Group-Targeting Detection of Total Steroid Estrogen Using Surface-Enhanced Raman Spectroscopy

Abstract

Steroid estrogens, including 17β-estradiol (TE2), estrone (TE1), and ethinyl estradiol (TEE2), which are the strongest endocrine disruptors and coexist in the environment, seriously harm the health of organisms; thus, the monitoring of total steroid estrogens (TEs) has attracted growing attention. Herein, a method based on surfaceenhanced Raman spectroscopy (SERS) group-targeting detection is established to detect TEs in natural water for the first time. The TEs response detection range and detection limit were 0.01−50 nM and 5 pM, respectively. An antiinterference ability was observed: even if coexisting interfering species were present in the system at 100-fold the concentration of estrogens, the detection error of the method was less than 0.276. In addition, the association constants between the aptamers and TE1, TE2, and TEE2 were similar, and therefore, the recognition of TE1, TE2, and TEE2 by the aptamers was consistent. Furthermore, the interaction sites A44, T72, and G69 between the aptamers and TE1, TE2, and TEE2 were investigated by molecular docking. On this basis, the estrogens in environmental water samples, including animal farm wastewater, maternity hospital wastewater, surface water from near an animal farm, and surface water from near a maternity hospital, were successfully determined. E strogens, a kind of environmental endocrine disruptor, are

Group-Targeting SERS Screening of Total Benzodiazepines Based on Large-Size (111) Faceted Silver Nanosheets Decorated with Zinc Oxide Nanoparticles

“…Among various drug screening methods, a newly developed technique, surface-enhanced Raman scattering (SERS), has attracted considerable attention due to its potential single-molecule sensitivity. , Also, this technique can directly provide structural information from the molecular fingerprint. The molecular specificity and amplification ability provide a powerful technological platform for the identification and quantification of trace analytes in a complex sample matrix without pretreatment. , It is well known that BZDs are structurally similar, possessing a seven-membered ring fused to an aromatic ring with four main substitution groups. Accordingly, it is feasible to conduct a group-targeting SERS screening method for quantitative detection of total BZDs using only one BZD as the reference standard, further reducing the cost and economizing resource.…”

“…The molecular specificity and amplification ability provide a powerful technological platform for the identification and quantification of trace analytes in a complex sample matrix without pretreatment. 9,10 It is well known that BZDs are structurally similar, possessing a seven-membered ring fused to an aromatic ring with four main substitution groups. Accordingly, it is feasible to conduct a group-targeting SERS screening method for quantitative detection of total BZDs using only one BZD as the reference standard, further reducing the cost and economizing resource.…”

Detection of Formaldehyde by Surface-Enhanced Raman Spectroscopy Based on PbBiO2Br/Au4Ag4 Nanospheres

“…Surface-enhanced Raman spectroscopy (SERS) has attracted increased attention owing to its great features of sensitivity, rapidity, and nondestructive nature. − Therefore, SERS is an ideal spectral analysis method that can meet the high demands of on-site, rapid, sensitive, and selective screening of contaminants in the environment. − The introduction of SERS substrates with enhancement effects is crucial for SERS analysis. Thus far, many environmental contaminants have been analyzed via SERS based on different substrates.…”

Visual Test Paper for on-Site Polychlorinated Biphenyls Detection and Its Logic Gate Applications

“…Since aptamers have the advantages of good stability, easy synthesis, high affinity, and excellent specificity, they are widely used in the construction of biosensors for the detection of a series of pollutants. 12,13 Recently, several groups have developed some elegant sensing systems for PCB77 detection using aptamers as molecular recognition components to design electrochemical biosensors, 14−17 fluorescent detectors, 18,19 colorimetric assays, 20 and Raman scattering assays. 21−23 Although those detection platforms are effective, most of them require laboratory-based instruments, which may limit their applications for portable and on-site screening of PCB77 in resource-constrained settings.…”

“…Since aptamers have the advantages of good stability, easy synthesis, high affinity, and excellent specificity, they are widely used in the construction of biosensors for the detection of a series of pollutants. , Recently, several groups have developed some elegant sensing systems for PCB77 detection using aptamers as molecular recognition components to design electrochemical biosensors, − fluorescent detectors, , colorimetric assays, and Raman scattering assays. − Although those detection platforms are effective, most of them require laboratory-based instruments, which may limit their applications for portable and on-site screening of PCB77 in resource-constrained settings. As an ideal alternative, the lateral flow test paper has attracted increasing attention due to its portability, cost-effectiveness, fast response, and ease of use. , A typical lateral flow paper consists of sample pad, conjugate pad, nitrocellulose membrane, and absorption pad. , The results in the test paper sensing platform can be easily observed by the naked eye using gold nanoparticles as tracers .…”