2009
DOI: 10.1128/jb.01755-08
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Group BStreptococcusPullulanase Crystal Structures in the Context of a Novel Strategy for Vaccine Development

Abstract: The group B streptococcus type I pullulanase (SAP) is a class 13 glycoside hydrolase that is anchored to the bacterial cell surface via a conserved C-terminal anchoring motif and involved in ␣-glucan degradation. Recent in vitro functional studies have shown that SAP is immunogenic in humans and that anti-SAP sera derived from immunized animals impair both group A and group B streptococcus pullulanase activities, suggesting that in vivo immunization with this antigen could prevent streptococcal colonization. T… Show more

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Cited by 31 publications
(25 citation statements)
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“…Several pullulanase-encoding genes have recently been cloned and characterized (12)(13)(14), and the crystal structures of a few pullulanases have been determined (19)(20)(21)(22). Pullulanase is usually composed of about 900 amino acids and has a molecular mass of about 100 kDa.…”
Section: ؊1mentioning
confidence: 99%
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“…Several pullulanase-encoding genes have recently been cloned and characterized (12)(13)(14), and the crystal structures of a few pullulanases have been determined (19)(20)(21)(22). Pullulanase is usually composed of about 900 amino acids and has a molecular mass of about 100 kDa.…”
Section: ؊1mentioning
confidence: 99%
“…Although the recovery of enzymes secreted into the periplasmic space can be achieved by various approaches (16,17), its application on an industrial scale has been limited by the unavailability of efficient large-scale methods for the selective release of periplasmic proteins from the cell (18). Therefore, a method that allows the secretion of recombinant pullulanase directly into the culture medium is highly desirable.Several pullulanase-encoding genes have recently been cloned and characterized (12)(13)(14), and the crystal structures of a few pullulanases have been determined (19)(20)(21)(22). Pullulanase is usually composed of about 900 amino acids and has a molecular mass of about 100 kDa.…”
mentioning
confidence: 99%
“…The KpPUL has an extra N-terminal carbohydrate binding module compared to its plant counterparts. Additionally two structures of Streptococci PULs from GH13_12 [21,22] and three structures of Bacilli PULs from GH13_14 are known [23][24][25]. None of the debranching enzyme structures published to date have been substrate complexes, i.e.…”
Section: Introductionmentioning
confidence: 99%
“…19 The structural properties of this potential vaccine candidate were characterized by analyzing the high-resolution crystal structures of a catalytically active, N-terminally truncated form of SAP (SAP-L), both in its apo form and in complex with maltotetraose (MTT). 16 The global three-dimensional structure of SAP-L (777 amino acids) shows an extended multi-domain fold; the apo and MTTbound forms are highly similar, since substrate binding does not induce significant conformational changes. Although such structures are a pre-requisite for mapping the protein surface, they fulfill only the early stages of the 'Bacabs pipeline,' and the precise epitopes of SAP that confer immunogenicity remain to be identified.…”
Section: 18mentioning
confidence: 99%
“…The first is a surface-exposed α-glucan degrading-enzyme from the class 13 glycoside hydrolase family, more specifically a type I pullulanase (SAP), from S. agalactiae (Group B Streptococcus, GBS). 16 Several lines of evidence now indicate that degradation of complex host-derived carbohydrates is crucial for bacterial virulence. In this context, GBS in Complement-Mediated Bactericidal Events: Towards a Global Approach to the Selection of New Vaccine Candidates", www.bacabs.org) was set up to address these questions.…”
mentioning
confidence: 99%