Grouping and Cross-Reacting Antigens of Oral Lactic Acid Bacteria

Kw, Knox; Aj, Wicken

doi:10.1177/002203457605500104011

Cited by 13 publications

(3 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast, it is the lipoteichoic acid which is the major immunogen for L. fermentum and the cell wall polysaccharide component is a very weak immunogen (9). The reason for these distinctions is not apparent, particularly as the wall 12 polysaccharides of L. casei strains are strongly immunogenic (13). The presence of associated cell surface proteins may be important in influencing immunogenicity, because trypsinizing L. plantarum cell wall results in the loss of immunogenicity of the ribitol teichoic acid component but not of the contaminating lipoteichoic acid in such preparations (12).…”

mentioning

confidence: 99%

Serological studies on chemostat-grown cultures of Lactobacillus fermentum and Lactobacillus plantarum

Knox¹,

Campbell²,

Broady³

et al. 1979

Infect Immun

View full text Add to dashboard Cite

Lactobacillus fermentum NCTC 6991 and Lactobacillus plantarum NCIB 7220 were grown in a chemostat in the diffusible fraction of complex medium at pH 6.0 with glucose limitation. Organisms grown at different dilution rates (D) were injected into rabbits, and the resultant antisera were examined for reactivity with antigens previously isolated from batch-grown organisms. For L. fermentum, antisera obtained on injecting cells grown at D = 0.5 h-' contained a significantly higher level of antibody reacting with lipoteichoic acid and a lower level of antibody reacting with wall polysaccharide than did antisera obtained with slower-growing cells (D = 0.05 and 0.033). Antibodies to the cell wall polysaccharide were a-D-glucosyl specific and cross-reacted with dextran and a-D-glucosyl ribitol teichoic acid from L. plantarum. The immunogenicity of the ribitol teichoic acid and lipoteichoic acid components of L. plantarum was not influenced by injecting organisms grown at different rates. However, chemical and serological studies indicate that growth of L. plantarum in the diffusible fraction of complex medium results in a wall teichoic acid of lower glucose substitution. This apparently influences the specificity of the resultant antibodies so that some sera react much less with glucosyl-substituted lipoteichoic acid and dextran.Most strains of lactobacilli can be identified on July 15, 2020 by guest http://iai.asm.org/ Downloaded from

show abstract

mentioning

confidence: 99%

Serological studies on chemostat-grown cultures of Lactobacillus fermentum and Lactobacillus plantarum

Knox¹,

Campbell²,

Broady³

et al. 1979

Infect Immun

View full text Add to dashboard Cite

show abstract

“…Studies on the identification of plaque lactobacilli based on their grouping antigens were initiated when I rejoined the Institute in 1958 and, with Tony Wicken (University of NSW), led to the discovery of lipoteichoic acid (LTA) (Wicken and Knox, 1975;Knox and Wicken, 1976). It may function as a grouping antigen, as for group F lactobacilli, or as a "common antigen" when the glycerol phosphate chain is unsubstituted with carbohydrate, and also displays a range of biological properties, many being shared with LPS (Wicken and Knox, 1980).…”

Section: Microbial Ecology and Pathogenicitymentioning

confidence: 99%

Neil Goldsworthy and His Legacy: Sydney's Institute of Dental Research

1999

J Dent Res

View full text Add to dashboard Cite

Dr. Neil Goldsworthy had a profound effect on the development of dental research in the biological sciences in Australia, including measures for the prevention of dental caries. He played a major role in the establishment of the Institute of Dental Research in Sydney in 1946 and was Director until his sudden death in 1960. Reviewing the Institute's activities provides the opportunity for his achievements to receive due recognition and to show how they influenced its subsequent development. As one who has been associated with the Institute for most of the last 50 years, it is an honor for me to undertake this task.

show abstract

“…The application of peptidoglycan (murein) type as a taxonomic criterion for Gram-positive bacteria was investigated by Cummins and Harris (1956) and reviewed by . Antibodies prepared against cell wall polysaccharides, cell wall teichoic acids and membrane teichoic acids are used to differentiate bacteria into various antigenic groups (Sharpe, 1970;Knox and Wicken, 1976). Thus far no antibodies were raised against the cell wall components in Leuconostoc spp.…”

Section: Cell Wall Compositionmentioning

confidence: 99%