“…Antibiotics are significant drugs for treating bacterial infections and are widely applied in medicine, animal husbandry, and agriculture. − Ciprofloxacin (CIP) and norfloxacin (NOR), the third-generation synthetic quinolone antibiotics, have been extensively prescribed for several different types of human infections due to their high potency, favorable bioavailability, the broad spectrum of activity, high serum concentrations, oral and intravenous formulations, and relatively low incidence of side effects. − Nevertheless, alarmingly increased use of CIP and NOR has led to high levels of antibiotic residues, which pose a serious threat to environmental safety and human health. , The overdose and incomplete metabolization of CIP and NOR make them easy to accumulate in the body, causing serious damage to humans, such as hepatotoxicity, hematuria, and cholestatic jaundice. − Numerous methods have been explored so far for the detection of antibiotics, such as spectrophotometry, liquid chromatography-mass spectrometry, capillary electrophoresis, and high-performance liquid chromatography. − However, the complicated operation, time-consuming nature, and high cost of these methods limit their practical application. − Owing to its advantages of high sensitivity, economic feasibility, and easy operation, fluorescence detection has received extensive attention in modern chemical analysis, which is considered a promising candidate for the sensitive detection of antibiotic residues. ,, Consequently, it is significant to develop excellent fluorescence probes with ultra-low detection limits to detect the presence of CIP and NOR in human urine.…”