Green tea polyphenols change the profile of inflammatory cytokine release from lymphocytes of obese and lean rats and protect against oxidative damage

Molina, Nathalia; Bolin, Anaysa Paola; Otton, Rosemari

doi:10.1016/j.intimp.2015.08.011

Cited by 33 publications

(31 citation statements)

References 87 publications

(90 reference statements)

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“…We found that supplementation of diets with the two polyphenol‐rich plant products in overall had less or even no effect on the expression of genes involved in inflammation, cytoprotection, xenobiotic metabolism and unfolded protein response in the liver of piglets. This finding, which was confirmed by determination of selected proteins involved in these pathways, is in contradiction to several studies in rodents in which supplementation of polyphenols prevented inflammation and ER stress, improved the antioxidative and cytoprotective capacity by activation of Nrf2 and induced the hepatic xenobiotic metabolism (Panchal et al., ; Andrade et al., ; Molina et al., ; Pan et al., ; Xu et al., ). A likely explanation for these different results might be that the present study considered healthy piglets which were not subjected to any stressful treatment while the rodent studies cited in most cases used animals which were subjected to pro‐oxidative and proinflammatory treatments such as feeding a high‐fat diet.…”

Section: Discussionmentioning

confidence: 75%

“…Studies in the rodent model have shown that the liver, besides the small intestine, is another important target tissue of beneficial effects of polyphenols. For instance, it has been shown that polyphenols such as resveratrol or quercetin exert anti‐inflammatory effects and prevent the development of a fatty liver in rodents fed a high‐fat diet used as a model of non‐alcoholic steatosis (Panchal et al., ; Andrade et al., ; Molina et al., ; Xu et al., ; Kessoku et al., ). In these studies, anti‐inflammatory effects of polyphenols were induced by blocking the activation of nuclear factor‐kappa B (NF‐ κ B), by induction of nuclear factor E2‐related factor 2 (Nrf2) and by prevention of stress of the endoplasmic reticulum (ER stress).…”

Section: Introductionmentioning

confidence: 99%

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Effects of polyphenol‐rich plant products from grape or hop as feed supplements on the expression of inflammatory, antioxidative, cytoprotective and endoplasmic reticulum stress‐related genes and the antioxidative status in the liver of piglets

Geßner

Bonarius

Most

et al. 2016

Animal Physiology Nutrition

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Recent studies have shown that supplementation of plant products rich in polyphenols exerts anti-inflammatory effects in the small intestine and improves feed conversion in piglets. This study aimed to investigate whether dietary polyphenols have also anti-inflammatory and cytoprotective effects in the liver of piglets. For this end, relative mRNA concentrations of eight genes involved in proinflammatory pathways, eight genes involved in the antioxidative and cytoprotective system, six genes of phase I and phase II metabolism and 15 genes of the unfolded protein response (triggered by stress of the endoplasmic reticulum) in the liver of pigs fed diets supplemented with either 1% of grape seed and grape marc meal extract (GME) or 1% spent hops (SH) as sources of polyphenols were determined. Relative mRNA concentrations of almost all these genes, with few exceptions, in the liver of pigs supplemented with GME or SH did not differ from those in the liver of control piglets. Gene expression data were validated by consideration of concentrations of some selected proteins of these pathways which also did not differ between piglets supplemented with GME or SH and control piglets. Moreover, concentrations of thiobarbituric acid-reactive substances and tocopherols as well as the total antioxidant capacity in liver and plasma did not differ between pigs supplemented with either GME or SH and control piglets. Overall, this study shows that supplementation of GME or SH as sources of polyphenols does not influence hepatic pathways linked to inflammation, the antioxidant and cytoprotective system, stress of the endoplasmic reticulum and the xenobiotic system in healthy piglets.

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Section: Discussionmentioning

confidence: 75%

Section: Introductionmentioning

confidence: 99%

Effects of polyphenol‐rich plant products from grape or hop as feed supplements on the expression of inflammatory, antioxidative, cytoprotective and endoplasmic reticulum stress‐related genes and the antioxidative status in the liver of piglets

Geßner

Bonarius

Most

et al. 2016

Animal Physiology Nutrition

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“…Experimentally, green tea polyphenols have been shown to attenuate inflammatory functions of Ob rat neutrophils in terms of decreased release of ROS and proinflammatory cytokines [ 29 ]. Lastly, Molina et al [ 30 ] have documented in Ob rats, treated with green tea polyphenols, a reduced release of inflammatory cytokines and an increase in IL-10.…”

Section: Introductionmentioning

confidence: 99%

Immune Profile of Obese People and In Vitro Effects of Red Grape Polyphenols on Peripheral Blood Mononuclear Cells

Magrone

Jirillo

Spagnoletta

et al. 2017

Oxidative Medicine and Cellular Longevity

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The in vitro ability of polyphenols, extracted from red grape, to modulate peripheral blood mononuclear cell responses has been evaluated in 20 obese (Ob) people. With regard to cytokine release in response to phorbol myristate acetate (PMA), levels of interleukin-2 (IL-2), interferon-γ (IFN-γ), IL-4, IL-10, and IL-17 were higher in the Ob than in healthy (H) subjects. Vice versa, IL-21 concentrations were detected only in H people but they were undetectable in the Ob counterpart. In general terms, levels of IL-1β, IL-6, IL-8, and tumor necrosis factor-α were higher in Ob people when compared to H controls. On the other hand, polyphenols did not modify IFN-γ, IL-4, and IL-17 levels. However, an increase in IL-2 was observed in H individuals, whereas its levels were decreased in the Ob counterpart. Polyphenols significantly increased IL-10 release from H donors, whereas a trend to increase was observed in Ob people. In addition, polyphenols were able to significantly increase levels of H IL-21, while this was not the case in Ob people. Since IL-21 is an inducer of Th17 cells, it is likely that polyphenols may suppress the sources of this cytokine via production of IL-10. Accordingly, polyphenols decreased IL-1β and IL-6 release in comparison to H controls.

