Grk7 but not Grk1 undergoes cAMP-dependent phosphorylation in zebrafish cone photoreceptors and mediates cone photoresponse recovery to elevated cAMP

Chrispell, Jared D.; Xiong, Yubin; Weiss, Ellen R.

doi:10.1016/j.jbc.2022.102636

Cited by 5 publications

(4 citation statements)

References 76 publications

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“…Grk1b and Grk7a both contributed to the restoration of the isolated cone photoresponse in larval ZF [28]. Mutations in GRK1A can disrupt normal rhodopsin regulation and impair the activity of the phototransduction pathway, and, thus, vision, in ZF [29]. The top upregulated gene following EDZ exposure, the heat shock cognate 70-kd protein, like (hsp70l), was reported to be involved in embryonic lens formation [30].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Profiling of Etridiazole-Exposed Zebrafish (Danio rerio) Embryos Reveals Pathways Associated with Cardiac and Ocular Toxicities

Vasamsetti,

Chon,

Yoon

et al. 2023

IJMS

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Etridiazole (EDZ) is a thiadiazole-containing fungicide commonly used to control Pythium and Phytophthora spp. Although previous studies have shown that EDZ is teratogenic, the exact molecular mechanisms underlying its toxicity remain unknown. In this study, a zebrafish (Danio rerio; ZF) model was used to explore the molecular pathways associated with EDZ toxicity. The whole transcriptome of ZF embryos exposed to 96 h of EDZ was analyzed, along with developmental abnormalities. EDZ-induced malformations were primarily related to the eyes, heart, and growth of the ZF. Compared to untreated ZF, etridiazole-treated ZF had 2882 differentially expressed genes (DEGs), consisting of 1651 downregulated genes and 1231 upregulated genes. Gene ontology enrichment analysis showed that DEGs were involved in biological processes, such as sensory perception, visual perception, sensory organ development, and visual system development, and showed transmembrane transporter and peptidase regulator activities. Metabolism, phototransduction, aminoacyl-tRNA biosynthesis, MAPK signaling pathway, calcium signaling pathway, and vascular smooth muscle contraction were among the most enriched KEGG pathways. The qPCR analyses of the eight random genes were in good agreement with the transcriptome data. These results suggest several putative mechanisms underlying EDZ-induced developmental deformities in ZF.

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Section: Discussionmentioning

confidence: 99%

Transcriptome Profiling of Etridiazole-Exposed Zebrafish (Danio rerio) Embryos Reveals Pathways Associated with Cardiac and Ocular Toxicities

Vasamsetti,

Chon,

Yoon

et al. 2023

IJMS

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“…PKA-dependent phosphorylation of GRK1 in mouse and GRK7 in Xenopus models was light-dependent [ 48 , 49 ], although cAMP-mediated phosphorylation of GRK1 affects the kinetics of dark adaptation in mouse rod but not cone photoreceptors [ 50 ]. Conversely, in zebrafish cones, cAMP-mediated phosphorylation downregulates GRK7 but not its cone-specific ortholog GRK1b [ 51 ]. Taken together, these data suggest that at least one key element of the phototransduction cascade, rhodopsin kinase, is downregulated in the dark via inhibition of its activity by PKA-mediated phosphorylation after Ca-mediated rise of cAMP.…”

Section: Targets Of Camp Regulationmentioning

confidence: 99%

“…Taken together, these data suggest that at least one key element of the phototransduction cascade, rhodopsin kinase, is downregulated in the dark via inhibition of its activity by PKA-mediated phosphorylation after Ca-mediated rise of cAMP. Thus, the mechanism of phosphorylation is specific for GRK1 in rods and GRK7 in cones and, therefore, may involve different downstream kinases with different specificities [ 51 ].…”

Section: Targets Of Camp Regulationmentioning

confidence: 99%

Multiple Roles of cAMP in Vertebrate Retina

Erofeeva

Meshalkina

Firsov

2023

Cells

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cAMP is a key regulatory molecule that controls many important processes in the retina, including phototransduction, cell development and death, growth of neural processes, intercellular contacts, retinomotor effects, and so forth. The total content of cAMP changes in the retina in a circadian manner following the natural light cycle, but it also shows local and even divergent changes in faster time scales in response to local and transient changes in the light environment. Changes in cAMP might also manifest or cause various pathological processes in virtually all cellular components of the retina. Here we review the current state of knowledge and understanding of the regulatory mechanisms by which cAMP influences the physiological processes that occur in various retinal cells.

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“…In the current study, human EYS was localized at the connecting cilia (CC) and OS in photoreceptor cells, whereas mutant truncated EYS did not distribute to these regions. We found that EYS plays a role in the trafficking of an OS protein, G protein-coupled receptor kinase 7 (GRK7), which is evolutionarily conserved along with EYS and involved in photoresponse recovery (27,28). EYS-RD organoids and zebrafish exhibited light-induced photoreceptor cell death related to GRK7 deprivation in the OS.…”

Section: Introductionmentioning

confidence: 99%

Phototoxicity avoidance is a potential therapeutic approach for retinal dystrophy caused by EYS dysfunction

Otsuka,

Imamura,

Oishi

et al. 2024

JCI Insight

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Grk7 but not Grk1 undergoes cAMP-dependent phosphorylation in zebrafish cone photoreceptors and mediates cone photoresponse recovery to elevated cAMP

Cited by 5 publications

References 76 publications

Transcriptome Profiling of Etridiazole-Exposed Zebrafish (Danio rerio) Embryos Reveals Pathways Associated with Cardiac and Ocular Toxicities

Transcriptome Profiling of Etridiazole-Exposed Zebrafish (Danio rerio) Embryos Reveals Pathways Associated with Cardiac and Ocular Toxicities

Multiple Roles of cAMP in Vertebrate Retina

Phototoxicity avoidance is a potential therapeutic approach for retinal dystrophy caused by EYS dysfunction

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