Green Tea Polyphenols Reduce Body Weight in Rats by Modulating Obesity-Related Genes

Abstract

Beneficial effects of green tea polyphenols (GTP) against obesity have been reported, however, the mechanism of this protection is not clear. Therefore, the objective of this study was to identify GTP-targeted genes in obesity using the high-fat-diet-induced obese rat model. A total of three groups (n = 12/group) of Sprague Dawley (SD) female rats were tested, including the control group (rats fed with low-fat diet), the HF group (rats fed with high-fat diet), and the HF+GTP group (rats fed with high-fat diet and GTP in drinking water). The HF group increased body weight as compared to the control group. Supplementation of GTP in the drinking water in the HF+GTP group reduced body weight as compared to the HF group. RNA from liver samples was extracted for gene expression analysis. A total of eighty-four genes related to obesity were analyzed using PCR array. Compared to the rats in the control group, the rats in the HF group had the expression levels of 12 genes with significant changes, including 3 orexigenic genes (Agrp, Ghrl, and Nr3c1); 7 anorectic genes (Apoa4, Cntf, Ghr, IL-1β, Ins1, Lepr, and Sort); and 2 genes that relate to energy expenditure (Adcyap1r1 and Adrb1). Intriguingly, the HF+GTP group restored the expression levels of these genes in the high-fat-induced obese rats. The protein expression levels of IL-1β and IL-6 in the serum samples from the control, HF, and HF+GTP groups confirmed the results of gene expression. Furthermore, the protein expression levels of superoxide dismutase-1 (SOD1) and catechol-O-methyltransferase (COMT) also showed GTP-regulated protective changes in this obese rat model. Collectively, this study revealed the beneficial effects of GTP on body weight via regulating obesity-related genes, anti-inflammation, anti-oxidant capacity, and estrogen-related actions in high-fat-induced obese rats.

Caffeine-Stimulated Intestinal Epithelial Cells Suppress Lipid Accumulation in Adipocytes

“…Many studies have documented that adipocyte differentiation and lipid accumulation are associated with the development of obesity. Certain dietary compounds derived from plants are considered to be a prescription for anti-obesity by suppression of adipocyte differentiation and regulation of lipid metabolism in culture cells and animal experiments (22)(23)(24). However, there is little research that shows the effect of dietary compounds on adipogenesis through stimulation of intestinal epithelial cells.…”

Serum amyloid A is increased in children with abusive head trauma: a gel-based proteomic analysis

“…Serum concentrations of SAA, CC, HP, and PGDS in individual serum samples from cases and controls were analyzed by western blots as described previously (44). Briefly, 5 μl of serum sample was separated by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and then transferred to polyvinylidene fluoride membranes.…”

Hematological and serum biochemical parameters of blood in adolescent rats and histomorphological changes in the jejunal epithelium and liver after chronic exposure to cadmium and lead in the case of supplementation with green tea vs black, red or white tea

“…Polyphenols account for 25-35% of the dry matter of tea leaves (Chaturvedula and Prakash, 2011). Drinking tea prevents, among other conditions, obesity, diabetes, tumors, cardiovascular diseases, and damage to organs due to oxidative stress (Chung et al, 1998;Yang and Landau, 2000;Toschi et al, 2000;Priyadarshi et al, 2003;Wu et al, 2003;Davies et al, 2003;Arteel et al, 2005;van Dieren et al, 2009;Abolfathi et al, 2012;Lu et al, 2012;Fuhrman et al, 2013;Chen and Zhang, 2013;Mähler et al, 2013). Infusions and extracts of black, green and white tea were found to have strong antibacterial properties (Almajano et al, 2008;Flayyih et al, 2013;Radji et al, 2013).…”