Greening pharmaceutical applications of liquid chromatography through using propylene carbonate–ethanol mixtures instead of acetonitrile as organic modifier in the mobile phases

Tache, Florentin; Udrescu, Stefan; Albu, Florin; Micăle, Florina; Medvedovici, Andrei

doi:10.1016/j.jpba.2012.11.045

Cited by 50 publications

(15 citation statements)

References 21 publications

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“…This indicated that the gradient eluent using acetonitrile was suitable for applications requiring low UV detection wavelengths. This inference is supported by previous studies, which have reported that though methanol and acetonitrile are commonly used for modifying reversed-phase chromatography, acetonitrile is more favorable as it performs at a lower UV cut-off than methanol [28,35]. Taken together, it can be inferred that shorter analyte retention can be achieved from acetonitrile interference.…”

Section: Optimization Of Chromatographic Conditionsupporting

confidence: 71%

Development of a Simple High-Performance Liquid Chromatography-Based Method to Quantify Synergistic Compounds and Their Composition in Dried Leaf Extracts of Piper Sarmentosum Roxb.

Purba

Paengkoum

2021

Separations

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There is a growing demand to enhance pharmaceutical and food safety using synergistic compounds from Piper sarmentosum Roxb., such as polyphenols and water-soluble vitamins. However, information on standardized analytical methods to identify and quantify these compounds of interest is limited. A reversed-phase high-performance liquid chromatography with diode-array detection (HPLC-DAD)-based method was developed to simultaneously detect and quantify the amounts of tannin, flavonoid, cinnamic acid, essential oil, and vitamins extracted from P. sarmentosum leaves using methanol, chloroform, and hexane. Commercially and non-commercially-cultivated P. sarmentosum leaves were subjected to seven different drying treatments (shade; sun; air oven at 40 °C, 60 °C, 80 °C, and 100 °C; and freeze-drying) for three consecutive months. Most compounds were detected most efficiently at a detection wavelength of 272 nm. The developed method displayed good detection limits (LOD, 0.026–0.789 µg/mL; LOQ, 0.078–2.392 µg/mL), linearity (R2 > 0.999), precision (%RSD, <1.00), and excellent accuracy (96–102%). All P. sarmentosum leaf extracts were simultaneously tested and analytically compared without time-consuming fractionation. Methanolic plant extracts showed better peak area and retention time splits compared to chloroformic and hexanoic extracts. Differences in synergistic compound composition were dependent on the type of drying treatment but not on cultivation site and time of sampling. Flavonoid was identified as the dominant phytochemical component in P. sarmentosum leaves, followed by the essential oil, cinnamic acid, ascorbic acid, and tannin. Overall, we present a simple and reproducible chromatographic method that can be applied to identify different plant compounds.

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Section: Optimization Of Chromatographic Conditionsupporting

confidence: 71%

Development of a Simple High-Performance Liquid Chromatography-Based Method to Quantify Synergistic Compounds and Their Composition in Dried Leaf Extracts of Piper Sarmentosum Roxb.

Purba

Paengkoum

2021

Separations

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“…In addition, PC is commercially available as an HPLC-grade solvent at reasonable prices. However, PC has two major drawbacks which obviously limit the possibility of a direct transposition from ACN to PC in RP-HPLC [ 59 , 60 ]. The first drawback refers to the low miscibility of PC with water, which is why a third solvent is often added, such as MeOH or EtOH.…”

Section: Alternative Organic Solvents In Rp-hplcmentioning

confidence: 99%

“…Some applications of PC in RP-HPLC have been reported for the determination of active substances in pharmaceutical formulations, but most of them used MeOH as a ternary solvent [ 57 , 58 , 61 , 62 , 63 ]. Nevertheless, Tache et al [ 59 ] studied the possibility of substituting ACN with PC and EtOH mixtures in RP-HPLC. Three series of compounds have been analyzed, including acidic (fenofibric, lovastatic, and simvastatic acids), neutral (toluene, fluorene, and fluoranthene), and basic (carbamazepine, diltiazem, and nicergoline) compounds.…”

