Green Tea Polyphenols as an Anti-Oxidant and Anti-Inflammatory Agent for Cardiovascular Protection

Tipoe, George L.; Leung, Tung-Ming; Hung, Ming-Wai; Fung, Ming Chiu

doi:10.2174/187152907780830905

Cited by 268 publications

(162 citation statements)

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“…Green tea has attracted much attention for its health benefits, epigallocatechin-3-gallate (EGCG) is the most active component of green tea and mediates most biological effects of green tea, including anti-tumor effect [3], anti-inflammatory [4], cardiovascular protection effect [5], hepatic fibrosis suppressive effect, and antioxidant activity [6].…”

Section: Introductionmentioning

confidence: 99%

EGCG attenuates pro-inflammatory cytokines and chemokines production in LPS-stimulated L02 hepatocyte

Liu

Qian

Chen

et al. 2014

ABBS

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Endotoxin lipopolysaccharide (LPS) plays an important role in the acceleration of inflammatory reaction of hepatitis as the second attack. Compounds that can prevent inflammation by targeting LPS have potential therapeutic clinical application. Epigallocatechin-3-gallate (EGCG) has potent hepatocyte-protective effect and mild anti-hepatitis virus function. Here, we investigated whether EGCG attenuated the severity of inflammatory response in LPS-stimulated L02 hepatocytes. L02 hepatocytes were pretreated with EGCG for 2 h, then stimulated by LPS at 250 ng/ml. The expression levels of chemokine regulated upon activation normal T-cell expressed and secreted (Rantes) and monocyte chemotactic protein-1 (MCP-1), pro-inflammatory cytokines tumor necrosis factor-a (TNF-a) and interferon-g, adhesion molecule intercellular adhesion molecule-1 (ICAM-1), oxidant stress molecules nitric oxide (NO), vascular endothelial growth factor (VEGF), and matrix metalloproteinase-2 (MMP-2) were tested by enzyme-linked immunosorbent assay. The expression of total extracellular signal-regulated kinase 1/2 (ERK1/2), phospho-ERK1/2 (p-ERK1/2), p-AKT, total p38, phospho-p38 (p-p38), total p65 and phospho-p65 (p-p65), IkBa, phospho-IkBa (p-IkBa) and TNF receptor associated factor 2 were tested by western blot analysis. Our results showed that pre-treatment with EGCG could significantly reduce the production of TNF-a, Rantes, MCP-1, ICAM-1, NO, VEGF, and MMP-2 in LPS-stimulated L02 hepatocytes in a dose-dependent manner. The effect of EGCG may be related to the inhibition of nuclear factor-kB (NF-kB) and mitogen-activated protein kinase (MAPK) signaling pathways by down-regulation of p-IkBa, p65, p-p65, p-p38, p-ERK1/2, and p-AKT. These results indicate that EGCG suppresses LPS-induced inflammatory response and oxidant stress and exerts its hepatocyte-protective activity partially by inhibiting NF-kB and MAPK pathways.

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Section: Introductionmentioning

confidence: 99%

EGCG attenuates pro-inflammatory cytokines and chemokines production in LPS-stimulated L02 hepatocyte

Liu

Qian

Chen

et al. 2014

ABBS

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“…1a), the most abundant polyphenolic extract from green tea, has been under extensive investigation after emerging evidence suggested that it may improve glucose metabolism, lipid metabolism and endothelial function and reduce the effects of arthritis, neurodegenerative diseases, hypertension, coronary heart disease, obesity, inflammation, cancer and insulin resistance. [15][16][17][18][19][20][21][22][23][24] Furthermore, this multifunctional polyphenol drastically reduces cognitive impairment and amyloid deposition in Alzheimer's transgenic mice. [25][26][27] Biochemical studies indicate that the neuroprotective action of EGCG results from its ability to inhibit protein aggregation, suppress/ enhance amyloidogenic/non-amyloidogenic proteolytic processes, scavenge free radicals, chelate iron and co-modulate various other cellular pathways.…”

Section: Introductionmentioning

confidence: 99%

(−)-Epigallocatechin-3-Gallate (EGCG) Maintains κ-Casein in Its Pre-Fibrillar State without Redirecting Its Aggregation Pathway

