Water quality has degraded dramatically in Wen-Rui Tang River watershed, Zhejiang, China, especially due to rapid economic development since 1995. This paper aims to assess spatial and temporal variations of the main pollutants (NH + 4 -N, TN, BOD 5 , COD Mn , DO) of water quality in Wen-Rui Tang River watershed, using the geographic information system, cluster analysis (CA) and principal component analysis (PCA). Results showed that concentrations of BOD 5 , COD Mn , NH + 4 -N, and TN were significantly higher in tertiary rivers than in primary and secondary rivers. From April 2006 to March 2007, the concentrations of NH + 4 -N (2.25-57.9 mg/L) and TN (3.78-70.4 mg/L) in all samples exceeded Type V national water quality standards (≥2 mg/L), while 5.3% of all COD Mn (1.83-27.5 mg/L) and 4 -N and TN in tertiary rivers had a significant negative correlation with 5-day cumulative rainfall and monthly rainfall, while there were no significant correlations in primary and secondary rivers. The results of CA and spatial analysis showed that the northern part of WenRui Tang River watershed was the most seriously polluted. This region is characterized by the high population density and industrial and commercial activities. The PCA and spatial analysis indicated that the degraded water quality is caused by anthropogenic activities and poor wastewater management.