Group I metabotropic glutamate receptor activation increases phosphorylation of cAMP response element-binding protein, Elk-1, and extracellular signal-regulated kinases in rat dorsal striatum

Choe, Eun Sang; Wang, John Q.

doi:10.1016/s0169-328x(01)00217-0

Cited by 70 publications

(65 citation statements)

References 45 publications

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“…In addition to dopamine (D 1 ) receptors, activation of group I mGluRs increases ERK1/2 phosphorylation in the spinal cord (Karim et al 2001), primary astrocytes (Schinkmann et al 2000), glial cell cultures (Peavy and Conn 1998), and the striatum (Choe and Wang 2001). Similarly, mGluR1a mediates phosphorylation of ERK2 in Chinese hamster ovary cells transfected with rat mGluRs (Ferraguti et al 1999) and rat cortical cultures (Fiore et al 1993).…”

Section: Discussionmentioning

confidence: 99%

“…In previous studies, the pCREB, pElk-1, pERK1/2, or Fos antiserum was adsorbed with pCREB, pElk-1, pERK1/2, or Fos peptide and immunostaining was blocked (Simpson et al 1995;Choe and McGinty 2000). As a control for pCREB-IR, CREB-IR was not induced by a cAMP analog or group I mGluR agonist (Choe and McGinty 2000;Choe and Wang 2001).…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…) and activates mitogen-activated protein kinases (MAPK) (Choe and Wang 2001). In contrast, activation of group II/III receptors downregulates the adenylate cyclase (AC) and cyclic adenosine monophosphate (cAMP) cascades.…”

mentioning

confidence: 99%

“…Stimulation of group I mGluRs with the selective agonist, 3,5-dihydroxyphenylglycine (DHPG), upregulates CREB, Elk-1, and ERK1/2 phosphorylation in the dorsal striatum (Choe and Wang 2001). Like DHPG, amphetamine increases CREB phosphorylation in the striatum (Konradi et al 1994).…”

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confidence: 99%

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Amphetamine Increases Phosphorylation of Extracellular Signal-regulated Kinase and Transcription Factors in the Rat Striatum via Group I Metabotropic Glutamate Receptors

Choe

Chung

Mao

et al. 2002

Neuropsychopharmacology

106

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Section: Discussionmentioning

confidence: 99%

Section: Immunohistochemistrymentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Amphetamine Increases Phosphorylation of Extracellular Signal-regulated Kinase and Transcription Factors in the Rat Striatum via Group I Metabotropic Glutamate Receptors

Choe

Chung

Mao

et al. 2002

Neuropsychopharmacology

106

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“…Stimulation of mGlu5 receptors results in activation of numerous signal transduction molecules including protein kinase C (PKC), mitogen-activated protein kinase (MAPK), Ca 2+ /calmodulin-dependent protein kinase (CaMK), extracellular signalregulated kinase (ERK), c-Jun N-terminal kinase (JNK), and the phosphoinositide-3-kinaseAkt-mammalian target of rapamycin (PI3K-Akt-mTOR) pathway (Hou and Klann, 2004;Liu et al, 2006;Peavy et al, 2001;Peavy and Conn, 1998;Yang et al, 2006). These enzymes can in turn activate DNA transcription factors such as cyclic adenosine monophosphate response element binding protein (CREB), nuclear factor κB (NFκB), c-Jun, and Elk-1 (Choe and Wang, 2001a;Liu et al, 2006;Wang, 2002, 2003a,b;Yang et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Transcriptional profiling of the rat frontal cortex following administration of the mGlu5 receptor antagonists MPEP and MTEP

