Greenbug (Schizaphis graminum) herbivory significantly impacts protein and phosphorylation abundance in switchgrass (Panicum virgatum)

Zogli, Prince; Alvarez, Sophie; Naldrett, Michael J.; Palmer, Nathan A.; Koch, Kyle G.; Pingault, Lise; Bradshaw, Jeffrey D.; Twigg, Paul; Heng-Moss, Tiffany; Louis, Joe; Sarath, Gautam

doi:10.1038/s41598-020-71828-8

Cited by 12 publications

(18 citation statements)

References 87 publications

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“…The protein concentration was determined using the CB-X protein assay kit. One hundred μg of protein in 10 μl of the urea solution was diluted and digested with 1 μg trypsin (1:100 enzyme to substrate ratio) for 16 h overnight, and then an additional 1 μg of trypsin was added and digestion was continued for a further 3 h. Five hundred ng of each of the 9 secreted protein samples and 1 μg of each of the cytoplasmic protein samples was run by nano LC-MS/MS using a 2 h gradient on a 0.075 mm by 250 mm C 18 Waters CSH column feeding into a Q-Exactive HF mass spectrometer as described previously ( 49 ). All MS/MS samples were processed using Proteome Discoverer 2.2 software (Thermo) linked to the Mascot Server MS/MS search software (Matrix Science, London, UK, version 2.6.1).…”

Section: Methodsmentioning

confidence: 99%

“…The data were searched using a decoy database to set the false discovery rate to 1% (high confidence). The protein quantification was processed as described previously ( 49 ). The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE ( 50 ) partner repository with the data set identifier PXD023039.…”

Section: Methodsmentioning

confidence: 99%

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Staphylococcus aureus Responds to Physiologically Relevant Temperature Changes by Altering Its Global Transcript and Protein Profile

Bastock

Marino

Wiemels

et al. 2021

mSphere

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Staphylococcus aureus is an opportunistic pathogen that colonizes the anterior nares of 30 to 50% of the population. Colonization is most often asymptomatic; however, self-inoculation can give rise to potentially fatal infections of the deeper tissues and blood. Like all bacteria, S. aureus can sense and respond to environmental cues and modify gene expression to adapt to specific environmental conditions. The transition of S. aureus from the nares to the deeper tissues and blood is accompanied by changes in environmental conditions, such as nutrient availability, pH, and temperature. In this study, we perform transcriptomics and proteomics on S. aureus cultures growing at three physiologically relevant temperatures, 34°C (nares), 37°C (body), and 40°C (pyrexia), to determine if small scale, biologically meaningful alterations in temperature impact S. aureus gene expression. Results show that small but definite temperature changes elicit a large-scale restructuring of the S. aureus transcriptome and proteome in a manner that, most often, inversely correlates with increasing temperature. We also provide evidence that a large majority of these changes are modulated at the posttranscriptional level, possibly by sRNA regulatory elements. Phenotypic analyses were also performed to demonstrate that these changes have physiological relevance. Finally, we investigate the impact of temperature-dependent alterations in gene expression on S. aureus pathogenesis and demonstrate decreased intracellular invasion of S. aureus grown at 34°C. Collectively, our results demonstrate that small but biologically meaningful alterations in temperature influence S. aureus gene expression, a process that is likely a major contributor to the transition from a commensal to pathogen. IMPORTANCE Enteric bacterial pathogens, like Escherichia coli, are known to experience large temperature differences as they are transmitted through the fecal oral route. This change in temperature has been demonstrated to influence bacterial gene expression and facilitate infection. Staphylococcus aureus is a human-associated pathogen that can live as a commensal on the skin and nares or cause invasive infections of the deeper tissues and blood. Factors influencing S. aureus nasal colonization are not fully understood; however, individuals colonized with S. aureus are at increased risk of invasive infections through self-inoculation. The transition of S. aureus from the nose (colonization) to the body (infection) is accompanied by a modest but definite temperature increase, from 34°C to 37°C. In this study, we investigate whether these host-associated small temperature changes can influence S. aureus gene expression. Results show widespread changes in the bacterial transcriptome and proteome at three physiologically relevant temperatures (34°C, 37°C, and 40°C).

