Instance segmentation is an accurate and reliable method to segment adhesive pigs’ images, and is critical for providing health and welfare information on individual pigs, such as body condition score, live weight, and activity behaviors in group-housed pig environments. In this paper, a PigMS R-CNN framework based on mask scoring R-CNN (MS R-CNN) is explored to segment adhesive pig areas in group-pig images, to separate the identification and location of group-housed pigs. The PigMS R-CNN consists of three processes. First, a residual network of 101-layers, combined with the feature pyramid network (FPN), is used as a feature extraction network to obtain feature maps for input images. Then, according to these feature maps, the region candidate network generates the regions of interest (RoIs). Finally, for each RoI, we can obtain the location, classification, and segmentation results of detected pigs through the regression and category, and mask three branches from the PigMS R-CNN head network. To avoid target pigs being missed and error detections in overlapping or stuck areas of group-housed pigs, the PigMS R-CNN framework uses soft non-maximum suppression (soft-NMS) by replacing the traditional NMS to conduct post-processing selected operation of pigs. The MS R-CNN framework with traditional NMS obtains results with an F1 of 0.9228. By setting the soft-NMS threshold to 0.7 on PigMS R-CNN, detection of the target pigs achieves an F1 of 0.9374. The work explores a new instance segmentation method for adhesive group-housed pig images, which provides valuable exploration for vision-based, real-time automatic pig monitoring and welfare evaluation.