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“…Nosso grupo de pesquisa, assim como outros estudos, evidenciou uma redução nos níveis de citocinas pró-inflamatórias em ratos submetidos a uma dieta rica em gordura e carboidrato e suplementados com 500 mg/Kg de extrato de CV por 12 semanas. Após as 12 semanas de intervenção, observou-se uma redução das citocinas TNF-α, IL-1β e IL-6 por linfócitos e neutrófilos, possivelmente por sua ação imunomodulatória (Molina et al, 2015;Albuquerque et al, 2016). Além disso, nós também observamos que as catequinas do CV, sozinhas ou em combinação, são capazes de suprimir a ativação da sinalização TLR-4/NFκB p65 em neutrófilos humanos (Marinovic et al, 2015).…”

Section: Dentre Os Polifenóis Presentes Nounclassified

“…A EGCG é capaz de aumentar a fosforilação de tirosina no InsR e IRS-1 (insulin receptor substrate-1) através de mecanismos redox, como o aumento dos níveis de peróxido de hidrogênio (H2O2) (Waltner-Law et al, 2002). (Molina et al, 2015). Por outro lado, a EGCG também apresenta propriedades oxidativas.…”

Section: Dentre Os Polifenóis Presentes Nounclassified

Investigação do potencial termogênico e metabólico da planta <i>Camellia sinensis</i> em camundongos obesos - repercussão sistêmica do fenótipo bege.

Bolin¹

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Investigação do potencial termogênico e metabólico da planta Camellia sinensis em camundongos obesosrepercussão sistêmica do fenótipo bege São Paulo 2018 RESUMO BOLIN, A.P. Investigação do potencial termogênico e metabólico da planta Camellia sinensis em camundongos obesos -repercussão sistêmica do fenótipo bege. 2018. 105f. Tese (Doutorado em Farmacologia) -Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, 2018. Palavras-chave: Catequinas. Chá verde. Gasto Energético. Obesidade. Tecido adiposo. ABSTRACT BOLIN, A.P. Investigation of the thermogenic and metabolic potential of the Camellia sinensis plant in obese mice -systemic repercussion of the beige phenotype. 2018. 105p. Thesis (Ph. D. thesis in Pharmacology) -Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, 2018.The development of thermogenic/beige adipocytes in white adipose tissue (WAT) might reduce the adverse effects of obesity and could help to improve metabolic health. Certain pharmacological and nutritional agents can promote those effects by activating transcription factors or regulatory signaling pathways furthering WAT browning. As a nutritional or dietary supplement, compounds present in green tea (GT) has demonstrated remarkable correlations between energy metabolism and body weight (BW) loss, however, their potential contributions to the development of thermogenic/beige cells have been scarcely investigated. The aims of this study were to evaluate the relationship between metabolic alterations induced by an obesogenic diet and the induction of thermogenic/beige cells in GT-treated obese mice. We also evaluated the effect of a mix containing the main catechins found in GT (EGCG (2µM), EGC (1µM), ECG (0.6µM) and EC (0.4µM)) (Sigma) in a final concentration of 4µM and a GT extract at 0.19%.in the induction of beige phenotype in vitro (3T3-F442A preadipocytes). Here, we show that obese-induced mice (cafeteria diet/16 weeks) that were treated with GT (500 mg/kg of BW) for 12 weeks showed a significant reduction in indicators of obesity such as BW gain, fat depots, hyperlipidemia and an increment in plasma adiponectin levels without alteration in food intake. The basal metabolic rate was increased as well as genes involved in FA oxidation, thermogenesis and beige cells markers in the subcutaneous AT of obese animals treated with GT. GT also decreased inflammation and endoplasmic reticulum stress induced by the diet and relief insulin resistance (IR). We also find that our treatment was able to induce a futile cycle through de novo lipogenesis to activate the thermogenic pathway in vivo and in vitro. The mix of catechins or the GT showed significant reduction of lipid accumulation and upregulated the expression of thermogenic markers related to FA oxidation and mitochondrial activation, as well as, oxygen consumption. The effects of GT and the mix of catechins occur in an autonomous manner and seems to be dependent on initial mechanism in adipogenesis through PPARγ participation. Our study suggest that the me...

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Green tea polyphenols change the profile of inflammatory cytokine release from lymphocytes of obese and lean rats and protect against oxidative damage

Cited by 33 publications

References 87 publications

Effects of polyphenol‐rich plant products from grape or hop as feed supplements on the expression of inflammatory, antioxidative, cytoprotective and endoplasmic reticulum stress‐related genes and the antioxidative status in the liver of piglets

Effects of polyphenol‐rich plant products from grape or hop as feed supplements on the expression of inflammatory, antioxidative, cytoprotective and endoplasmic reticulum stress‐related genes and the antioxidative status in the liver of piglets

Immune Profile of Obese People and In Vitro Effects of Red Grape Polyphenols on Peripheral Blood Mononuclear Cells

Investigação do potencial termogênico e metabólico da planta <i>Camellia sinensis</i> em camundongos obesos - repercussão sistêmica do fenótipo bege.

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