Latvia is one of the leaders in production and use of forest biofuel in Europe. The rapid increase of forest biofuel market raises questions about sustainability of the supply chains and contribution of the forest biofuel produced in Latvia to the climate change mitigation. Sustainability of forest biofuel is addressed in multiple recent international political initiatives; particularly, the European 2030 climate and energy package and the nature restoration regulation. Climate change mitigation potential of forest biofuel is surrounded by multiple speculations, which have to be addressed by comprehensive evaluation of greenhouse gas (GHG) emissions due to production and delivery of forest biofuel. According to the study results, average GHG emissions due to delivery of harvesting residues from the state forests correspond to 1.4 kg CO2 eq GJ-1, including forwarding, comminution and delivery to a 68 km distance. This is significantly less than the default values provided in the regulation (EC) 2018/2001, particularly during the delivery of forest biofuel. GHG emissions due to delivery of forest biofuel from removal of vegetation in abandoned farmlands are 1.9 kg CO2 eq GJ-1, from forest drainage ditches – 1.7 kg CO2 eq GJ-1, from pre-commercial thinning – 2.1 kg CO2 eq GJ-1. Estimation of the GHG emissions is complicated by limited information on some of the sources and productivity. Building of the system for collection of activity data is a crucial task for transparent demonstration of GHG emissions and the effect of applied mitigation measures.