Records of spiders of the vineyard terraces from the vicinity of the villages Morkůvky and Mutěnice Jesličky (South Moravia, Czech Republic) are published in the presented work. Spiders were collected by pitfall traping during 30 April -28 October 2011 and by day and night sweeping of herb vegetation in diff erent dates of 2011. In total 2719 individuals were collected, and identifi ed as 139 species of 23 families. Eighteen species listed in the Red List of threatened species in the Czech Republic were recorded. In vineyard terraces in the vicinity of Morkůvky, 12 threatened species (CR -2 species, EN -3 species, VU -7 species) were found altogether. In the vineyard terraces at Mutěnice, we found 13 threatened spider species (CR -2 species, EN -6 species, VU -5 species). The most signifi cant fi ndings are Alopecosa solitaria (Herman, 1879), Cheiracanthium pennyi O.P.-Cambridge, 1873; Dipoena coracina (C. L. Koch, 1837); Dysdera hungarica Kulczyński, 1897; Eresus kollari Rossi, 1846; Euryopis quinqueguttata Thorell, 1875; Sibianor tantulus (Simon, 1868) and Haplodrassus minor (O.P.-Cambridge, 1879). Besides of these threatened species, we discovered rich spectra of rare spiders related to the well preserved xeric grasslands. Our faunistic results indicate that artifi cial habitats, such as vineyard terraces, are important refuges for wide spectra of xerothermophilous spiders.