Road dust (RD) is one of the most important sources of particles in the atmosphere, especially in industrial areas and cities. In this special issue, we collected 16 original articles that describe field, experimental, and modeling studies related to RD and its various size fractions as a key issue in understanding the relationships between several urban and industrial environments and in the identification of pollution sources. Articles in the special issue focus primarily on the following main topics: (1) study of the chemical composition and speciation of RD and its source attribution; (2) assessment of RD and aerosol pollution levels (including express technique), environmental hazards and public health risks; (3) distribution of stable and radioactive isotopes in RD; (4) determination of factors affecting the level of dust accumulation on roads and the intensity of its pollution; and (5) study of the effect of RD on the atmosphere and other environments. Based on the results presented in this special issue, but not limited to, some of the current challenges in studying RD are formulated, including the need for further geographically wider and analytically deeper work on various aspects of the formation, transport pathways, and accumulation of RD in urban, industrial and other areas.