Group determination of 14-membered macrolide antibiotics and azithromycin using antibodies against common epitopes

Galvidis, Inna A.; Lapa, G. B.; Burkin, Maksim A.

doi:10.1016/j.ab.2014.09.009

Cited by 25 publications

(22 citation statements)

References 22 publications

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“…Recently, with the development of analytical instruments, various analytical methods have been gradually developed to determine the trace drug residues in complex matrices, including capillary electrophoresis (CE) [12–14], enzyme-linked immunosorbent assay (ELISA) [15], thin-layer chromatography (TLC) [16], voltammetric measurements [17], gas chromatography coupled to mass spectrometry (GC-MS) [18, 19], high-performance liquid chromatography (HPLC) [20–22], and liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) [2, 23–27]. Among these analytical methods, LC-MS/MS appears to be acknowledged as the most useful and authoritative methods for the quantification of MACs in complex matrices, due to its high sensitivity and good specificity [24, 26].…”

Section: Introductionmentioning

confidence: 99%

Determination of Six Macrolide Antibiotics in Chicken Sample by Liquid Chromatography-Tandem Mass Spectrometry Based on Solid Phase Extraction

Chen

Yin

Yang

et al. 2019

Journal of Analytical Methods in Chemistry

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In this paper, a simple and effective method for the determination of six macrolide antibiotics (MACs), including tylosin, tilmicosin, azithromycin, clarithromycin, roxithromycin, and kitasamycin, in the chicken sample using liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed based on a self-built porous aromatic framework- (PAF-) based solid phase sorbent. The main parameters influencing the extraction efficiency, such as sorbent amounts, type of the eluent, pH of the sample, and the eluent volume, were evaluated. Under the optimized condition, the limits of detection were from 0.2 to 0.5 μg·kg−1. The recoveries of the method ranged from 82.1% to 101.4% with the relative standard deviations less than 11.1%. All the results demonstrated that the established method is potential for the determination of macrolide antibiotics in food safety analysis and monitoring.

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Section: Introductionmentioning

confidence: 99%

Determination of Six Macrolide Antibiotics in Chicken Sample by Liquid Chromatography-Tandem Mass Spectrometry Based on Solid Phase Extraction

Chen

Yin

Yang

et al. 2019

Journal of Analytical Methods in Chemistry

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“…Possessing a broad specificity, such methods allow for the detection of any representative of the group of related compounds in the test sample. There are several known approaches that allow for the development of antibodies specific to common molecular determinants and the achievement of broad immunodetection:Targeting an immune response against common molecular determinants using a rationally designed immunizing hapten [8,11], keeping in mind the naturally produced antibodies;Screening combinatorial immune or synthetic libraries to target generic epitopes and improve the properties of recombinant antibodies, using maturation methods [12,13];Combining several antibodies with specificity that complement each other in a hybrid assay [14] or designing a bispecific antibody [15];Additional facilities for group recognition of analytes by polyclonal antibodies can be provided by the affinity fractionation approach, using the principle of hapten heterology. A structurally related analyte as a heterologous immobilized hapten can bind only to the portion of antiserum antibodies directed against common epitopes.…”

Section: Introductionmentioning

confidence: 99%

“…Targeting an immune response against common molecular determinants using a rationally designed immunizing hapten [8,11], keeping in mind the naturally produced antibodies;…”

Section: Introductionmentioning

confidence: 99%

Specific and Generic Immunorecognition of Glycopeptide Antibiotics Promoted by Unique and Multiple Orientations of Hapten

2019

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Conjugation chemistry does not always provide adequate spatial orientation of hapten in immunogens for the best presentation of generic or individual epitopes. In the present study, the influence of unique and multiple orientations of immunizing hapten on the immune response repertoire was compared to select generic recognition system. The glycopeptides, teicoplanin (TPL) and ristomycin (RSM), were conjugated to BSA to produce immunogens with unique and multiple orientations of haptens. Polyclonal antibodies generated against TPL conjugated through a single site were of uniform specificity and demonstrated selective TPL recognition, regardless of the coating conjugates design. The sensitivity (IC50) of 4 enzyme-linked immunosorbent assays (ELISAs) for TPL varied little within the 3.5–7.4 ng/mL, with a dynamic range of 0.2–100 ng/mL. RSM was coupled to BSA through several glycoside sites that evoked a wider repertoire of response. This first described anti-RSM antibody was selective for RSM in homologous hapten-coated ELISAs with IC50 values in the range 4.2–35 ng/mL. Among the heterologous antigens, periodate-oxidized TPL conjugated to gelatine was selected as the best binder of generic anti-RSM fraction. The developed ELISA showed group recognition of glycopeptides RSM, TPL, eremomycin, and vancomycin with cross-reactivity of 37–100% and a 10–10,000 ng/mL dynamic range. Thus, multiple presentations of immunizing hapten help expand the repertoire of immune responses and opportunities for the selection of the required fine-specificity agent.

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“…Besides, milk, egg and honey are also the interesting animal products for analysts. There are various methods that have been reported for the determination of macrolides in animal muscle samples, including enzyme-linked immunosorbent assay (ELISA) (Galvidis, Lapa, & Burkin, 2015), capillary electrophoresis (CE) (Zhou, Chen, & Cassidy, 2000), thin layer chromatography (TLC) (Ahmed, Sree, Abdel-Fattah, Hassan, & El-Dein Saad, 2013), high performance liquid chromatography (HPLC) (Lee, Yoo, & Shin, 2013), liquid chromatography-tandem mass spectrometry (LC-MS/MS) (Dickson, 2014). Among these analytical methods, HPLC and LC-MS/MS are the two most widely used and efficient methods due to the availability and high accuracy in different complex matrices.…”

Section: Introductionmentioning

confidence: 99%

Molecularly imprinted solid-phase extraction for the determination of ten macrolide drugs residues in animal muscles by liquid chromatography–tandem mass spectrometry

et al. 2016

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A simple and sensitive method based on molecularly imprinted solid-phase extraction coupled with liquid chromatography-tandem mass spectrometry was developed for the determination of the residues of ten macrolide drugs in swine, cattle and chicken muscles samples. The molecularly imprinted polymers (MIPs) were synthesized using tylosin as a template and methacrylic acid as a functional monomer. Samples were extracted with sodium borate buffer solution and ethyl acetate, and purified by the MIP cartridge. The results showed that the cartridge exhibited good recognition performance for macrolides, and better purification effect than the traditional solid-phase extraction cartridges. Recoveries of analytes at three spiking levels 1, 5 and 20μgkg(-1) ranged from 60.7% to 100.3% with the relative standard deviations less than 14%. The limits of detection of the method were between 0.1 and 0.4μgkg(-1). The method is useful for the routine monitoring of the residues of macrolide drugs in animal muscles.

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Group determination of 14-membered macrolide antibiotics and azithromycin using antibodies against common epitopes

Cited by 25 publications

References 22 publications

Determination of Six Macrolide Antibiotics in Chicken Sample by Liquid Chromatography-Tandem Mass Spectrometry Based on Solid Phase Extraction

Determination of Six Macrolide Antibiotics in Chicken Sample by Liquid Chromatography-Tandem Mass Spectrometry Based on Solid Phase Extraction

Specific and Generic Immunorecognition of Glycopeptide Antibiotics Promoted by Unique and Multiple Orientations of Hapten

Molecularly imprinted solid-phase extraction for the determination of ten macrolide drugs residues in animal muscles by liquid chromatography–tandem mass spectrometry

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