Grouping of enterotoxigenic Escherichia coli by hydrophobicity and its relation to hemagglutination and enterotoxin productions

Honda, Toru

doi:10.1016/0378-1097(83)90272-0

Cited by 16 publications

(24 citation statements)

References 1 publication

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“…Two different assays demonstrated that the wild‐type strains are more hydrophobic than the Nod – mutant strains. For example, in the salt aggregation test (Honda et al ., 1983), both Rm1021 (Fig. 6B) and RCR2011 (Fig.…”

Section: Resultsmentioning

confidence: 99%

Rhizobium common nod genes are required for biofilm formation

Fujishige

Lum

Hoff³

et al. 2007

Molecular Microbiology

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SummaryIn legume nitrogen-fixing symbioses, rhizobial nod genes are obligatory for initiating infection thread formation and root nodule development. Here we show that the common nod genes, nodD1ABC, whose products synthesize core Nod factor, a chitin-like oligomer, are also required for the establishment of the three-dimensional architecture of the biofilm of Sinorhizobium meliloti. Common nod gene mutants form a biofilm that is a monolayer. Moreover, adding Nod Factor antibody to S. meliloti cells inhibits biofilm formation, while chitinase treatment disrupts pre-formed biofilms. These results attest to the involvement of core Nod factor in rhizobial biofilm establishment. However, luteolin, the plant-derived inducer of S. meliloti's nod genes, is not required for mature biofilm formation, although biofilm establishment is enhanced in the presence of this flavonoid inducer. Because biofilm formation is plant-inducerindependent and because all nodulating rhizobia, both alpha-and beta-proteobacteria have common nod genes, the role of core Nod factor in biofilm formation is likely to be an ancestral and evolutionarily conserved function of these genes.

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Section: Resultsmentioning

confidence: 99%

Rhizobium common nod genes are required for biofilm formation

Fujishige

Lum

Hoff³

et al. 2007

Molecular Microbiology

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“…It has also been shown that strains of E. coli with colonization factor antigens have high surface hydrophobicity [29]. We previously examined 626 strains of Aeromonas isolated from various sources for hydrophobicity.…”

Section: Discussionmentioning

confidence: 99%

Mannose-resistant adhesion of motileAeromonasto INT407 cells and the differences among isolates from humans, food and water

1991

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SUMMARYAeromonas has been recognized as an important enteropathogen, but factors related to its virulence have not been clarified. For most enteric pathogens, attachment is a prerequisite for infection and for the effective delivery of toxins to the intestinal epithelial cells. We examined a total of 273 strains isolated from stool, food and environmental specimens by an assay for mannose-resistant adhesion to INT407 cells in vitro. Seventeen of the 102 faecal isolates were adhesive strains with more than 10 bacteria adhering per cell, while only 2 of the 118 isolates from foods and river water adhered to the cells (P < 0001). It is possible that the adhesion might serve as a marker for discrimination between the pathogenic and nonpathogenic isolates. The 8 highly adhesive strains with more than 20 adhering organisms per cell were scrutinized for the mechanism of adhesion. No correlation was apparent between the adhesion to INT407 cells and hydrophobicity. It was noted that fucose inhibited the adhesion of four strains as well as haemagglutination by them. Electronmicroscopic studies showed the presence of flexible and curvilinear fimbriae in only 2 of the 8 highly adhesive strains.

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“…Adherence of £". coli to intestinal mucosa mediated by colonisation factors with recognised patterns of haemagglutination (Evans, Evans and DuPont, 1979b) or other adhesins (Thomas et aL, 1982;Honda et al, 1983) precedes the development of diarrhoea caused by enterotoxigenic E. coli (Satterwhite et aL, 1978). For Aeromonas spp., mechanisms of pathogenesis are not defined, although analogy with known enteric pathogens suggests these bacteria may produce several possible virulence factors (Burke and Gracey, 1986).…”

Section: Discussionmentioning

confidence: 99%

Haemagglutination Patterns of Aeromonas Spp. Related to Species and Source of Strains

Burke¹,

Cooper²,

Robinson³

1986

Aust J Exp Biol Med

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The study included 138 A. sobria and 182^1. hydrophila isolated in Perth from samples of diarrhoeal or non-diarrhoeal faeces or from domestic water. Strains were grouped in relation to agglutination of human, horse, rat and guinea pig erythrocytes and the effect of sugars on haemagglutination. Agglutination of red cells of all four species (primary group 1) was most commonly associated with A. sobria, particularly those strains isolated from faeces of patients with diarrhoea. Most A. hydrophila associated with diarrhoea also belonged to group 1 but A. hydrophila from non-diarrhoeal stools or from water most commonly agglutinated human and guinea pig cells but not horse erythrocytes (primary groups 2 and 3). Fucose-resistant haemagglutination (FRHA) of strains in primary group 1 occurred with about 68% of the strains of A. sobria associated with diarrhoea. Mannose-resistant haemagglutination (MRHA) was limited to 29"/' o of strains of A. sobria associated with diarrhoea. The predominance of primary group 1 among strains of Aeromonas spp. associated with diarrhoea and the proportion of these strains showing FRHA suggest that haemagglutination of cells from human, horse, rat and guinea pig, particularly if fucoseresistant, should be considered in a search for characteristics, which possibly contribute to virulence of Aeromonas spp.Abbreviations used in this paper: FRHA, Fucose-resistant haemagglutination; FSHA, fucose-sensitive haemagglutination; MRHA, mannose-resistant haemagglutination; MSHA, Mannose-sensitive haemagglutination; PBS, Phosphate-buffered saline.

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Grouping of enterotoxigenic Escherichia coli by hydrophobicity and its relation to hemagglutination and enterotoxin productions

Cited by 16 publications

References 1 publication

Rhizobium common nod genes are required for biofilm formation

Rhizobium common nod genes are required for biofilm formation

Mannose-resistant adhesion of motileAeromonasto INT407 cells and the differences among isolates from humans, food and water

Haemagglutination Patterns of Aeromonas Spp. Related to Species and Source of Strains

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