Green tea catechins inhibit Porphyromonas gulae LPS-induced inflammatory responses in human gingival epithelial cells

Yoshida, Sho; Inaba, Hiroaki; Nomura, Ryota; Nakano, Kazuhiko; Matsumoto‐Nakano, Michiyo

doi:10.1016/j.job.2022.05.006

Cited by 3 publications

(2 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Notably, LPS specifically mediates TLR4-activation and initiates downstream pathways, thereby releasing a series of inflammatory factors. In addition, LPS may activate TLR2 receptors and induce higher protein levels, causing an inflammatory response and oxidative stress. − Our previous study confirmed the presence of TLR2 and TLR4 as important receptors for PSPP-1. The results of the present study indicate that LPS treatment markedly increases both TLR2 and TLR4 expressions, whereas PSPP-1 treatment reduces their expressions.…”

Section: Discussionsupporting

confidence: 58%

Purple Sweet Potato Polysaccharide Exerting an Anti-inflammatory Effect via a TLR-Mediated Pathway by Regulating Polarization and Inhibiting the Inflammasome Activation

Song,

Niu,

Zhang

et al. 2024

J. Agric. Food Chem.

View full text Add to dashboard Cite

Purple sweet potato polysaccharide (PSPP-1) is a novel glucan; this study aimed to examine the anti-inflammatory effect of PSPP-1 and elucidate its potential mechanisms. Lipopolysaccharide (LPS)-induced RAW264.7 was used as the model of inflammation, cell viability, and levels of nitric oxide (NO), reactive oxygen species (ROS), and calcium ion (Ca 2+ ) were analyzed. ELISA and qPCR were used to assess the productions and mRNA expression of cytokines, and Western blotting was used to assess protein expressions in the TLR-mediated pathway, macrophage polarization, and inflammasome activation. The results demonstrated PSPP-1 inhibited cell proliferation and markedly decreased NO, ROS, and Ca 2+ levels. Moreover, PSPP-1 suppressed the secretions and mRNA expressions of pro-inflammatory cytokines and increased those of anti-inflammatory cytokines. Furthermore, PSPP-1 could exert anti-inflammatory effects through different pathways mediated by both TLR2 and TLR4, which modulated the expressions of essential proteins in the myeloid differentiation factor 88 (MyD88)-dependent and toll/IL-1 receptor domain-containing adaptor-inducing interferon-β (TRIF)-dependent signaling pathways. PSPP-1 even regulated the polarization of M1/M2 macrophages and inhibited the nucleotide oligomerization domain-like receptor protein 3 (NLRP3) inflammasome activation. These findings indicate that PSPP-1 can suppress LPS-induced inflammation via multiple pathways and may be a potential agent for therapeutic inflammation-related pathophysiological processes and disorders.

show abstract

Section: Discussionsupporting

confidence: 58%

Purple Sweet Potato Polysaccharide Exerting an Anti-inflammatory Effect via a TLR-Mediated Pathway by Regulating Polarization and Inhibiting the Inflammasome Activation

Song,

Niu,

Zhang

et al. 2024

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…Maruyama et al found that toothpaste containing green tea catechin alleviated gingival oxidative stress and periodontal inflammation in rats (Maruyama et al, 2011). At the cellular level, GTE significantly alleviated the inflammation in human gingival epithelial keratinocytes by decreasing the levels of interleukin-1β (IL-1β), IL-6, and tumor necrosis factor-α (TNF-α) (Hagiu et al, 2020;Yoshida et al, 2022). Besides, gargling with green tea or mouthwash containing GTE has been shown to prevent dental caries by increasing the pH of saliva, inhibiting acid formation, and reducing the activity of the glucosyltransferase enzyme produced by S. mutans (Sakti & Lawindra, 2023).…”

Section: Introductionmentioning

confidence: 99%

Green tea extracts alleviate acetic acid‐induced oral inflammation and reconstruct oral microbial balance in mice

Pan,

Lv,

Zhang

et al. 2023

Journal of Food Science

View full text Add to dashboard Cite

Oral cavity contains the second largest microbial community in the human body. Due to the highly vascularized feature of mouth, oral microbes could directly access the bloodstream and affect the host healthy systemically. The imbalance of oral microbiota is closely related to various oral and systemic diseases. Green tea extracts (GTE) mainly contain tea polyphenols, alkaloids, amino acid, flavones, and so on, which equipped with excellent anti‐inflammatory activities. Previous studies have demonstrated the beneficial effects of GTE on oral health. However, most researches used in vitro models or focused on limited microorganisms. In this study, the regulatory effect of GTE on oral microbiome and the alleviative effect on oral inflammation in vivo were evaluated. The results showed that GTE could efficiently alleviate the inflammations of the tongue, cheek pouch, as well as throat. GTE effectively inhibited the activation of NF‐κB through the upregulation of the anti‐inflammatory cytokine interleukin (IL)‐10, consequently leading to reduced expression of pro‐inflammatory cytokines IL‐6 and tumor necrosis factor‐α. The indexes of spleen and thymus were also elevated by GTE in stomatitis mice. Moreover, GTE promoted the growth of probiotics Lactobacillus and Bacillus, inhibited the reproduction of pathogens Achromobacter, reversing the microbiota disorders in oral cavity. This study not only presents a novel approach for enhancing oral microecology but also facilitates the wider adoption of tea consumption.

show abstract

Oral treatment with catechin isolated from Japanese green tea significantly inhibits the growth of periodontal pathogen Porphyromonas gulae and ameliorates the gingivitis and halitosis caused by periodontal disease in cats and dogs

Ohira,

Kaneki,

Shirao

et al. 2025

International Immunopharmacology

View full text Add to dashboard Cite

Green tea catechins inhibit Porphyromonas gulae LPS-induced inflammatory responses in human gingival epithelial cells

Cited by 3 publications

References 48 publications

Purple Sweet Potato Polysaccharide Exerting an Anti-inflammatory Effect via a TLR-Mediated Pathway by Regulating Polarization and Inhibiting the Inflammasome Activation

Purple Sweet Potato Polysaccharide Exerting an Anti-inflammatory Effect via a TLR-Mediated Pathway by Regulating Polarization and Inhibiting the Inflammasome Activation

Green tea extracts alleviate acetic acid‐induced oral inflammation and reconstruct oral microbial balance in mice

Oral treatment with catechin isolated from Japanese green tea significantly inhibits the growth of periodontal pathogen Porphyromonas gulae and ameliorates the gingivitis and halitosis caused by periodontal disease in cats and dogs

Contact Info

Product

Resources

About