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Staphylococcus aureus Responds to Physiologically Relevant Temperature Changes by Altering Its Global Transcript and Protein Profile

Bastock

Marino

Wiemels

et al. 2021

mSphere

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“…Moreover, Septima with respect to Quintus at the control (70% SWC) as well as severe drought (40% SWC) revealed an enhanced relative abundance in spot 3803 identified as lipoxygenase-2 and ssp 3503 identified as aldehyde dehydrogenase 7b which are involved in the detoxification of lipid peroxides that arise as products of hydrogen peroxide reaction with unsaturated fatty acids. In the study of Zogli et al. (2020) , four lipoxygenases were identified in the response of switchgrass ( Panicum virgatum ) to greenbug infestation.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, more than 100 papers corresponding to “plant proteome and aphid” have been found in the Web of Science database (accessed in June 2022). Aphids induced the accumulation of several defense modulators such as terpene synthase, papain-like cysteine protease, serine carboxypeptidase, and lipoxygenase and the repression of photosynthesis-related proteins in plants ( Zogli et al., 2020 ). Nguyen et al.…”

Section: Introductionmentioning

confidence: 99%

Proteomic responses of two spring wheat cultivars to the combined water deficit and aphid (Metopolophium dirhodum) treatments

et al. 2022

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In the field, plants usually have to face the combined effects of abiotic and biotic stresses. In our study, two spring wheat cultivars—Septima and Quintus—were subjected to three water regimes [70%, 50%, and 40% soil water capacity (SWC)], aphid (Metopolophium dirhodum) infestation, or the combination of both stresses, i.e., water deficit (50%, 40% SWC) and aphids. The study has a 2 × 3 × 2 factorial design with three biological replicates. In the present study, the results of proteomic analysis using 2D-DIGE followed by MALDI-TOF/TOF protein identification are presented. Water deficit but also aphid infestation led to alterations in 113 protein spots including proteins assigned to a variety of biological processes ranging from signaling via energy metabolism, redox regulation, and stress and defense responses to secondary metabolism indicating a long-term adaptation to adverse conditions. The absence of specific proteins involved in plant response to herbivorous insects indicates a loss of resistance to aphids in modern wheat cultivars during the breeding process and is in accordance with the “plant vigor hypothesis.” Septima revealed enhanced tolerance with respect to Quintus as indicated by higher values of morphophysiological characteristics (fresh aboveground biomass, leaf length, osmotic potential per full water saturation) and relative abundance of proteins involved in mitochondrial respiration and ATP biosynthesis.

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“…Based on a number of studies (Bilgin et al, 2010;Singh et al, 2013), there is usually a decrease in the intensity of metabol-ic processes (including a decrease in the rate of protein synthesis) under conditions of biotic stress; it has a protective nature, thus keeping a vital potential of cells necessary for subsequent repair. In addition, it has been reported (Zogli et al, 2020) that Schizaphis graminum (Rondani, 1852) (Hemiptera) feeding on the leaves of Panicum virgatum L. inhibits the accumulation of proteins involved in photosynthesis. In our study, it was shown that accumulation of soluble protein content was observed in most plantings and, especially, in age group І.…”

Section: а вmentioning

confidence: 99%

Assessment of Parectopa robiniella Clemens (Lepidoptera: Gracillariidae) Effect on Biochemical Parameters of Robinia pseudoacacia Under Conditions of an Industrial City in Steppe Ukraine

Шупранова

Holoborodko

Лоза

et al. 2022

Ekológia (Bratislava)

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Antioxidant enzymatic activity and soluble protein content in Robinia pseudoacacia L. (black locust), invasive tree in Europe, plants were determined under feeding conditions of the caterpillars of Parectopa robiniella Clemens, 1863, invasive miner in Europe, in trees of three (I, II, III) age groups (5–10, 15–25, and 40 years). Biochemical analysis data showed significant differences between reference and experimental leaf samples in different urban ecosystems. There was no association between changes in catalase and peroxidase activity and protein content under the influence of leaf blotch miners depending on the tree age. Significant variability of proteins under P. robiniella influence was noted; the highest of its values were recorded in the leaves of ІІІ-aged trees. In most plantings (and especially in trees of age group III), an increase (1.2 to 2.7 times) in GPx activity or the absence of its changes was registered both in the ecologically favorable zone and in the locations affected by emissions from road transport and industrial enterprises. Plants of age groups I and II in the emission areas of metallurgical, pipe-rolling, and chemical industries experienced a significant decrease in GPx activity (by 20.2–44.6%) in the leaves damaged by the phytophage. In most of the plantings studied, catalase showed an increase/stability of its activity in all age groups of trees under condition of pest attack. The highest increase in САТ activity (2.1 times) was recorded in young R. pseudoacacia plants in an ecologically favorable area of the city.

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Greenbug (Schizaphis graminum) herbivory significantly impacts protein and phosphorylation abundance in switchgrass (Panicum virgatum)

Cited by 12 publications

References 87 publications

Staphylococcus aureus Responds to Physiologically Relevant Temperature Changes by Altering Its Global Transcript and Protein Profile

Staphylococcus aureus Responds to Physiologically Relevant Temperature Changes by Altering Its Global Transcript and Protein Profile

Proteomic responses of two spring wheat cultivars to the combined water deficit and aphid (Metopolophium dirhodum) treatments

Assessment of Parectopa robiniella Clemens (Lepidoptera: Gracillariidae) Effect on Biochemical Parameters of Robinia pseudoacacia Under Conditions of an Industrial City in Steppe Ukraine

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