Green synthesized ZnO nanoparticles by Saccharomyces cerevisiae and their antibacterial activity and photocatalytic degradation

Abstract

Many research papers are currently being written about various methodologies for the green synthesis of ZnO nanoparticles. In this study, ZnO nanoparticles were prepared by green synthesis method using baker’s yeast (Saccharomyces cerevisiae). The characterization of the produced ZnO nanoparticles involved the utilization of XRD, FTIR, SEM, and TEM. The investigation involved the assessment of the antimicrobial potential and photocatalytic degradation efficacy of the synthesized materials through the utilization of Eriochrome Black T (EBT). The results indicate that the ZnO nanoparticles exhibit a spherical morphology, exhibiting diameters ranging from 13.0 to 20.0nm, with an average particle size of 15.0nm. The antimicrobial efficacy against (S. aureus) gram-positive and (E. coli) gram-negative bacteria was tested through the conduction of zone of inhibition (ZOI) and minimum inhibitory concentration (MIC) tests. This research focused on the study of the photocatalytic-degradation of EBT in the presence of ultraviolet light. The experiments focused on the impact of various factors, such as pH levels, initial EBT concentration, and nanocatalyst dosage, on the observed photocatalytic efficiency. In the in vitro ZOI and MIC results assay, it was observed that ZnO nanoparticles (NPs) exhibited greater efficacy against gram-positive S. aureus, with a ZOI measuring 23.1 mm and a MIC of 0.625 μg/ml. In contrast, the effectiveness of ZnO-NPs against gram-negative E. coli was comparatively lower, as indicated by a ZOI of 17.0 mm and a MIC of 1.250μg/ml. The greatest EBT elimination, which achieved 83.0% in equilibrium, was found employing 20.0mg of ZnO NPs at pH 3.0, according to the photocatalytic activity results. The synthetic ZnO NPs are powerful antibacterial agents that are effective against tested bacteria and may be used in the treatment of wastewater.

Novel highly efficient ternary ZnO wrapped PPy-NTs/g-C3N4 nanocomposite as an epoxy coating for corrosion protection

“…The O–H bending and stretching vibrational modes is also responsible for the peaks located at 1648 cm −1 52 . According to ZnO, the typical characteristic peaks can be detected at, 845, 1190, 1382, and 1456 cm −1 53 . The broad peak at about 3200 cm −1 is assigned to O–H stretching vibration of water 54 .…”

Immobilized lipase enzyme on green synthesized magnetic nanoparticles using Psidium guava leaves for dye degradation and antimicrobial activities

“…Employing plant extracts to synthesize nanoparticles also provides natural components for capping agents 3 . The primary objective of green synthesis research is to enhance a synthesis technique for creating environmentally friendly, non-toxic, superparamagnetic, and cost-effective magnetic nanoparticles 5 . The synthesis and characterization of magnetic nanoparticles, which are simply defined as particles with dimensions on the nanoscale that can react to magnetic fields, are increasingly attracting attention due to their exceptional capabilities in diverse applications.…”

“…The following section explains the current work approach of using zinc ferrite-magnetic nanoparticle particles to immobilize lipase enzymes. Magnetic nanocatalyst has the advantageous capability of being efficiently separated from the reaction medium through the use of an external magnet, hence obviating the necessity for additional filtration, centrifugation, or other laborious techniques 5 . Lipases, also known as triacylglycerol acyl hydrolases (EC 3.1.1.3), belong to the hydrolases family of enzymes specialized in catalyzing the hydrolysis of triacylglycerol (TAG) ester linkages, producing free fatty acids (FFAs) and glycerol.…”

Large-scale production of myco-fabricated ZnO/MnO nanocomposite using endophytic Colonstachys rosea with its antimicrobial efficacy against human pathogens

“…Another surprise is that ZnO and MnO NPs exhibit a variety of antimicrobial, antidiabetic, and acaricidal activities depending on their concentration, size, shape, and stability 23 – 25 . Many studies have previously bio-fabricated ZnO and/or MnO NPs using various microorganisms 7 , 26 , 27 . But very little studies have been done on myco-fabricating these metal oxides using endophytes.…”