Gross and Histologic Evaluation of Abdominal Adhesions Associated with Chromic Catgut and Polypropylene Sutured Enteropexies in Dog

Akinrinmade, J. F.; Lawal, A O

doi:10.4067/s0717-95022010000400037

Cited by 4 publications

(3 citation statements)

References 10 publications

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“…The extensive adhesion response in animals submitted to hysterorrhaphy with catgut has been attributed to the inflammatory reaction to the suture wire type, which has been corroborated by similar observations in urinary bladder suturing of rats . Lymphocyte predominance in the catgut group, in comparison with the control group, may be explained by its action for up to 120 h in a normal inflammatory process; this justifies the presence of adhesions during the remodeling phase in inflammatory events with the formation of fibrinogen‐rich exudates …”

Section: Discussionsupporting

confidence: 57%

“…8 Lymphocyte predominance in the catgut group, in comparison with the control group, may be explained by its action for up to 120 h in a normal inflammatory process 9 ; this justifies the presence of adhesions during the remodeling phase in inflammatory events with the formation of fibrinogen-rich exudates. 10 Chitosan, poly [(1,4)-D-glucose-2-amine], (C 6 H 11 IN 4 ) n , is the deacetylated form of chitin (polysaccharide mainly found in crustaceans, insects, fungi, and algae exoskeleton). The use of chitosan in sutures is of interest because it has very low toxicity and complete biodegradation.…”

Section: Discussionmentioning

confidence: 99%

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Macroscopic, histochemical, and immunohistochemical comparison of hysterorrhaphy using catgut and chitosan suture wires

et al. 2015

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The objective of the current article was to compare the quality of the healing process after hysterorrhaphy with catgut and chitosan suture wires via macroscopic, histochemical, and immunohistochemical evaluations. Seven ewes were submitted to a video-assisted laparotomy. A hysterectomy was performed with subsequent catgut and chitosan hysterorrhaphy in both horns. Then, macroscopic evaluation and biopsies of the hysterorrhaphy and control areas were carried out after 30 days. Although postoperative adhesion was observed in five animals of the catgut group, no adhesions were detected in the chitosan one. Additionally, there was a significant difference in increasing collagen type III and connective tissue for the chitosan group when compared to the control and catgut groups. A larger amount of blood vessels was also observed in the control group in comparison with the catgut and chitosan groups. Collagen type I and vascular endothelial growth factor-A (VEGF-A) were significantly different among groups. Thus, chitosan promoted a preventive effect on postoperative adhesion formation in the hysterorrhaphy areas, with increasing collagen type III deposition during tissue mending, which granted an enhanced healing process.

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Section: Discussionsupporting

confidence: 57%

Section: Discussionmentioning

confidence: 99%

Macroscopic, histochemical, and immunohistochemical comparison of hysterorrhaphy using catgut and chitosan suture wires

et al. 2015

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“…Intestinal plication (or enteropexy), as is also defined experimentally produces significant morbidity, resulting in vomiting, diarrhea, anorexia, constipation, obstruction, adhesions, perforation and septic peritonitis, especially after a month of surgery [18]. Research with enteropexy execution in dogs observed a gradual increase of adhesions from seven to 30 days after surgery [1].…”

Section: Introductionmentioning

confidence: 99%

Morphometric Alteration of Intestinal Epithelium of Rats (Rattus norvegicus) Submitted to the Technique of Enteropexy

Queiróz

Silva

Filgueira

et al. 2016

Acta Scientiae. Vet.

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Background: Due to the numerous complications that enteropexy may cause in domestic animals and humans, this study aimed at investigating the microscopic level, the damage generated by this technique in mice, in order to investigate the morphometric changes caused by enteropexy technique in rats.Materials, Methods & Results: Eighteen Wistar rats were submitted to surgical technique of enteropexy. To evaluate intestinal disorders they were euthanized at 30 (Group I), 60 (group II) and 90 (group III) days after the procedure. The animals were perfused with 2.5% glutaraldehyde and 4% paraformaldehyde for the removal of intestinal fragments for analysis in conventional microscopy and scanning electron microscopy. In classical histopathology, group I presented mild lesions with necrosis of the epithelium and infiltration of mononuclear cells. After 60 days, extensive ischemic necrosis area was observed, characterized by the denudation of epithelium cells and cellular debris in the lumen surface and loss of intestinal crypts. After 90 days (group III) lesions were evident and were characterized by strong coagulative necrosis of epithelial cells and intestinal crypts. Under electron microscopy, group I showed early fusion of the adjacent intestinal walls. After 60 days there was adherence of luminal stenosis and intestinal walls. In group III loss of epithelium and substitution of necrotic tissue were identified.Discussion: In the current study it was noticed microscopically that the groups had inflammatory reactions to foreign bodies. It is known that the sutures, independent of manufacture, behave as foreign bodies that induce tissue inflammation of the recipient organism and could harm the scar repair. Among the surgical threads, the nylon type (which was chosen for the current experiment) is indicated in the approach tissues in general and bandages, including cardiovascular, ophthalmic and neurological procedures. This category wire induces minimal inflammation of the receptor tissue. In studies of the histopathological analysis of the tissue reactions produced by the wire implant or nylon thread clamp in rats, it was observed that when the local action of two implants were compared, inflammation showed qualitatively similar responses, although they had different characteristics regarding their course. Inflammation is proportional to the proliferation of fibroblasts and the presence of fibrous tissue around the suture. This quote confirms the analyzed samples, in which, in all postoperative times showed inflammatory process, although at different magnitudes. Surgical research in laboratory animals has expanded in recent decades, mainly due to better anesthetic support, the sophistication of infrastructure, material for perioperative continuous monitoring and an incessant search for species that reproduce human morbid conditions. The main focus of these studies have been improving the knowledge about the pathophysiological mechanisms of disease, undertake therapeutic trials with new drugs, studying biomarkers and evaluate new techniques to be applied prospects in man. The most common sites of occurrence of intestinal are ileocolic intussusception junction and jejunojejunal segment. The enteroplication has been used to reduce the occurrence of intussusception, promoting the adhesion of the adjacent serosa layers of intestinal segments and thereby decreasing intestinal motility, their applicability is reported in humans, dogs and cats. The enteropexy technique causes morphological changes in intestinal epithelium of rats, evidenced in conventional and electron microscopy, progress with the passing of time the procedure.

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Efficacy of gelatin sponge in the prevention of post-surgical intra-abdominal adhesion in a rat model

Ibrahim

Kamel

Soliman

2022

Research in Veterinary Science

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Gross and Histologic Evaluation of Abdominal Adhesions Associated with Chromic Catgut and Polypropylene Sutured Enteropexies in Dog

Cited by 4 publications

References 10 publications

Macroscopic, histochemical, and immunohistochemical comparison of hysterorrhaphy using catgut and chitosan suture wires

Macroscopic, histochemical, and immunohistochemical comparison of hysterorrhaphy using catgut and chitosan suture wires

Morphometric Alteration of Intestinal Epithelium of Rats (Rattus norvegicus) Submitted to the Technique of Enteropexy

Efficacy of gelatin sponge in the prevention of post-surgical intra-abdominal adhesion in a rat model

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