Groups of a-D- Galactosyltransferase Activity in Sera of
Individuals with Normal B Phenotype

Badet, J.; Ropars, C.; Cartron, J.P.; Doinel, C.; Salmon, C.

doi:10.1159/000465540

Cited by 4 publications

(8 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Besides, enzyme activities of Aj and A2 subjects overlap widely. The large range of enzyme activities observed among Aj individuals do not seem to be related to the existence of distinct groups of transferase activities, similar to those described by Badet et al [1] for the a-D-galactosyltransferases detected in the sera of subjects of blood group B.…”

Section: Discussionsupporting

confidence: 87%

See 1 more Smart Citation

Assay of α-TV-Acetylgalactosaminyltransferases in Human Sera

Cartron¹,

Gerbal²,

Badet³

et al. 1975

Vox Sanguinis

Self Cite

View full text Add to dashboard Cite

The study of the α-N-acetylgalactosaminyltransferase in the sera of 19 individuals belonging to the rare A(m) blood group makes it possible to confirm the heterogeneity of this phenotype established on genetical and immunological criteria. Two groups of subjects, A(m) and A(y), can be distinguished. For the individuals of the first group, named A(m), 15 samples (7 families) have been studied, the phenotype is inherited as an allele at the ABO locus. 14 of these subjects, have an α-N-acetylgalactosaminyltransferase whose kinetic properties were similar to those of A(1) subjects. In one family, however, the A transferase detected is of the A(2) type. On a quantitative level, the enzyme activities of these sera only reached 30-50% of the average value observed for A(1) or A(2) subjects, respectively. These facts suggest the existence of a genetic inhibitor, possibly linked to the ABO locus, preventing either an A(1) or A(2) gene from acting at the level of some cellular lines and leading therefore to the recognition of phenotypes named A(m)^A1 and A(m)^A2. On the contrary, under the experimental conditions used, no α-N-acetylgalactosaminyltransferase activity was detected among the four individuals of the second group, named Ay by Weiner et al. [37], and whose appeareance in siblings results from the action of a recessive modifying y^A gene.

show abstract

Section: Discussionsupporting

confidence: 87%

“…On the other hand, the a-Dgalactosyl transferase activity of AmB individuals sera tested according to a technique described elsewhere [1] was found to be normal. However, one Am sample (HOE.…”

Section: Measurements Of Glycosyltransferase Activities On 2'fl Studymentioning

confidence: 89%

Assay of α-TV-Acetylgalactosaminyltransferases in Human Sera

Cartron¹,

Gerbal²,

Badet³

et al. 1975

Vox Sanguinis

Self Cite

View full text Add to dashboard Cite

show abstract

“…18 hr incubation process with 2'FL. The amount of A or B [14C]tetra~accharide* synthesized after 18 hr of incubation at 37°C by 10 p1 of A or B human sera in the presence of 2'FL in standard conditions of assay has already been described (Badet et al, 1974;Cartron et al, 1975). 72 hr incubation process with 2'FL or human H substance.…”

Section: A and B Blood Group Glycosyltransferase Assays In Seramentioning

confidence: 99%

“…Ident8cation of reaction products. Following the incubation process of 18 to 72 hr, the A and/or B tetrasaccharides synthesized from 2'FL were isolated and identified as previously described (Badet et al, 1974;Cartron et al, 1975). The N-acetyl-~-[-l-~~C]galactosamine incorporated onto the H substance was estimated after high voltage electrophoresis in 0.1 M ammonium formate buffer, pH 3-5 (Whatman paper No.…”

Section: A and B Blood Group Glycosyltransferase Assays In Seramentioning

confidence: 99%

STUDY OF THE Α‐N‐ACETYLGALACTOSAMINYLTRANSFERASE IN SERA AND RED CELL MEMBRANES OF HUMAN a SUBGROUPS

Cartron

Badet

Mulet

et al. 1978

Int J Immunogenetics

Self Cite

View full text Add to dashboard Cite

SUMMARY The product of the A blood group gene in the erythrocyte membrane and serum from ‘weak A’ variants was investigated using low and high molecular weight acceptors and compared with common A1 and A2 blood samples. A reduced enzyme activity was detected, but only in some of the A variants, namely the A3 (eight out of eleven), Am or Ay samples. Other sera from A3 (three out of eleven), Ax, Aend or Ael individuals were apparently devoid of enzyme activity. A kinetic study by temperature inactivation of A blood group sera also showed that A enzymes are more labile than B enzymes from BI or BII sera. Moreover, in one case (A3 sample Del.), a very fast inactivation of the A enzyme was observed, suggesting the occurrence of a variant enzyme qualitatively different from the others so far studied. The erythrocyte membrane preparations from all A variants contained no detectable A enzyme activity except for the A1 and A2 samples, the former being three to five times more active than the latter. The B enzyme activities from four samples of B RBC tested were comparatively stronger than the A enzyme activity of A1 RBC. The results were discussed and it was suggested that the synthesis and/or the secretion of the A enzyme in the organism is not uniform from one tissue to another, but could depend on which of the ‘weak A’ alleles or modifier genes is involved.

show abstract

“…Badet et al [1] report great variation in transferase activity, and ascribe observed discrepancies between transferase activity and red cell agglutinability to the indepen dent action of B and H genes, resulting in variation of the red cell antigens. They further suggest that combinations of dif ferent transferase activities may give rise to observed differences between racial groups.…”

Section: Resultsmentioning

confidence: 99%

H, A and Ai Reactivity in Two Polynesian Groups

Booth¹,

Cartwright²

1977

Vox Sanguinis

View full text Add to dashboard Cite

Polynesian O bloods from Samoans and Tokelau Islanders have the same mean H reactivity as Caucasian, and their A(1) bloods the same A(1) content. However, Polynesian A(1) bloods have significantly greater H reactivity than Caucasian, and the Tokelau Islanders also showed enhanced A and I^T. It is suggested that the Polynesian AHI molecular conformation must differ from the Caucasian pattern, providing an arrangement of antigen sites more favourable to some antigen-antibody reactions.

show abstract

Groups of a-D- Galactosyltransferase Activity in Sera of Individuals with Normal B Phenotype

Cited by 4 publications

References 10 publications

Assay of α-TV-Acetylgalactosaminyltransferases in Human Sera

Assay of α-TV-Acetylgalactosaminyltransferases in Human Sera

STUDY OF THE Α‐N‐ACETYLGALACTOSAMINYLTRANSFERASE IN SERA AND RED CELL MEMBRANES OF HUMAN a SUBGROUPS

H, A and Ai Reactivity in Two Polynesian Groups

Contact Info

Product

Resources

About