Gross and chromosomal cytology of virus infected Chinese hamster cells

Mazzone, H.M.; Yerganian, George

doi:10.1016/0014-4827(63)90336-7

Cited by 27 publications

(6 citation statements)

References 3 publications

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“…A low proportion were isochromatid breaks. Similar primary effects, with breakage of chromosomes in cell cultures derived from different animal species, have been found after infection with a number of different viruses, such as herpes simplex, SW 40, polyoma virus, adenovirus -12 (HAMPAR and ELLISON, 1963; MAZZONE and YERGANIAN, 1963;STICH et of., 1964;KOPROWSKI et al, 1962;YERGANIAN et al, 1962;SHEIN et ENDERS, 1963;YOGT and DULBECCO, 1963;COOPER and BLACK, 1963;PONTEN et ctl., 1963;WOLMAN et d., 1964; MOORHEAU and SAK-SELA, 1965). Further, a n extreme form of chromosome breakage with pronounced fragmentation, described as pulverization of chromosomes, has been observed by and by HARNDEN (1964).…”

supporting

confidence: 56%

Chromosome Studies in Some Virus Infections

Gripenberg

2009

Hereditas

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supporting

confidence: 56%

Chromosome Studies in Some Virus Infections

Gripenberg

2009

Hereditas

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“…It should also be emphasized that many viruses seem to be associated with this type of chromosome change including herpes (14,15), SV40 (16--18), polyoma (19) and Schmidt-Ruppin Rous (3) in vitro in Chinese hamster and human diploid and Syrian hamster and rat tissue culture respectively, and measles and chickenpox in vivo. We must first emphasize that there is no proof of firm correlation or association between chromosome breakage and mahgnancy in general.…”

Section: Have Been Studied Inmentioning

confidence: 99%

Measles associated chromosome breakage

Nichols¹,

Levan

1965

Archiv f Virusforschung

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In 1962 we reported chromosome breaks in peripheral white blood cells (1) of patients with clinical measles (rubeola). This work was part of a study on the relationship between viruses, chromosomes, and carcinogenesis. The first system studied in this project was a tumor virus, the Schmidt-Ruppin strain of the Rous sarcoma virus. This produces tumors not only in the chicken and fowl but also in a wide variety of mammals (2). When this virus was studied in the rat it was noted that chromosome breakage occurred when the tumor was studied in vivo or in tissue culture and also when the virus was added to normal rat cells in tissue culture (3). When the chromosomes of the rat tumors induced by this virus were studied in serial transplantation, new chromosome types were seen to arise. This could only occur as the result of previous breakage and reunion or healing between the broken ends of chromosomes.When rat tumors of "0" passage were put into tissue culture and serially transferred, they underwent a cellular transformation with change in morphology and growth characteristics. At the time these cellular changes took place, large numbers of chromosome breaks and rearrangements were seen. After this, the cells stabilized and behaved as a cell line. The breaks and rearrangements had not been seen in preparations made before the morphologic transformation and have not been seen since that time. Finally, when cell-free virus was added to diploid rat embryo cells in tissue culture and the first and second divisions after the addition were studied ehromosomally, it was found that there was an increase in the number of breaks over the controls. On the basis of these observations it was felt that breakage was playing a role in the interaction of the cell and tumor virus.

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“…hr(9), and were prominent at 4 hr. A 1-hr pulse of 3H-thymidine, applied just before the 4-hr harvest, generally yielded no evidence of DNA synthesis in affected metaphases.…”

mentioning

confidence: 92%