Green Tea (–)-Epigallocatechin-3-Gallate Reduces Body Weight with Regulation of Multiple Genes Expression in Adipose Tissue of Diet-Induced Obese Mice

Abstract

Aims: The aim of this study was to investigate the antiobesity effect of (–)-epigallocatechin-3-gallate (EGCG) in diet-induced obese mice. Methods: Male C57BL/6J mice were fed on a high-fat diet for 8 weeks to induce obesity. Subsequently they were divided into 3 groups and were maintained on a high-fat control diet or high-fat diets supplemented with 0.2 or 0.5% EGCG (w/w) for a further 8 weeks. Changes in the expression of genes related to lipid metabolism and fatty acid oxidation were analyzed in white adipose tissue, together with biometric and blood parameters. Results: Experimental diets supplemented with EGCG resulted in reduction of body weight and mass of various adipose tissues in a dose-dependent manner. EGCG diet also considerably lowered the levels of plasma triglyceride and liver lipid. In the epididymal white adipose tissue of EGCG diet-fed mice, the mRNA levels of adipogenic genes such as peroxisome proliferator-activated receptor-γ (PPAR-γ), CCAAT enhancer-binding protein-α (C/EBP-α), regulatory element-binding protein-1c (SREBP-1c), adipocyte fatty acid-binding protein (aP2), lipoprotein lipase (LPL) and fatty acid synthase (FAS) were significantly decreased. However, the mRNA levels of carnitine palmitoyl transferase-1 (CPT-1) and uncoupling protein 2 (UCP2), as well as lipolytic genes such as hormone sensitive lipase (HSL) and adipose triglyceride lipase (ATGL), were significantly increased. Conclusion: These results suggest that green tea EGCG effectively reduces adipose tissue mass and ameliorates plasma lipid profiles in high-fat diet-induced obese mice. These effects might be at least partially mediated via regulation of the expression of multiple genes involved in adipogenesis, lipolysis, β-oxidation and thermogenesis in white adipose tissue.

Dietary <i>Rhus coriaria</i> L. powder reduces the blood cholesterol, VLDL-c and glucose, but increases abdominal fat in broilers

“…Nowadays, medicinal plants and their extracts or essential oils are the feed additives that are widely used in animal nutrition to improve fatty acid composition as well as the cholesterol contents of meat products. Dietary addition of additives such as turmeric (Daneshyar et al, 2012), oats (Lopez-Bote et al, 1998), garlic (Tapsell et al, 2006), thyme (Abdulkarimi et al, 2011), green tea (Wolfram et al, 2006Lee et al, 2009) and alfalfa (Ponte et al, 2004) have been tested in animals. Sumac (Rhus coriaria L.) is a plant species in the anacardiaceae family that is used as a spice and a native medicine.…”

Acute effects of epigallocatechin gallate from green tea on oxidation and tissue incorporation of dietary lipids in mice fed a high-fat diet

“…[5][6][7] This has been ascribed to the thermogenic action of green tea possibly due to a synergism of caffeine and catechins, which results in an extension of sympathetic stimulation of thermogenesis and thus increased energy expenditure. 6,8,9 However, recent evidence suggests that the major green tea catechin epigallocatechin gallate (EGCG) alone is able to decrease body fat accumulation [10][11][12][13] and development of fatty liver in mice fed a high-fat diet. 12,13 Apparently, the body fat reduction is mainly caused by decreased energy absorption and increased lipid oxidation as shown in animal models 10,14 and humans 15,16 although it cannot be excluded that an increase in energy expenditure too small to be measurable might also contribute.…”

Lemon Juice Counteracts the Effect of Green Tea Decoction on Body Weight Gains, High Fat Diet Induced-Liver Steatosis, Total Antioxidant Status and Some Metabolic Parameters in Rats

“…Hence, 15-min GTD prepared under realistic Tunisian habits could constitute a good method to preserve the beneficial properties of tea-polyphenols. The beneficial effect of 15-min GTD on body weight gains, LS and related metabolic parameter could be ascribe to the modulation of lipids and glucose digestibility and absorption as well as the regulation of genes expression involved in adipogenesis, lipolysis, beta-oxidation and thermogenesis in adipose tissue [7,[17][18][19]. Nevertheless, the decrease of iron storage in concomitance with the reduction of body gains, liver steatosis and the improvement of the antioxidant status is very important.…”