The objectives of present investigation were to test the effects on water use efficiency (WUE) and cotton yield of implementing a range of deficit irrigation regimes triggered at specific fractions of root zone soil moisture, field capacity (θ fc) and different crop phenological stages. The study was conducted on southern oasis of the Taklamakan desert, China. The cotton crop's WUE was quantified, as were leaf photosynthesis and yield. From a photosynthetic perspective, deficit irrigation resulted in 16.8%, 10.3% and 2.2% increases in leaf WUE under θ fc-based regulated deficit irrigation (T1, T2, and T3), compared to the control, respectively. Cotton yield and its components were significantly affected by irrigation depths (p≤0.05). A relatively high seed yield (0.65 kg/m 3) and the highest WUE were achieved, under T3 (70% θ fc at seedling stage, 60% θ fc at squaring, 50% θ fc at full-bloom, 70% θ fc at boll, 70% θ fc at boll cracking stage), showing it to be the most effective and productive irrigation schedule tested. As the application of θ fc-based deficit irrigation in surface-irrigated cotton fields showed great potential in saving water, maintaining a high WUE, and improving cotton seed yield, a management strategy consisting or irrigation thresholds of 70% θ fc in the root zone at the seedling, boll and boll cracking stages, and of 60% θ fc at the squaring stage, and 50% θ fc at the full-bloom stage, would be recommended for this extremely arid region.