Green synthesized nickel doped cobalt ferrite nanoparticles exhibit antibacterial activity and induce reactive oxygen species mediated apoptosis in MCF‐7 breast cancer cells through inhibition of PI3K/Akt/mTOR pathway

Abstract

This study aimed to synthesize the nickel (Ni) doped cobalt ferrite (CFO) nanoparticles and to determine its anticancer effect on breast cancer MCF‐7 cells. The various concentrations of Ni (0.2%, 0.4%, 0.6%, 0.8%, and 1%) doped CFO nanoparticles were synthesized using Coriandrum sativum extracts by precipitation technique. The synthesized Ni‐CFO was characterized by X‐ray diffraction, Fourier transform infrared spectroscopic, transmission electron microscopy, and vibrating sample magnetometer analyses. The results show that 0.8% Ni‐CFO nanoparticles showed good magnetic properties and antioxidant activity than other concentrations of Ni‐CFO. The results showed that the administration of 0.8% Ni‐CFO nanoparticles promoted apoptosis, and reduced cell adhesion and migration of MCF‐7 cells, as demonstrated by increased lipid peroxidation, decreased antioxidant levels such as superoxide dismutase, catalase, and glutathione peroxidase, and increased formation of reactive oxygen species. Moreover, administration of 0.8% Ni‐CFO nanoparticles decreased the Bcl‐2 expression while activating the expression of p53, Bax, and cleaved caspase 3, 8 and 9 protein expression. Notably, 0.8% Ni‐CFO treatment reduced phospho‐PI3K, phospho‐Akt, and phospho‐mTOR expression levels. As a result, via altering apoptotic related proteins, 0.8% Ni‐CFO induced cell death. Therefore, the 0.8% Ni‐CFO could be utilized to treat breast cancer.

Qibai Pingfei capsule induces apoptosis of pulmonary artery smooth muscle cells in hypoxic rats by regulating PI3K/AKT/mitochondrial signaling pathway

“…Many cytoprotective drugs or factors primarily activate or inhibit the PI3K/AKT signaling pathway. As a result, they affect the activity of Bcl-2 family proteins and exert antiapoptotic or pro-apoptotic effects [15][16][17]. The activated AKT (P-AKT) will be transferred to the cytoplasm or nucleus to further activate the activity of downstream proteins.…”

Green Synthesis of Cobalt Ferrite Nanoparticles: An Emerging Material for Environmental and Biomedical Applications

“…MTT cytotoxicity assay was performed on the U87 cell lines (in a human primary glioblastoma cell line with epithelial morphology) and no cytotoxicity effect was observed [60]. Additionally, Glycyrrhiza glabra (licorice) roots [61], C. microphylla fruit extract [37], tamarind extract [62,63], agar-agar from red seaweed (Rhodophyta) [64], Azadirachta indica leaves extract [65], Chenopodium album leaf extract [66], Coriandrum sativum extracts [67], verjuice extract [68], Moringa oleifera leaf extract [69], ginger root extract/Elettaria cardamomum seed extract [70], and baker's yeast (Saccharomyces cerevisiae) [71] were successfully used for green synthesis of cobalt ferrite and related compositions.…”