Groundwater may represent a significant pathway for nutrients and other dissolved solutes into Florida Bay, especially near the Keys where wastewater disposal practices add large amounts of nitrogen and phosphorus to the subsurface each year. Previously, we suggested that high water column inventories of the tracers 222 Rn and CH 4 may be indicative of groundwater discharge. In this study, we employed mass balance calculations to determine that the total benthic fluxes required to maintain the measured water column tracer inventories were significantly larger than diffusive fluxes and varied between 4.2-5.6 dpm m Ϫ2 min Ϫ1 and 5.8-15.4 nmoles m Ϫ2 min Ϫ1 for 222 Rn and CH 4 , respectively. Independent estimates of the diffusive flux and porewater activities/concentrations allowed us to calculate an advective groundwater velocity, assuming that the difference between the total benthic flux (given above) and the diffusive flux is driven by seepage-driven porewater advection. These calculated velocities ranged from 0.2 to 4.3 cm d Ϫ1 for all sites, tracers, and sampling periods, with a best estimate of approximately 1.7 cm d
Ϫ1. These estimates of groundwater velocities compare very well with previous measurements of groundwater flux (1-3 cm d Ϫ1 ) at the same sites via seepage meters.Florida Bay, a mosaic of shallow water banks and deeper water basins, was once characterized by clear waters and healthy seagrass meadows. Massive seagrass die-offs, planktonic algal blooms, and salinity excursions have occurred in the last decade (Boesch et al. 1993;Phlips et al. 1995;Phlips and Badylak 1996;Phlips et al. 1999;Fourqurean and Robblee 1999). There is no simple answer available for what has caused these dramatic events, but it is certain that they are related to a combination of natural phenomena and anthropogenic activities. A better understanding of the complete hydrologic and nutrient budget of Florida Bay is essential for interpreting past and current change, as well as for development of good management strategies for this and other coastal systems.Some patterns of ecological change and environmental degradation in Florida Bay point to increased nutrient loading as one likely cause (Lapointe et al. 1990). However, the sources and pathways of these hypothesized nutrient additions are not clear. Nutrient inputs to the bay include freshwater flowing from the Everglades, Gulf of Mexico waters flowing into the bay, atmospheric deposition, and local sources (Rudnick et al. 1999). Localized inputs include diffusion from sediments, nutrient release during sediment re-