Section: Alternative Organic Solvents In Rp-hplcmentioning

confidence: 99%

Greening Reversed-Phase Liquid Chromatography Methods Using Alternative Solvents for Pharmaceutical Analysis

et al. 2018

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The greening of analytical methods has gained increasing interest in the field of pharmaceutical analysis to reduce environmental impacts and improve the health safety of analysts. Reversed-phase high-performance liquid chromatography (RP-HPLC) is the most widely used analytical technique involved in pharmaceutical drug development and manufacturing, such as the quality control of bulk drugs and pharmaceutical formulations, as well as the analysis of drugs in biological samples. However, RP-HPLC methods commonly use large amounts of organic solvents and generate high quantities of waste to be disposed, leading to some issues in terms of ecological impact and operator safety. In this context, greening HPLC methods is becoming highly desirable. One strategy to reduce the impact of hazardous solvents is to replace classically used organic solvents (i.e., acetonitrile and methanol) with greener ones. So far, ethanol has been the most often used alternative organic solvent. Others strategies have followed, such as the use of totally aqueous mobile phases, micellar liquid chromatography, and ionic liquids. These approaches have been well developed, as they do not require equipment investments and are rather economical. This review describes and critically discusses the recent advances in greening RP-HPLC methods dedicated to pharmaceutical analysis based on the use of alternative solvents.

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“…solutions of formic acid with concentrations of 0.1-0.2% and acetonitrile. Other methods include substitution of acetonitrile with mixtures of propylene carbonate and ethanol (Tachea, Udrescua, Albua, Micălea, & Medvedovici, 2013). For a limited number of compounds (e.g.…”

Section: Introductionmentioning

confidence: 99%

New approach for determination of the degradation products of fenspiride hydrochloride found in oral liquid formulations

Cioroiu

Caba

Prisăcaru

et al. 2018

Biomedical Chromatography

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Fenspiride hydrochloride (FNS) is used in treating chronic inflammatory diseases, most commonly as a liquid oral solution. FNS produces degradation products along with fenspiride N-oxide (FNO) and 1-phenylethyl-4-hydroxy-4-aminomethyl piperidine hydrochloride (PHAP). We aimed to develop and validate a chromatographic method in order to identify the main degradation products in the presence of other compounds from a liquid preparation. The method used a dual gradient using two buffer solutions: the first with pH 4.5 (buffer 1, pH 4.5-MeOH 90:10%, v/v) and the second with pH 2.9 (buffer 2, pH 2.9-acetronitrile-methanol, 65:15:10%, v/v/v). As mentioned, there was a modification of the organic mixture, starting with 10% methanol and ending with a mixture of acetonitrile-methanol (15:10%, v/v). The flow-rate was 1.5 mL/min. According to the elution program, experimental conditions started with 100% solution S1, which decreased to 0% and, simultaneously, solution S2 increased to 100% during the first 10 min and was maintained for a further 5 min. After 15 min, initial conditions were re-established. The linearity interval was 0.5-2 μg/mL and the minimum correlation coefficient was 0.999. The recovery factor was 100.47-103.17% and the limit of quantification was 0.19-0.332 μg/mL. Intra-day maximum precision was 4.08% for FNS and 2.65% for PHAP. This double-gradient mobile phase produced good specificity in relation to the degradation products of FNS and other constituents of the oral liquid formulation. Forced degradation studies revealed other related substances that were confirmed in mass balance analyses. Degradation products were confirmed in acidic, basic and oxidative media.

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Greening pharmaceutical applications of liquid chromatography through using propylene carbonate–ethanol mixtures instead of acetonitrile as organic modifier in the mobile phases

Cited by 50 publications

References 21 publications

Development of a Simple High-Performance Liquid Chromatography-Based Method to Quantify Synergistic Compounds and Their Composition in Dried Leaf Extracts of Piper Sarmentosum Roxb.

Development of a Simple High-Performance Liquid Chromatography-Based Method to Quantify Synergistic Compounds and Their Composition in Dried Leaf Extracts of Piper Sarmentosum Roxb.

Greening Reversed-Phase Liquid Chromatography Methods Using Alternative Solvents for Pharmaceutical Analysis

New approach for determination of the degradation products of fenspiride hydrochloride found in oral liquid formulations

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