Groundwater, soil and compost, as possible sources of virulent and antibiotic-resistant Pseudomonas aeruginosa

Kaszab, Edit; Radó, Júlia; Kriszt, Balázs; Pászti, Judit; Lesinszki, Virág; Szabó, Adám; Tóth, Gergő; Khaledi, Ariane; Szoboszlay, Sándor

doi:10.1080/09603123.2019.1691719

Cited by 27 publications

(23 citation statements)

References 64 publications

(68 reference statements)

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“…To this end, we screened three environmental sources where P. aeruginosa is commonly present: sewage, soil, and river [ 30 , 31 ]. We isolated 18 Pseudomonas phages from these environments in different cities, Rasht and Hamadan, in Iran.…”

Section: Introductionmentioning

confidence: 99%

Sewage and sewage-contaminated environments are the most prominent sources to isolate phages against Pseudomonas aeruginosa

Aghaee¹,

Mirzaei²,

Alikhani³

et al. 2021

BMC Microbiol

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Background P. aeruginosa is the primary source of hospital-acquired infections. Unfortunately, antibiotic resistance is growing to precariously high levels, making the infections by this pathogen life-threatening and hard to cure. One possible alternative to antibiotics is to use phages. However, the isolation of phages suitable for phage therapy— be lytic, be efficient, and have a broad host range —against some target bacteria has proven difficult. To identify the best places to look for these phages against P. aeruginosa we screened hospital sewages, soils, and rivers in two cities. Results We isolated eighteen different phages, determined their host range, infection property, and plaque morphology. We found that the sewage and sewage-contaminated environments are the most reliable sources for the isolation of Pseudomonas phages. In addition, phages isolated from hospital sewage showed the highest efficiency in lysing the bacteria used for host range determination. In contrast, phages from the river had larger plaque size and lysed bacteria with higher levels of antibiotic resistance. Conclusions Our findings provided additional support for the importance of sewage as the source of phage isolation.

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Section: Introductionmentioning

confidence: 99%

Sewage and sewage-contaminated environments are the most prominent sources to isolate phages against Pseudomonas aeruginosa

Aghaee¹,

Mirzaei²,

Alikhani³

et al. 2021

BMC Microbiol

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“…Other than the nosocomial source, industrial wastewater effluents, hydrocarbon-contaminated sites, and composts are also suggested as overlooked hot spots for P. aeruginosa. , …”

Section: Resultsmentioning

confidence: 99%

Will a Non-antibiotic Metalloid Enhance the Spread of Antibiotic Resistance Genes: The Selenate Story

Shi

Liu

et al. 2020

Environ. Sci. Technol.

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The rapid emergence of antibiotic resistance genes (ARGs) has become an increasingly serious threat to public health. Previous studies illustrate the antibiotic-like effect of many substances. However, whether and how commonly used or existing non-antibiotic metalloids (e.g., selenate) would enhance ARG spread remains poorly known. Here, we tracked the long-term operation of a bioreactor continuously fed with selenate for more than 1000 days. Metagenomic sequencing identified 191 different ARGs, of which the total abundance increased significantly after the amendment of selenate. Network analyses showed that ARGs resisting multiple drugs had very similar co-occurrence patterns, implying a potentially larger health risk. Host classification not only indicated multidrug-resistant species but also distinguished the mechanism of ARG enrichment for vertical transfer and horizontal gene transfer. Genome reconstruction of an ARG host suggested that selenate and its bioreduction product selenite could stimulate the overproduction of intracellular reactive oxygen species, which was confirmed by the direct measurement. Bacterial membrane permeability, type IV pilus formation, and DNA repair and recombination were also enhanced, together facilitating the horizontal acquirement of ARGs. Overall, this study for the first time highlights the ARG emergence and dissemination induced by a non-antibiotic metalloid and identifies ARG as a factor to consider in selenate bioremediation.

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“…A study group in Hungary has published the occurrence of MDR P. aeruginosa found in environmental sites contaminated by hydrocarbons between the period of 2002-2007; carbapenem-resistance was noted in 33% of isolates (Kaszab et al 2010). The same study group has also recently reported ceftriaxone and imipenem resistance in 25.0% of tested environmental P. aeruginosa isolates; in addition, five out of the 44 isolates originating from sources as groundwater, soil or compost showed close genetic relatedness to clinically relevant pulse-field types based on pulse-field gel electrophoresis (PFGE) (Kaszab et al 2019). In a laboratory-based study, n = 250 carbapenem-resistant P. aeruginosa were surveyed for their susceptibilities against ceftazidime-avibactam (C/A) and ceftolozane-tazobactam (C/T), in addition to a phenotypic-genotypic study for carbapenemaseproduction: prevalence of resistance to C/A and C/T was 33.6% and 32.4%, respectively; isolates producing positive CIM-tests were VIM (80%) or NDM (11%) producers (O'Neall et al 2020).…”

Section: Gajdács Et Almentioning

confidence: 90%

Insights on carbapenem-resistant Pseudomonas aeruginosa

et al. 2021

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Pseudomonas aeruginosa (P. aeruginosa) is ubiquitous in nature, and may be a causative agent in severe, life-threatening infections. In >60% of cases, β-lactam antibiotics are used in the therapy of P. aeruginosa infections, therefore the emergence of carbapenem-resistant P. aeruginosa (CRPA) is a signiﬁcant clinical concern. In this study, phenotypic methods were used to characterize ﬁfty-four (n = 54) P. aeruginosa isolates, which were included based on their suspected non-susceptibility to meropenem. Minimum inhibitory concentrations (MICs) of meropenem, ceftazidime, cefepime, ciprofloxacin, gentamicin, were determined using E-tests, while colistin MICs were determined using broth microdilution. The isolates were subjected to the modiﬁed Hodge test (MHT), the modiﬁed carbapenem-inactivation method (mCIM) and the imipenem/EDTA combined disk test (CDT). AmpC and eﬄux pump overexpression was studied using agar plates containing cloxacillin and phenylalanine-arginine β-naphthylamide (PAβN), respectively. Assessment of bioﬁlm-formation was carried out using the crystal violet tube-adherence method. 38.9% of the strains showed meropenem MICs in the resistant range (>8 mg/L). Eﬄux-pump overexpression and AmpC-hyperproduction was seen in 44.4% and 35.2% of isolates, respectively. 88.8% of the isolates were characterized as strong bioﬁlm-producers. On the other hand, the presence of carbapenemases was suspected in a minority (16.7%) of tested isolates. As safe and eﬀective therapeutic options in carbapenem-resistant Gram-negative infections are severely limited, characterization of these isolates using phenotypic and molecular-based methods is important to provide insights into the epidemiological features of these pathogens.

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Groundwater, soil and compost, as possible sources of virulent and antibiotic-resistant Pseudomonas aeruginosa

Cited by 27 publications

References 64 publications

Sewage and sewage-contaminated environments are the most prominent sources to isolate phages against Pseudomonas aeruginosa

Sewage and sewage-contaminated environments are the most prominent sources to isolate phages against Pseudomonas aeruginosa

Will a Non-antibiotic Metalloid Enhance the Spread of Antibiotic Resistance Genes: The Selenate Story

Insights on carbapenem-resistant Pseudomonas aeruginosa

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