“…While the peak of excretion is between 2 and 6 h after administration, it can be detected in urine through chromatographic techniques until 24-48 h after administration. Methylene blue-related greenish urine discoloration has been reported after several modes of administration of the dye: these include both direct intravenous infusion [11] and systemic absorption after oral administration [14,15], uterine filling during laparoscopic chromopertubation for infertility work-up [16], colon submucosal injections [17], irrigation of the bowel lumen [18], and manipulation of the paralytic ileum [19]. To date, only one case of urine discoloration after a methylene blue enema has been described in adults [20], and no similar reports exist in the pediatric literature.…”