Green Synthetized Selenium Nanoparticles Using Syzygium aromaticum (Clove) Extract Reduce Pentylenetetrazol-Induced Epilepsy and Associated Cortical Damage in Rats

Othman, Mohamed S.; Obeidat, Sofian; Aleid, Ghada Mohamed; Al-Bagawi, Amal H.; Fareid, Mohamed A.; Hameed, Reda S. Abdel; Mohamed, Kareem; Abdelfattah, Mohamed S.; Fehaid, Alaa; Hussein, Marwa M.; Aboelnaga, Shimaa Mohammed Hasnin; Moneim, Ahmed E. Abdel

doi:10.3390/app13021050

Cited by 10 publications

(8 citation statements)

References 60 publications

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“…Moreover, SeNPs prevented hippocampal cell loss, oxidative stress, and neuroinflammation, restored the balance between excitatory and inhibitory neuromediators, and in particular, normalized monoaminergic and cholinergic transmission, indicating that prodigiosin SeNPs can serve as a natural anticonvulsant due to its active antioxidant, anti-inflammatory, antiapoptotic, and neuromodulating properties [278]. Syzygium aromaticum-mediated SeNPs significantly restored proinflammatory cytokines (interleukin-1ß, interleukin-6 and tumor necrosis factor-α) to their normal levels and suppressed the activity of glial fibrillar acid protein, demonstrating their inhibitory effect on the inflammation associated with epilepsy [245]. Green-synthesized selenium nanoparticles using Spermacoce hispida as a carrier of s-allyl glutathione (a glutathione analogue) attenuated the APAP-toxicity-induced elevation of kidney and liver injury markers in the blood circulation.…”

Section: Discussionmentioning

confidence: 95%

“…In in vitro experiments, SeNPs biosynthesized by L. lactis capped with polysaccharides significantly attenuated the increase in IL-6, IL-8, IFN-γ, and TNF-α in IPEC-J2 cells [231]. The potential application of SeNPs using Syzygium aromaticum against epileptic seizures and damage to the cerebral cortex was shown in pentylenetetrazol-induced epilepsy in rats [245]. Green SeNPs reduced the levels of proinflammatory cytokines and suppressed the activity of the glial fibrillar acidic protein, showing their inhibitory effect on epilepsy-associated inflammation, and leading to their anti-inflammatory activity against PTZ-induced neuroinflammation.…”

Section: Anti-inflammatory Activitymentioning

confidence: 94%

“…Green SeNPs reduced the levels of proinflammatory cytokines and suppressed the activity of the glial fibrillar acidic protein, showing their inhibitory effect on epilepsy-associated inflammation, and leading to their anti-inflammatory activity against PTZ-induced neuroinflammation. The authors attribute this protective effect not only to the SeNP activity itself but also to the plant extract flavonoids, phenols, and GSH components as capping agents that can prevent free radical accumulation and reduce oxidative stress, subsequent inflammation, and apoptosis [245]. The anti-inflammatory effect of selenium nanoparticles may have the potential to reduce acute colitis symptoms.…”

Section: Anti-inflammatory Activitymentioning

confidence: 99%

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Selenium Nanoparticles: Green Synthesis and Biomedical Application

Mikhailova

2023

Molecules

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Selenium nanoparticles (SeNPs) are extremely popular objects in nanotechnology. “Green” synthesis has special advantages due to the growing necessity for environmentally friendly, non-toxic, and low-cost methods. This review considers the biosynthesis mechanism of bacteria, fungi, algae, and plants, including the role of various biological substances in the processes of reducing selenium compounds to SeNPs and their further packaging. Modern information and approaches to the possible biomedical use of selenium nanoparticles are presented: antimicrobial, antiviral, anticancer, antioxidant, anti-inflammatory, and other properties, as well as the mechanisms of these processes, that have important potential therapeutic value.

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Section: Discussionmentioning

confidence: 95%

Section: Anti-inflammatory Activitymentioning

confidence: 94%

Section: Anti-inflammatory Activitymentioning

confidence: 99%

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Selenium Nanoparticles: Green Synthesis and Biomedical Application

Mikhailova

2023

Molecules

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“…It activates astrocytes to generate glutamate, which leads to increased neuroexcitability linked with seizures [ 56 ]. The findings demonstrated that the PTZ injection resulted in a neuroinflammatory reaction, as indicated by a substantial rise in the protein concentration of cytokines ( IL-6, TNF-α, NF-κB, and IL-1β ) in the cerebral cortex [ 57 ]. Furthermore, the injection of PTZ resulted in a considerable rise in GFAP , which suggests the activation of astrocytes and subsequent inflammatory and apoptotic reactions in the brain [ 58 ].…”

Section: Discussionmentioning

confidence: 99%

Barbaloin Protects Pentylenetetrazol-Induced Cognitive Deficits in Rodents via Modulation of Neurotransmitters and Inhibition of Oxidative-Free-Radicals-Led Inflammation

