Green Tea Catechins as Novel Antitumor and Antiangiogenic Compounds

Demeule, Michel; Michaud-Levesque, Jonathan; Annabi, Borhane; Gingras, Denis; Boivin, Dominique; Jodoin, Julie; Lamy, Sylvie; Bertrand, Y.; Béliveau, Richard

doi:10.2174/1568011023353930

Cited by 122 publications

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“…C, carbonate extraction was performed as described under "Experimental Procedures"; lysates were fractionated in a discontinuous sucrose density gradient. anti-angiogenic and anti-MMP inhibitory activities (35)(36)(37). We demonstrated that HA cell surface binding in control glioma cells treated for 1 h with either ilomastat or EGCg had no effect (Fig.…”

Section: Cholesterol Depletion Increases the Cell Surface Binding Of mentioning

confidence: 92%

Hyaluronan Cell Surface Binding Is Induced by Type I Collagen and Regulated by Caveolae in Glioma Cells

Annabi

Thibeault

Moumdjian

et al. 2004

Journal of Biological Chemistry

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Hyaluronan (HA) is a component of the brain extracellular matrix environment that is synthesized and secreted by glioma cells. The primary cell surface receptor for HA is CD44, a membrane glycoprotein that is functionally regulated by a membrane type 1 matrix metalloproteinase (MT1-MMP). Both CD44 and MT1-MMP are partially located in Triton X-100-insoluble domains, but no functional link has yet been established between them. In the present study, we studied the regulation of HA cell surface binding in U-87 glioma cells. We show that an MMP-dependent mechanism regulates the intrinsic cell surface binding of HA as ilomastat, a broad MMP inhibitor, increased HA binding to glioma cells. HA binding was also rapidly and specifically up-regulated by 3-fold by type I collagen in U-87 cells, which also induced a significant morphological reorganization associated with the activation of a latent form of MMP-2 through a MT1-MMP-mediated mechanism. Interestingly, caveolae depletion with a cell surface cholesteroldepleting agent ␤-cyclodextrin triggered an additional increase (9-fold) in the binding of HA, in synergy with type I collagen. On the other hand, HA cell surface binding was diminished by the MEK inhibitor PD98059 and by the overexpression of a recombinant, wild type MT1-MMP, whereas its cytoplasmic-deleted form had no effect. Taken together, our results suggest that MT1-MMP regulates, through its cytoplasmic domain, the cell surface functions of CD44 in a collagen-rich pericellular environment. Additionally, we describe a new molecular mechanism regulating the invasive potential of glioma cells involving a MT1-MMP/CD44/caveolin interaction, which could represent a potential target for anti-cancer therapies.The principal molecules that have been identified in the normal brain extracellular matrix (ECM) 1 are hyaluronan (HA; also known as hyaluronic acid, hyaluronate) and chondroitin sulfate (1, 2). HA is an important glycosaminoglycan constituent believed to be implicated in angiogenesis, the formation of new blood vessels from preexisting vasculature. Although the serum level of HA is already used as an indicator of progressive malignant disease (3), its effects on in vivo angiogenesis and endothelial cell (EC) function are complex and have been reported to depend on HA concentration and molecular size (4, 5). Accordingly, whereas high molecular weight HA was shown to inhibit EC functions (6), low molecular weight HA stimulated EC proliferation, tubulogenesis (6, 7), and neovascularization (8). Moreover, small HA polymers efficiently regulated CD44 cell surface functional expression and promoted tumor cell migration (9). Astrocytic tumors of the central nervous system express CD44 among other cell adhesion receptors of the integrin or immunoglobulin superfamily. Although HA is the principal ligand of CD44, other CD44 ligands include the ECM components collagen, fibronectin, laminin, and chondroitin sulfate, whereas mucosal addressin, serglycin, osteopontin, and the class II invariant chain represent ECM-unrelat...

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Section: Cholesterol Depletion Increases the Cell Surface Binding Of mentioning

confidence: 92%

Hyaluronan Cell Surface Binding Is Induced by Type I Collagen and Regulated by Caveolae in Glioma Cells

Annabi

Thibeault

Moumdjian

et al. 2004

Journal of Biological Chemistry

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“…2 The medicinal properties of tea leaves first appeared in a Chinese book on pharmaceutical plants (ca 200 BC). Later, in the Kissa Yojoki (Book of Tea, ca 1191), tea was listed as a remedy to control bleeding, help wounds heal, regulate body temperature, control blood sugar, and promote digestion.…”

Section: Discovery Of the Medicinal Properties Of Teasmentioning

confidence: 99%

“…Later, in the Kissa Yojoki (Book of Tea, ca 1191), tea was listed as a remedy to control bleeding, help wounds heal, regulate body temperature, control blood sugar, and promote digestion. 2,3 Green tea, which constitutes 20% of the tea manufactured worldwide, is probably the most consumed beverage besides water in Asian society ( Figure 1). The main substances found in green tea are caffeine (2-4%), amino acids (4%), lignin (6.5%), organic acids (1.5%), protein (15%), chlorophyll (0.5%), and the polyphenols (25-35%).…”

