNPs (76.4 nm) were spherical in shape having central bigger nano-regime with smaller surroundings while Pg-AgNPs (72.1 nm) and ENCs (Inulinase-Pg-AgNPs ~ 145 nm) were spherical particles having smooth surfaces. Pg-AgNPs exhibited significant photocatalysis of a thiazine dye, methylene blue. Both Ai-and Pg-AgNPs showed selective antibacterial action by inhibiting pathogenic Bacillus cereus, while the probiotic Lactobacillus strains remained unaffected. Ai-AgNPs showed potential anti-biofilm effect (30% viability) on B. cereus biofilms. Pg-AgNPs showed anti-cancer effect against human colon cancer cell lines (Caco-2) resulting in 40% cell death in 48 h. Enzymes (inulinase, L-asparaginase and glucose oxidase) were successfully immobilized onto nanoparticles together with the biogenic synthesis of AgNPs and recyclability of the Inulinase-Pg-AgNPs complex was demonstrated. The study elaborates characteristics of green synthesized nanoparticles and their potential applications as anti-cancer, antibacterial and antioxidant nano drugs that could be used in food and nutraceutical industries. Enzyme immobilization on AgNPs without any toxic cross-linker opens up newer possibilites in enzyme-nanocomplex research.

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Section: Green Synthesis Of Agnpsmentioning

confidence: 97%

Characterization of biogenically synthesized silver nanoparticles for therapeutic applications and enzyme nanocomplex generation

2020

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“…The mean of ZOI against Staphylococcus aureus with antibiotics used in this study was high with ceftriaxone (28.29±0.43 mm) followed by tetracycline (24.65±0.29 mm) and less with cipro oxacin (8.15±0.28 mm) and ampicillin (4.29±0.27 mm). ZOI of ampicillin as 15.03 ± 0.54 mm against Staphylococcus aureus was reported (45) which was slightly near to the ZOI value of AgNO 3 solution. The study on AgNP's prepared by using Moringa oleifera, Tamarindus indica and comparing their antibacterial e cacy with antibiotics was less, but there is lot of scope for future study.…”

Section: Antibacterial Activity and Comparison Against Staphylococcus Aureusmentioning

confidence: 60%

Green Synthesis of Silver Nanoparticles Using Moringa Oleifera Leaf Extract, Tamarindus Indica Fruit Extract and Assessement of Antibacterial Activity Against Staphylococcus Aureus

Shirisha¹,

Kumar²,

R³

et al. 2021

Preprint

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The present study mainly deals with the green synthesis, characterization and evaluation of antibacterial properties of silver nanoparticles (AgNPs) synthesized by using the leaf extract of Moringa oleifera and fruit extract of Tamarindus indica. In this study for synthesis of silver nanoparticles different ratios of 1mM silver nitrate and Moringa oleifera leaf extract i.e, 95:5, 90:10 and 85: 15 was taken in conical flask and kept for one 1 hr at 25 0 c on magnetic stirrer, out of which 90:10 ratio was selected for further study based on highest peak, good size and stability. Tamarindus indica fruit extract was added to silver nitrate solution till the colour of the solution changes from light brown to chocolate brownish colour. The synthesized silver nanoparticles were characterized by UV-Visible spectroscopy, Zeta potential, size distribution by intensity. The absorption spectrum of the silver nano solution prepared by using Moringa oleifera and Tamarindus indica fruit extract showed a surface plasmon absorption band with maximum of 420 nm and 430 nm respectively indicating the presence of silver nanoparticles. The zeta value of silver nanoparticles synthesized from Moringa oleifera and Tamarindus indica fruit extract was -12.5 mV and -15.5 mV, size of 110.2 nm and 130.2 nm respectively. The antibacterial efficacy of nanosilver was checked by agar well diffusion method, and the silver nanoparticles showed effective antibacterial activity against Staphylococcus aureus.

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“…These nanoparticles proved to be well efficient in acting against the well known pathogens like S. aureus and E coli. Sonali Priyadarshini and team from Orissa Agricultural University (18,19) had conducted a study on Green Synthesis of Silver Nanoparticles using Azadirachta Indica and Ocimum Sanctum Leaf Extract wherein the authors have tested the antimicrobial activity of both Azadirachta and Ocimum against bacteria causing UTI infection like E coli, Klebsiella pneumonia and S aureus using Disc diffusion method. Silver Nanoparticles from Ocimum proved to be efficient with zone of 12.00± 0.65 mm for E coli, 9.66 ± 0.11 mm for K pneumonia and S aureus was inhibited to 23.00 ± 0.81 mm at 1mg/ml concentrations.…”

Section: Application Of Nano Technology In Water Purificationmentioning

confidence: 99%

A Review on Role of Nanoparticles and Active Compounds of Ocimum and Azadirahcta in Water Purification and Therapeutics

Hussein

2020

helix

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Water being the universal solvent and one of the first essential requirements of any life, demands an utmost care and attention for maintaining its quality and quantity on earth. Due to a high increase in the pollution rate and use of non bio-degradable substances, the water resources are contaminated day by day. It is very essential to purify the water not only from its physical impurities but also from its biological impurities and retain its nutritional quality. The current review focuses on the importance of various water purification techniques and the use of herbal extracts in the purification and quality enhancement of potable water. The review also highlights various herbs and Plants with medicinal values that can be used in water purification techniques.

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Green Synthesis of Silver Nanoparticles using <i>Azadirachta Indica</i> and <i>Ocimum Sanctum</i> Leaf Extract

Cited by 26 publications

References 0 publications

Characterization of biogenically synthesized silver nanoparticles for therapeutic applications and enzyme nanocomplex generation

Characterization of biogenically synthesized silver nanoparticles for therapeutic applications and enzyme nanocomplex generation

Green Synthesis of Silver Nanoparticles Using Moringa Oleifera Leaf Extract, Tamarindus Indica Fruit Extract and Assessement of Antibacterial Activity Against Staphylococcus Aureus

A Review on Role of Nanoparticles and Active Compounds of Ocimum and Azadirahcta in Water Purification and Therapeutics

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