Green tea and vitamin C ameliorate some neuro-functional and biochemical signs of arsenic toxicity in rats

Sárközi, Kitti; Papp, András; Horváth, Edina; Máté, Zsuzsanna; Ferencz, Ágnes; Hermesz, Edit; Krisch, Judit; Paulik, Edit; Szabó, Andrea

doi:10.1179/1476830514y.0000000151

Cited by 23 publications

(13 citation statements)

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“…Changes in antioxidant parameters and peroxidation of membrane lipids under arsenic exposure have been documented in animal models [37,38]. Moreover, the ROS generated by arsenic are known to destabilize cell membranes via lipid peroxidation, which is a basic cellular deteriorating process in the liver [2].…”

Section: Discussionmentioning

confidence: 99%

Biochanin A Ameliorates Arsenic-Induced Hepato- and Hematotoxicity in Rats

Jalaludeen

Lee

et al. 2016

Molecules

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Biochanin A (BCA) is a natural organic compound of the phytoestrogenic isoflavone class that has antioxidant and metal chelator properties in the presence of transition metal ions, however, its efficacy in animal models is still obscure. Therefore, the objective of this study was to investigate the protective effects of BCA against arsenic-induced hepatic injury and hematotoxicity in rats. The results suggest that arsenic intoxicated rats showed significantly higher levels of plasma hepatic markers than normal control rats. Furthermore, an increase in lipid peroxidation with depletion of reduced glutathione (GSH) and activities of superoxide dismutase (SOD) and catalase (CAT) occurred in the livers of rats exposed to arsenic. Administration of BCA (20 mg/kg¨bw/day) and selenium (3 mg/kg¨bw/day) resulted in a significant reversal of hepatic and oxidative stress markers in arsenic-intoxicated rats. A low dose of BCA (10 mg/kg¨bw/day) did not show any preventive effect, while a high dose of BCA (40 mg/kg¨bw/day) partially prevented all hepatotoxicity events. These biochemical perturbations were supported by histopathological observations of the liver. Our results suggest that administration of BCA (20 mg/kg¨bw/day) attenuated the arsenic hepatotoxicity, a property that could contribute to the therapeutic approaches for chronic liver diseases.

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Section: Discussionmentioning

confidence: 99%

Biochanin A Ameliorates Arsenic-Induced Hepato- and Hematotoxicity in Rats

Jalaludeen

Lee

et al. 2016

Molecules

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“…Ivanov et al 11 demonstrated that AsA from green tea extracts could inhibit atherogenesis. In rats, AsA derived from green tea could help protect against the toxic effects of orally ingested arsenic and improve cellular antioxidative effects 12 , 13 .…”

Section: Introductionmentioning

confidence: 99%

Differentially expressed protein and gene analysis revealed the effects of temperature on changes in ascorbic acid metabolism in harvested tea leaves

Liu

et al. 2018

Hortic Res

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Tea is an important non-alcoholic beverage worldwide. Tea quality is determined by numerous secondary metabolites in harvested tea leaves, including tea polyphenols, theanine, caffeine, and ascorbic acid (AsA). AsA metabolism in harvested tea leaves is affected by storage and transportation temperature. However, the molecular mechanisms underlying AsA metabolism in harvested tea leaves exposed to different storage and transportation temperature conditions remain unclear. Here we performed RP-HPLC to detect dynamic changes in AsA content in tea leaves subjected to high- (38 °C), low- (4 °C), or room-temperature (25 °C) treatments. The AsA distribution and levels in the treated tea leaves were analyzed using cytological–anatomical characterization methods. The differentially expressed CsAPX1 and CsDHAR2 proteins, which are involved in the AsA recycling pathway, were identified from the corresponding proteomic data using iTRAQ. We also analyzed the expression profiles of 18 genes involved in AsA metabolism, including CsAPX1 and CsDHAR2. AsA was mainly distributed in tea leaf mesophyll cells. High- and low-temperature treatments upregulated the CsAPX1 and CsDHAR2 proteins and induced CsAPX and CsDHAR2 gene expression. These results indicated that the CsAPX1 and CsDHAR2 proteins might have critical roles in AsA recycling in tea leaves. Our results provide a foundation for the in-depth investigation of AsA metabolism in tea leaves during storage and transportation, and they will promote better tea flavor in tea production.

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“…The presence of epigenetic heterogeneity of developmentally important genes has important implications for assisted reproduction outcomes [34]. Another significant role was observed in the epigenetic regulation of stress reactivity in humans [35] and the formation and maintenance of memory in plasticity genes [36]. …”

Section: Discussionmentioning

confidence: 99%

Heterogeneous pattern of DNA methylation in developmentally important genes correlates with its chromatin conformation

2017

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BackgroundDNA methylation is a major epigenetic modification, playing a crucial role in the development and differentiation of higher organisms. DNA methylation is also known to regulate transcription by gene repression. Various developmental genes such as c-mos, HoxB5, Sox11, and Sry show tissue-specific gene expression that was shown to be regulated by promoter DNA methylation. The aim of the present study is to investigate the establishment of chromatin marks (active or repressive) in relation to heterogeneous methylation in the promoter regions of these developmentally important genes.ResultsChromatin-immunoprecipitation (ChIP) assays were performed to immuno-precipitate chromatin by antibodies against both active (H3K4me3) and repressive (H3K9me3) chromatin regions. The analysis of ChIP results showed that both the percentage input and fold enrichment of activated chromatin was higher in tissues expressing the respective genes as compared to the tissues not expressing the same set of genes. This was true for all the genes selected for the study (c-mos, HoxB5, Sox11, and Sry). These findings illustrate that inconsistent DNA methylation patterns (sporadic, mosaic and heterogeneous) may also influence gene regulation, thereby resulting in the modulation of chromatin conformation.ConclusionsThese findings illustrate that various patterns of DNA methylation (asynchronous, mosaic and heterogeneous) correlates with chromatin modification, resulting in the gene regulation.

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Green tea and vitamin C ameliorate some neuro-functional and biochemical signs of arsenic toxicity in rats

Abstract: Natural antioxidants might be useful in the protection of the central nervous system against the toxicity of oral As.

Cited by 23 publications

References 42 publications

Biochanin A Ameliorates Arsenic-Induced Hepato- and Hematotoxicity in Rats

Biochanin A Ameliorates Arsenic-Induced Hepato- and Hematotoxicity in Rats

Differentially expressed protein and gene analysis revealed the effects of temperature on changes in ascorbic acid metabolism in harvested tea leaves

Heterogeneous pattern of DNA methylation in developmentally important genes correlates with its chromatin conformation

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