Section: Discovery Of the Medicinal Properties Of Teasmentioning

confidence: 99%

Green tea catechins for treatment of external genital warts

Meltzer

Monk

Tewari

2009

American Journal of Obstetrics and Gynecology

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This review evaluates the antiviral, antioxidant, and immunostimulatory properties of green tea catechins. Two randomized trials evaluating the activity and efficacy of green tea catechins in the management of external genital warts are presented, and the reported side effects associated with this topical treatment modality are outlined. Finally, the mechanism of action, percent of wart clearance, time to clearance, and toxicity profile of green tea catechins are compared with those of podofilox and imiquimod, 2 other patient-administered topical agents approved for treatment of anogenital warts.

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“…Some authors believe that differences between the effects of green tea observed in humans and in animal models are due to the use of higher catechin concentrations in the latter 17 .…”

Section: Resultsmentioning

confidence: 99%

Growth inhibition of Walker carcinosarcoma 256 with alcoholic extract of green tea leaves (Camellia sinensis)

et al. 2012

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PuRPOSE: To evaluate the antitumor activity of alcoholic extracts of green tea (Camella sinensis). METhOdS: Four groups of six Wistar rats were inoculated intramuscularly with 10 6 Walker tumor cells/mL. During 10 days, the animals received by gavage either 0.9% saline solution (Group I; negative control), solution containing 20 mg/Kg of tamoxifen (Group II; positive control), solution containing 0.07 g/Kg alcoholic extract of C. sinensis (Group III), or solution containing 0.14 g/Kg alcoholic extract of C. sinensis (Group IV). Following euthanasia on the tenth day, the tumor, liver, kidneys and spleen were excised and weighed, and tumor volume and tumor growth inhibition were quantified. RESuLTS: The average weight of the animals was greater in Group IV than in Group II (p=0.0107). Tumor weight was smaller in Group IV than in Group I (p=0.0062), but did not differ from Group II. Tumor volume was smaller in Groups II and IV than in Group I (p=0.0131). Tumor growth inhibition was observed in Groups II (44.67% ± 32.47), III (16.83% ± 53.02) and IV (66.4% ± 25.82) (p>0.05). The groups did not differ with regard to the weight of the excised organs. CONCLuSION: Alcoholic extracts of green tea have antitumor activity. Key words: Camellia sinensis. Catechin. Carcinoma 256, Walker. Rats. RESuMO ObJETIVO: Avaliar a atividade antitumoral do extrato alcoólico do chá verde (C. sinensis). MéTOdOS: Quatro grupos de seis ratos Wistar foram inoculados com 1x106 células/mL do tumor de Walker por via intramuscular. Os grupos foram tratados durante 10 dias, por gavagem, com salina 0,9 % (Grupo I, controle negativo), 20 mg/Kg de tamoxifeno (Grupo II, controle positivo) e extrato alcoólico de C. sinensis nas doses de 0,07 g/Kg (Grupo III) ou 0,14 g/Kg (Grupo IV). O volume e a inibição do crescimento tumoral foram calculados. RESuLTAdOS: A média dos pesos dos animais foi maior no Grupo IV do que no Grupo II (p=0,0107). O peso tumoral do Grupo IV foi menor do que o Grupo I (p=0,0062), mas não houve diferença quando comparado ao Grupo II. O volume tumoral foi menor nos grupos II e IV quando comparados ao Grupo I (p=0,0131). Inibição tumoral foi observada nos Grupos II = 44,67 ± 32,47, III = 16,83 ± 53,02 e IV = 66,4 ± 25,82 (p>0,05). Não houve diferença no peso dos órgãos entre os grupos. CONCLuSãO: O extrato alcoólico do chá verde possui ação antitumoral. descritores: Camellia sinensis. Catequina. Carcinoma 256 de Walker. Ratos. Growth inhibition of Walker carcinosarcoma 256 with alcoholic extract of green tea leaves (Camellia sinensis)Acta Cirúrgica Brasileira -Vol. 27 (9) 2012 -635

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Green Tea Catechins as Novel Antitumor and Antiangiogenic Compounds

Cited by 122 publications

References 0 publications

Hyaluronan Cell Surface Binding Is Induced by Type I Collagen and Regulated by Caveolae in Glioma Cells

Hyaluronan Cell Surface Binding Is Induced by Type I Collagen and Regulated by Caveolae in Glioma Cells

Green tea catechins for treatment of external genital warts

Growth inhibition of Walker carcinosarcoma 256 with alcoholic extract of green tea leaves (Camellia sinensis)

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