Altyar,

Afzal,

Ghaboura

et al. 2024

Pharmaceuticals

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Background: Epilepsy is defined by an excessive level of activity in the neurons and coordinated bursts of electrical activity, resulting in the occurrence of seizure episodes. The precise cause of epileptogenesis remains uncertain; nevertheless, the etiology of epilepsy may involve neuroinflammation, oxidative stress, and malfunction of the neurotransmitter system. Objective: The goal of this investigation was to assess barbaloin’s protective properties with respect to pentylenetetrazol (PTZ)-)-induced cognitive deficits in rats via antioxidative, anti-inflammatory, and neurotransmitter-modulating effects. Methods: Wistar rats were subjected to PTZ [40 mg/kg (i.p.)], which induced cognitive decline. Behavior assessment using a kindling score, open-field test (OFT), novel object recognition test (NORT), and assays for superoxide dismutase (SOD), reduced glutathione (GSH), catalase (CAT), malondialdehyde (MDA), acetylcholinesterase (AChE), caspase-3, nitric oxide (NO), interleukins-1β (IL-1β), tumor necrosis factor-α (TNF-α), IL-6, nuclear factor kappa-B (NF-κB), Bcl-2 and Bax, and neurotransmitter levels [GABA, DA, NE, and serotonin (5-HT)] were performed. Results: The treatment of rats with barbaloin resulted in behavior improvement and significant changes in the levels of GSH, SOD, CAT, MDA, AChE, NO, IL-6, IL-1β, TNF-α, NF-κB, caspase-3, Bcl-2, and Bax compared to the PTZ control group. Barbaloin treatment resulted in notable changes in neurotransmitter levels (GABA, NE, 5-HT, DA) compared to the PTZ group. Conclusions: The ongoing study has gathered evidence indicating that the injection of barbaloin has resulted in significant improvements in cognitive performance in rats. This is achieved by inhibiting oxidative stress, enhancing the activity of natural antioxidant enzymes, reducing cytokine levels, and increasing the levels of neurotransmitters in the brain. These results were detected in comparison to a PTZ control and can be attributed to the potent anti-inflammatory and antioxidant capabilities of barbaloin, which could be linked to its neuroprotective properties. Barbaloin may potentially increase cognitive decline and boost neuronal survival by altering the expression of Bax, caspase-3, Bcl-2.

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“…Additionally, samples were treated with primary antibodies for detection of glial fibrillary acidic protein (GFAP) in astrocytes and caspase-3 [58]. For quantitative analysis, two independent observers manually counted the numbers of positive cells in five randomly chosen fields for each antibody using the image processing tool Image J.…”

Section: Estimation Of Pro-inflammatory Markersmentioning

confidence: 99%

Brain targeted lactoferrin coated lipid nanocapsules for the combined effects of apocynin and lavender essential oil in PTZ induced seizures

Youssef,

Boraie,

Ismail

et al. 2024

Drug Deliv. and Transl. Res.

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Apocynin (APO) is a plant derived antioxidant exerting specific NADPH oxidase inhibitory action substantiating its neuroprotective effects in various CNS disorders, including epilepsy. Due to rapid elimination and poor bioavailability, treatment with APO is challenging. Correspondingly, novel APO-loaded lipid nanocapsules (APO-LNC) were formulated and coated with lactoferrin (LF-APO-LNC) to improve br ain targetability and prolong residence time. Lavender oil (LAV) was incorporated into LNC as a bioactive ingredient to act synergistically with APO in alleviating pentylenetetrazol (PTZ)-induced seizures. The optimized LF-APO-LAV/LNC showed a particle size 59.7 ± 4.5 nm with narrow distribution and 6.07 ± 1.6mV zeta potential) with high entrapment efficiency 92 ± 2.4% and sustained release (35% in 72 h). Following subcutaneous administration, LF-APO-LAV/LNC brought about ⁓twofold increase in plasma AUC and MRT compared to APO. A Log BB value of 0.2 ± 0.14 at 90 min reflects increased brain accumulation. In a PTZ-induced seizures rat model, LF-APO-LAV/LNC showed a Modified Racine score of 0.67 ± 0.47 with a significant increase in seizures latency and decrease in duration. Moreover, oxidant/antioxidant capacity and inflammatory markers levels in brain tissue were significantly improved. Histopathological and immunohistochemical assessment of brain tissue sections further supported these findings. The results suggest APO/LAV combination in LF-coated LNC as a promising approach to counteract seizures. Graphical Abstract

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Green Synthetized Selenium Nanoparticles Using Syzygium aromaticum (Clove) Extract Reduce Pentylenetetrazol-Induced Epilepsy and Associated Cortical Damage in Rats

Cited by 10 publications

References 60 publications

Selenium Nanoparticles: Green Synthesis and Biomedical Application

Selenium Nanoparticles: Green Synthesis and Biomedical Application

Barbaloin Protects Pentylenetetrazol-Induced Cognitive Deficits in Rodents via Modulation of Neurotransmitters and Inhibition of Oxidative-Free-Radicals-Led Inflammation

Brain targeted lactoferrin coated lipid nanocapsules for the combined effects of apocynin and lavender essential oil in PTZ induced seizures

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