Gass

2008

European Journal of Pharmacology

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The development of selective type 5 metabotropic glutamate receptor (mGlu5) antagonists, such as 2-methyl-6-(phenylethynyl)-pyridine (MPEP) and 3-[(2-methyl-1,3-thiazol-4-yl)ethynyl]-pyridine (MTEP), has revealed an important role for these receptors in various disorders of the nervous system including depression, anxiety, epilepsy, Parkinson's disease, drug addiction, and alcoholism. In this study, we used microarray technology to examine changes in gene expression induced by repeated administration of the mGlu5 antagonists MPEP and MTEP. Male Wistar rats (n=5 per treatment group) were administered MPEP (10 mg/kg), MTEP (10 mg/kg) or vehicle intraperitoneally twice daily for 5 days. Approximately 30 min following the final drug administration, rats were sacrificed and frontal cortices were then dissected and examined for changes in gene expression by cDNA microarray analysis. Changes in gene expression with P-values less than 0.01 were considered to be statistically significant. The expression of 63 genes was changed by both MPEP and MTEP, with 58 genes down-regulated and 5 genes up-regulated. Quantitative PCR verified the magnitude and direction of change in expression of 9 of these genes (r 2 =0.556, p=0.017). Pathway analysis revealed that many of the biological processes altered by repeated MPEP and MTEP treatment were related to ATP synthesis, hydrolase activity, and signaling pathways associated with mitogen-activated protein kinase (MAPK). Our results demonstrate diverse effects of MPEP and MTEP gene expression in the frontal cortex, and these results may help elucidate the mechanisms by which these compounds produce beneficial effects in animal models of various disorders of the central nervous system.

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Distinct expression of phosphorylated N‐methyl‐D‐aspartate receptor NR1 subunits by projection neurons and interneurons in the striatum of normal and amphetamine‐treated rats

Liu

Mao

Parelkar

et al. 2004

J of Comparative Neurology

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N-methyl-D-aspartate (NMDA) receptors are heteromeric assemblies of subunits (NR1 and NR2A-D), and are enriched in the striatum. Receptor phosphorylation has recently been demonstrated on the NR1 subunit at three serine residues, 897, 896, and 890, which appear to correspond to the level of receptor activity. In this study, expression of phospho-specific NR1 subunits at serine 897 (pNR1S897), serine 896 (pNR1S896), or serine 890 (pNR1S890) in neurochemically identified neurons of the adult rat striatum was detected by using double-immunofluorescent labeling or combined in situ hybridization and immunohistochemistry. In both the dorsal and ventral striatum, pNR1S897 was expressed at high levels in projection neurons containing >55% dynorphin (striatonigral) and >90% enkephalin (striatopallidal) and in interneurons that were 100% positive for choline, >90% positive for parvalbumin, and >45% positive for somatostatin (co-containing neuropeptide Y and neuronal nitric oxide synthase). Low levels of pNR1S896 were present in a small portion of projection neurons (<15% for both populations of projection neurons) and were almost lacking in the three types of interneurons. Interestingly, pNR1S890 was exclusively expressed in most parvalbumin-containing interneurons (70-80%). Acute administration of a psychostimulant, amphetamine, increased the number of dynorphin-containing projection neurons and parvalbumin interneurons showing detectable levels of pNR1S896 and pNR1S890, respectively. These results demonstrate the distinct expression of phospho-NR1 subunits in different populations of striatal projection neurons and interneurons at variable levels in normal rats; they also demonstrate that phosphorylation of NR1, at least on serine 896 and 890 sites, is sensitive to drug exposure.

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Group I metabotropic glutamate receptor activation increases phosphorylation of cAMP response element-binding protein, Elk-1, and extracellular signal-regulated kinases in rat dorsal striatum

Cited by 70 publications

References 45 publications

Amphetamine Increases Phosphorylation of Extracellular Signal-regulated Kinase and Transcription Factors in the Rat Striatum via Group I Metabotropic Glutamate Receptors

Amphetamine Increases Phosphorylation of Extracellular Signal-regulated Kinase and Transcription Factors in the Rat Striatum via Group I Metabotropic Glutamate Receptors

Transcriptional profiling of the rat frontal cortex following administration of the mGlu5 receptor antagonists MPEP and MTEP

Distinct expression of phosphorylated N‐methyl‐D‐aspartate receptor NR1 subunits by projection neurons and interneurons in the striatum of normal and amphetamine‐treated rats

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