Hudson

Ecroyd

Dehle

et al. 2009

Journal of Molecular Biology

133

120

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The polyphenol (-)-epigallocatechin-3-gallate (EGCG) has recently attracted much research interest in the field of protein-misfolding diseases because of its potent anti-amyloid activity against amyloid-beta, alphasynuclein and huntingtin, the amyloid-fibril-forming proteins involved in Alzheimer's, Parkinson's and Huntington's diseases, respectively. EGCG redirects the aggregation of these polypeptides to a disordered offfolding pathway that results in the formation of non-toxic amorphous aggregates. whether this anti-fibril activity is specific to these disease-related target proteins or ismore generic remains to be established. In addition, the mechanism by which EGCG exerts its effects, as with all anti-amyloidogenic polyphenols, remains unclear. To address these aspects, we have investigated the ability of EGCG to inhibit amyloidogenesis of the generic model fibril-forming protein RCMkappa-CN (reduced and carboxymethylated kappa-casein) and thereby protect pheochromocytoma-12 cells from RCMkappa-CN amyloid-induced toxicity. We found that EGCG potently inhibits in vitro fibril formation byRCMkappa-CN [the IC50 for 50 uM RCMkappa-CN is 1 uM]. Biophysical studies reveal that EGCG prevents RCMkappa-CN fibril formation by stabilising RCMkappa-CN in its nativelike state rather than by redirecting its aggregation to the disordered, amorphous aggregation pathway. Thus, while it appears that EGCG is a generic inhibitor of amyloid-fibril formation, the mechanism by which it achieves this inhibition is specific to the target fibril-forming polypeptide. It is proposed that EGCG is directed to the amyloidogenic sheet-turn-sheet motif of monomeric RCMkappa-CN with high affinity by strong non-specific hydrophobic associations. Additional non-covalent pi-pi stacking interactions between the polyphenolic and aromatic residues common to the amyloidogenic sequence are also implicated. The polyphenol (−)-epigallocatechin-3-gallate (EGCG) has recently attracted much research interest in the field of protein-misfolding diseases because of its potent anti-amyloid activity against amyloid-β, α-synuclein and huntingtin, the amyloid-fibril-forming proteins involved in Alzheimer's, Parkinson's and Huntington's diseases, respectively. EGCG redirects the aggregation of these polypeptides to a disordered off-folding pathway that results in the formation of non-toxic amorphous aggregates. Whether this anti-fibril activity is specific to these disease-related target proteins or is more generic remains to be established. In addition, the mechanism by which EGCG exerts its effects, as with all anti-amyloidogenic polyphenols, remains unclear. To address these aspects, we have investigated the ability of EGCG to inhibit amyloidogenesis of the generic model fibril-forming protein RCMκ-CN (reduced and carboxymethylated κ-casein) and thereby protect pheochromocytoma-12 cells from RCMκ-CN amyloid-induced toxicity. We found that EGCG potently inhibits in vitro fibril formation by RCMκ-CN [the IC 50 for 50 μM RCMκ-CN is 13 ± 1 μM]. Biophys...

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“…All drinks possess different ingredients which have more or less strong antioxidant activities and additional beneficial effects. For example, green tea as one ingredient of the eQ-line contains antioxidants in high amounts (Cabrera et al, 2006;Speisky et al, 2006;Tipoe et al, 2007). Antioxidant activities were also reported for fruits and berries present in the drinks (for example, see Wilska-Jeszka, 1996;Anag-nostopoulou et al, 2004;Beattie et al, 2005;Hanamura et al, 2005;Netzel et al, 2005;Dai et al, 2007;Jayaprakashaa et al, 2007).…”

Section: Antioxidant and Antiinflammatory Potentialmentioning

confidence: 97%

Antioxidative and antiinflammatory potential of different functional drink concepts in vitro

Dartsch¹,

Kler²,

Kriesl³

2008

Phytotherapy Research

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The present study was undertaken to investigate the antioxidative effects of three different functional drink concepts especially designed to improve the body's performance and function and to possess high antioxidant activities. The concepts based on the mixture of various plant ingredients were: (1) eQ - equalize your nutrient balance, brain line [acerola-dragon fruit], (2) eQ - equalize your nutrient balance, beauty line [honey-pepper] and (3) Let's get red [intense]. By using a cell-based test assay, the study investigated the potential of the functional drinks to inactivate reactive superoxide anion radicals generated by inflammation-mediating cells as well as the effect on basal metabolism of these cells (antioxidant and antiinflammatory potential). In addition, by using a cell-free test assay the potential of the drinks to inactivate free exogenous superoxide anion radicals (scavenger effect) was investigated. The data presented here demonstrate the different radical scavenging, antioxidant and anti-inflammatory potential of the functional drink concepts. In particular Let's get red [intense] turned out to be the most potent drink in this respect and demonstrated marked efficacy in scavenging, antioxidant and antiinflammatory action.

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Green Tea Polyphenols as an Anti-Oxidant and Anti-Inflammatory Agent for Cardiovascular Protection

Cited by 268 publications

References 0 publications

EGCG attenuates pro-inflammatory cytokines and chemokines production in LPS-stimulated L02 hepatocyte

EGCG attenuates pro-inflammatory cytokines and chemokines production in LPS-stimulated L02 hepatocyte

(−)-Epigallocatechin-3-Gallate (EGCG) Maintains κ-Casein in Its Pre-Fibrillar State without Redirecting Its Aggregation Pathway

Antioxidative and antiinflammatory potential of different functional drink concepts in vitro

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